Transcriptional regulation of two RTE‐like genes of carnation during flower senescence and upon ethylene exposure, wounding treatment and sucrose supply

Abstract

RTE1 (REVERSION-TO-ETHYLENE SENSITIVITY1) was identified as a positive regulator of ETR1 (ethylene resistant1) function in Arabidopsis; RTEs are a small gene family. Ethylene plays a crucial role in the senescence of carnation (Dianthus caryophyllus L.) flowers. Two cDNA clones encoding putative RTE-like protein (DCRTE1 and DCRTH1) were obtained from total RNA isolated from senescing carnation petals using RT-PCR and RACE techniques. The predicted proteins of DCRTE1 and DCRTH1 consist of 228 and 233 amino acids, respectively. Interestingly, the deduced DCRTE1 protein, like most other RTEs, includes two putative transmembrane domains, while the deduced DCRTH1 protein includes five putative transmembrane domains, according to the TMHMM database. Northern blots showed that the level of DCRTE1 mRNA in petals first decreased then increased remarkably after ethylene production started, and DCRTE1 expression showed an increasing trend in ovaries during natural flower senescence. The amount of DCRTH1 transcripts increased gradually in both petals and ovaries during natural senescence. Exogenous ethylene increased transcript abundance of DCRTE1 and DCRTH1 to various degrees in both petals and ovaries. STS treatment decreased the level of DCRTH1 mRNA in petals and ovaries compared with the control. DCRTE1 and DCRTH1 showed a rapid increase and then a decrease in mRNA accumulation in leaves after wounding. These results suggest that both DCRTE1 and DCRTH1 could play important roles in flower senescence-related signalling. Sucrose treatment did not remarkably affect the amount of DCRTE1 and DCRTH1 mRNAs.

Arabidopsis CPR5 regulates ethylene signaling via molecular association with the ETR1 receptor

“…In Rosa hybrida , the expression of Rh‐RTH1 was responsive to ethylene, and was also partially correlated with expression of Rh‐ETR1 and Rh‐ETR3 (Yu et al ). In addition, it was reported that the rice RTE1 homolog ( OsRTH1 , Zhang et al ) and the RTE ‐like genes ( DCRTE1 and DCRTH1 ) of carnation (Yu et al ) participate in the regulation of ethylene responses in seedling growth and flower senescence.…”

The chromosome-level genome of Gypsophila paniculata reveals the molecular mechanism of floral development and ethylene insensitivity

“…For instance, AGa and AGb , the AG subfamily, are widely expressed in stamens and carpels in most flower plants, such as Nymphaea colorata , D. caryphyllus , and Eustoma grandiflorum , and the mutation or reduced expression in petal of AG will cause the formation of double flower [ 39 – 41 ]. Moreover, it has been reported that mutations in the miR172 target site of AP2 / PET were responsible for the double flower formation in Arabidopsis , petunia, rose, peach, and Dianthus [ 42 ]. In present study, we detected an SNP substitution in PETa from two double flower cultivars, but no mutations in EMS mutants, which had fewer petals compared to the cultivar.…”

PhCESA3 silencing inhibits elongation and stimulates radial expansion in petunia

“… 33 . The partial sequences of PhCESA3 were obtained using a computational identification approach 34 . Briefly, TBLASTN analysis against the GenBank EST database ( http://www.ncbi.nlm.nih.gov ) with A. thaliana CESA3 identified 1 petunia clone, FN016492, which encodes putative proteins that displayed conservation with A. thaliana CESA3.…”