Transcriptional Regulation Involved in Fear Memory Reconsolidation

Wang, Xu; Li, Min; Zhu, Haitao; Yu, Yongju; Xu, Yuanyuan; Zhang, Wenmo; Bian, Chen

doi:10.1007/s12031-018-1084-4

Cited by 8 publications

(6 citation statements)

References 153 publications

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“…The BLA is a key limbic structure for the acquisition, persistence, and loss of fear memory 28 , 29 . To determine if losartan directly affects immediate early genes (IEGs) expression, we first performed RT-qPCR on BLA tissue, for Fos, Arc and Egr-1, which are essential for the consolidation of fear memories 30 . Using both RT-qPCR and RNA-sequencing, we examined the effects of peripheral losartan on gene transcript changes in the BLA during reconsolidation.…”

Section: Resultsmentioning

confidence: 99%

“…Using both RT-qPCR and RNA-sequencing, we examined the effects of peripheral losartan on gene transcript changes in the BLA during reconsolidation. We first performed RT-qPCR on BLA tissue to identify activation of immediate early-genes (IEGs): Fos , Arc , and Egr-1 , which are essential for the consolidation of fear memories 30 . Irrespective of drug treatment, both retrieval groups showed increased expression (2–3 fold-change) compared to the non-retrieval (NR or “control”) group (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Evaluation of an angiotensin Type 1 receptor blocker on the reconsolidation of fear memory

Swiercz

Iyer

et al. 2020

Transl Psychiatry

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Inhibition of the angiotensin type 1 receptor (AT1R) has been shown to decrease fear responses in both humans and rodents. These effects are attributed to modulation of extinction learning, however the contribution of AT1R to alternative memory processes remains unclear. Using classic Pavlovian conditioning combined with radiotelemetry and whole-genome RNA sequencing, we evaluated the effects of the AT1R antagonist losartan on fear memory reconsolidation. Following the retrieval of conditioned auditory fear memory, animals were given a single intraperitoneal injection of losartan or saline. In response to the conditioned stimulus (CS), losartan-treated animals exhibited significantly less freezing at 24 h and 1 week; an effect that was dependent upon memory reactivation and independent of conditioned cardiovascular reactivity. Using an unbiased whole-genome RNA sequencing approach, transcriptomic analysis of the basolateral amygdala (BLA) identified losartan-dependent differences in gene expression during the reconsolidation phase. These findings demonstrate that post-retrieval losartan modifies behavioral and transcriptomic markers of conditioned fear memory, supporting an important regulatory role for this receptor in reconsolidation and as a potential pharmacotherapeutic target for maladaptive fear disorders such as PTSD.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Evaluation of an angiotensin Type 1 receptor blocker on the reconsolidation of fear memory

Swiercz

Iyer

et al. 2020

Transl Psychiatry

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“…One important limitation of our work is that we did not use memory interference as confirmation for memory labilization. Instead we chose to assess the change in levels of plasticity markers associated with memory labilization, such as Zif268 and GluN2B (Tronson & Taylor, 2007; Wang et al, 2009, 2018), as this way we could extract more information using the same animals, including correlating oscillatory patterns with molecular plasticity. We also took into account the results of a recent systematic review of the literature which pointed that post interference amnesia is still very little understood and a failure in producing this effect may be related to laboratory procedures and other related factors (Schroyens et al, 2021) and may not always be a perfect indicator for memory labilization.…”

Section: Discussionmentioning

confidence: 99%

Aversive memory reactivation: A possible role for delta oscillations in the hippocampus–amygdala circuit

Couto-Pereira

Zanona

Bernardi

et al. 2022

J of Neuroscience Research

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Memory labilization, the process by which memories become susceptible to update, is essential for memory reconsolidation and has been a target for novel therapies for traumatic memory-associated disorders. Maternal separation (MS) in male rats produced memories resistant to labilization in adulthood. Based on previous results, we hypothesized that temporal desynchronization between the dorsal hippocampus (DHc) and the basolateral amygdala (BLA), during memory retrieval, could be responsible for this impairment. Our goal was to investigate possible differences in oscillatory activity and synchrony between the DHc and BLA during fear memory reactivation, between MS and non-handled (NH) rats. We used male adult Wistar rats, NH or MS, with electrodes for local field potential (LFP) recordings implanted in the DHc and BLA. Animals were submitted to aversive memory reactivation by exposure to the conditioned context (Reat) or to pseudo-reactivation in a neutral context (pReat), and LFP was recorded. Plasticity markers linked to reconsolidation were evaluated one hour after reactivation. The power of delta oscillations and DHc-BLA synchrony in Reat animals was increased, during freezing. Besides, delta modulation of gamma oscillations amplitude in the BLA was associated with the increase in DHc Zif268 levels, an immediate early gene specifically associated with reconsolidation.Concerning early life stress, we found lower power of delta and strength of deltagamma oscillations coupling in MS rats, compared to NH, which could explain the low Zif268 levels in a subgroup of MS animals. These results suggest a role for delta oscillations in memory reactivation that should be further investigated.

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“…Memory reconsolidation involves complex transcriptional and translational processes crucial for stabilizing memories [63][64][65] . For example, our recent RNA sequencing analysis in mice revealed transcriptional changes in the dorsal hippocampus and mPFC during contextual alcohol memory reconsolidation 15 .…”

Section: A Unique Transcriptional Fingerprint Within Alcohol Memory N...mentioning

confidence: 99%

Transcriptional analysis of neuronal ensembles of alcohol memories within the nucleus accumbens

Aronovici,

Prilutski,

Urshansky

et al. 2023

Preprint

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Alcohol-associated memories play an important role in relapse in alcohol use disorder. Disrupting these memories, which become labile upon retrieval, through interference with their reconsolidation process, could reduce relapse. Memories are thought to be encoded within specific patterns of sparsely distributed neurons, called neuronal ensembles. Here, we explored the role of neuronal ensembles in alcohol-memory reconsolidation and relapse and characterized their transcriptional signature. Upon retrieving alcohol-related memories, we observed increased neuronal activation in the nucleus accumbens (NAc). We established the causal role of these NAc ensembles in alcohol-memory reconsolidation using the Daun02 method with the Fos-LacZ transgenic rat, which expresses β-galactosidase (β-gal) under the Fos promoter, allowing the selective ablation of activated neurons. Selective inactivation of the active NAc neuronal ensemble produced a long-lasting attenuation of relapse. Through fluorescence-activated cell sorting (FACS) and RNA sequencing, we found a unique transcriptional fingerprint in activated Fos-positive neuronal ensembles in NAc following alcohol memory retrieval (vs. no retrieval controls) that was not present in the Fos-negative neurons. Our findings underscore the critical role of NAc neuronal ensembles in alcohol-associated memory reconsolidation. These neurons have a unique transcriptional profile that can provide novel targets for reducing alcohol relapse.

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Transcriptional Regulation Involved in Fear Memory Reconsolidation

Cited by 8 publications

References 153 publications

Evaluation of an angiotensin Type 1 receptor blocker on the reconsolidation of fear memory

Evaluation of an angiotensin Type 1 receptor blocker on the reconsolidation of fear memory

Aversive memory reactivation: A possible role for delta oscillations in the hippocampus–amygdala circuit

Transcriptional analysis of neuronal ensembles of alcohol memories within the nucleus accumbens

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