Transcriptional Profiling of Sorghum Induced by Methyl Jasmonate, Salicylic Acid, and Aminocyclopropane Carboxylic Acid Reveals Cooperative Regulation and Novel Gene Responses  

Abstract

We have conducted a large-scale study of gene expression in the C4 monocot sorghum (Sorghum bicolor) L. Moench cv BTx623 in response to the signaling compounds salicylic acid (SA), methyl jasmonate (MeJA), and the ethylene precursor aminocyclopropane carboxylic acid. Expression profiles were generated from seedling root and shoot tissue at 3 and 27 h, using a microarray containing 12,982 nonredundant elements. Data from 102 slides and quantitative reverse transcription-PCR data on mRNA abundance from 171 genes were collected and analyzed and are here made publicly available. Numerous gene clusters were identified in which expression was correlated with particular signaling compound and tissue combinations. Many genes previously implicated in defense responded to the treatments, including numerous pathogenesis-related genes and most members of the phenylpropanoid pathway, and several other genes that may represent novel activities or pathways. Genes of the octadecanoic acid pathway of jasmonic acid (JA) synthesis were induced by SA as well as by MeJA. The resulting hypothesis that increased SA could lead to increased endogenous JA production was confirmed by measurement of JA content. Comparison of responses to SA, MeJA, and combined SA+MeJA revealed patterns of one-way and mutual antagonisms, as well as synergistic effects on regulation of some genes. These experiments thus help further define the transcriptional results of cross talk between the SA and JA pathways and suggest that a subset of genes coregulated by SA and JA may comprise a uniquely evolved sector of plant signaling responsive cascades.

Induction of a leaf specific geranylgeranyl pyrophosphate synthase and emission of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene in tomato are dependent on both jasmonic acid and salicylic acid signaling pathways

“…We therefore conclude that JA and SA can both induce LeGGPS1 and that basic levels of SA are essential for the induction of LeGGPS1. Other examples of genes that are induced by JA-and SA-treatment have been described for sorghum (Salzman et al 2005). Induction of several genes from Arabidopsis is also dependent on both JA-and SA-signaling pathways (Glazebrook et al 2003).…”

Salicylic Acid Suppresses Jasmonic Acid Signaling Downstream of SCFCOI1-JAZ by Targeting GCC Promoter Motifs via Transcription Factor ORA59

“…The effect of SA and MeJA on gene expression has been analyzed in several small-and large-scale microarray studies in Arabidopsis and Sorghum bicolor (Schenk et al, 2000;Salzman et al, 2005). In addition, global expression phenotyping of signaling-defective mutants of the SA and JA pathways has been exploited to investigate the network of regulatory interactions among different defense signaling pathways (Glazebrook et al, 2003).…”

Patterns of expression of lipid-transfer protein gene in organs of Scots pine (Pinus sylvestris L.)

“…No. ВМ 318308), описано у Sorghum bicolor [22], але негативна регуляція цими стресовими гормонами експресії гена LTP у рослин нами виявлена вперше. Вва-жається, що регуляція експресії мультигенної родини генів LTP у голонасінних від-різняється від такої у покритонасінних [15].…”