Transcriptional regulatory proteins as biosensing tools

Turner, Kendrick B.; Joel, Smita; Feliciano, Jessika; Feltus, Agatha; Pasini, Patrizia; Wynn, Daniel T.; Dau, Peter C.; Dikici, Emre; Deo, Sapna K.; Daunert, Sylvia

doi:10.1039/c6cc09512g

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…Therefore, it is an important content of life science to study the binding of metal ions and proteins. Turner et al designed a probe for the first time by using different classes of transcriptional regulatory proteins genetically and chemically modified to detect arsenic, hydroxylated polychlorinated biphenyls (OH-PCBs) and cyclic AMP (cAMP) [ 77 ]. In recent years, both synthetic probes and genetically encoded biosensors have been extensively developed.…”

Section: Biomolecule and Cell-based Probesmentioning

confidence: 99%

Research Progress in Fluorescent Probes for Arsenic Species

Qiu

2022

Molecules

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Arsenic is a toxic non-metallic element that is widely found in nature. In addition, arsenic and arsenic compounds are included in the list of Group I carcinogens and toxic water pollutants. Therefore, rapid and efficient methods for detecting arsenic are necessary. In the past decade, a variety of small molecule fluorescent probes have been developed, which has been widely recognized for their rapidness, efficiency, convenience and sensitivity. With the development of new nanomaterials (AuNPs, CDs and QDs), organic molecules and biomolecules, the conventional detection of arsenic species based on fluorescence spectroscopy is gradually transforming from the laboratory to the portable kit. Therefore, in view of the current research status, this review introduces the research progress of both traditional and newly developed fluorescence spectrometry based on novel materials for arsenic detection, and discusses the potential of this technology in the rapid screening and field testing of water samples contaminated with arsenic. The review also discusses the problems that still exist in this field, as well as the expectations.

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Section: Biomolecule and Cell-based Probesmentioning

confidence: 99%

Research Progress in Fluorescent Probes for Arsenic Species

Qiu

2022

Molecules

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“…13 Although these enzymes were effective, they lacked selectivity as they are highly promiscuous and thus, produce a high background due to the signal generated from a plethora of false positives. [14][15][16] Recently, the nitrogen regulatory protein C (NtrC) family of proteins such as phenol regulatory protein -MopR, 17 2-hydroxybiphenyl regulatory protein -HbpR, 18 and dimethyl phenol regulatory protein -DmpR 19 has been exploited as alternative enzyme systems. Owing to their evolutionarily optimized sensor scaffold, they are apt for selective sensing of a specific pollutant.…”

Section: Introductionmentioning

confidence: 99%

Porous silica nanospheres: a nanoplatform towards protein immobilization and cogent design of biosensors for aromatic water pollutant detection

Das,

Sahu,

Bandyopadhyaya

et al. 2023

Environ. Sci.: Nano

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“…Similar to aptamers, LBDs frequently couple ligand binding to structural switching, making them an ideal tool for the construction of reagentless single molecule sensors . Although different classes of LBDs such as transcription factors were exploited for the detection of LMW compounds, up to now most studies have focused on bacterial periplasmic binding proteins . These efforts resulted in an electrochemical sensor for maltose quantification, single molecule FRET sensors for in vivo imaging, as well as variants modified with fluorescent dyes for the detection of various sugars, ions and amino acids. , Fluorescent periplasmic binding proteins were integrated into devices for continuous monitoring of glucose concentrations and entered clinical studies for applications inside the human body .…”

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confidence: 99%

Structure-Based Design of Versatile Biosensors for Small Molecules Based on the PAS Domain of a Thermophilic Histidine Kinase

2018

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The development of biosensors for in vitro quantification of small molecules such as metabolites or manmade chemicals is still a major challenge. Here we show that engineered variants of the sensory PAS domain of the histidine kinase CitA of the thermophilic bacterium Geobacillus thermoleovorans represent promising alternatives to established biorecognition elements. By combining binding site grafting and rational design we constructed protein variants binding L-malate, ethylmalonate, or the aromatic compound phthalate instead of the native ligand citrate. Due to more favorable entropy contributions, the wild-type protein and its engineered variants exhibited increased (nano-to micromolar) affinities and improved enantioselectivity compared to CitA homologues of mesophilic organisms. Ligand binding was directly converted into an optical signal that was preserved after immobilization of the protein. A fluorescently labeled variant was used to quantify ethylmalonate, an urinary biomarker for ethylmalonic encephalopathy, in synthetic urine, thereby demonstrating the applicability of the sensor in complex samples.

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Transcriptional regulatory proteins as biosensing tools

Cited by 4 publications

References 40 publications

Research Progress in Fluorescent Probes for Arsenic Species

Research Progress in Fluorescent Probes for Arsenic Species

Porous silica nanospheres: a nanoplatform towards protein immobilization and cogent design of biosensors for aromatic water pollutant detection

Structure-Based Design of Versatile Biosensors for Small Molecules Based on the PAS Domain of a Thermophilic Histidine Kinase

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