Transcriptional repression of light-induced flavonoid synthesis by elicitor treatment of cultured parsley cells

Lozoya, Edmundo; Block, Annette; Lois, Rodrigo; Hahlbrock, Klaus; Scheel, Dierk

doi:10.1046/j.1365-313x.1991.00227.x

Cited by 17 publications

(30 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its decline upon infection is in line with both this functional assignment and the previous observation in parsley that the response to UV irradiation is strongly overridden by treatment with a pathogen-derived elicitor (53). However, as this latter effect includes transcriptional repression of previously UV light-induced chalcone synthase, that is, of all branches of flavonoid biosynthesis, it is surprising that the two kaempferol glycosides A and B remained unaltered under the present conditions.…”

Section: Discussionsupporting

confidence: 90%

Accumulation of soluble and wall-bound indolic metabolites in Arabidopsis thaliana leaves infected with virulent or avirulent Pseudomonas syringae pathovar tomato strains

Hagemeier¹

2001

Proceedings of the National Academy of Sciences

View full text Add to dashboard Cite

The chemical structures and accumulation kinetics of several major soluble as well as wall-bound, alkali-hydrolyzable compounds induced upon infection of Arabidopsis thaliana leaves with Pseudomonas syringae pathovar tomato were established. All identified accumulating products were structurally related to tryptophan. Most prominent among the soluble substances were tryptophan, ␤-D-glucopyranosyl indole-3-carboxylic acid, 6-hydroxyindole-3-carboxylic acid 6-O-␤-D-glucopyranoside, and the indolic phytoalexin camalexin. The single major accumulating wall component detectable under these conditions was indole-3-carboxylic acid. All of these compounds increased more rapidly, and camalexin as well as indole-3-carboxylic acid reached much higher levels, in the incompatible than in the compatible P. syringae͞A. thaliana interaction. The only three prominent phenylpropanoid derivatives present in the soluble extract behaved differently. Two kaempferol glycosides remained largely unaffected, and sinapoyl malate decreased strongly upon bacterial infection with a time course inversely correlated with that of the accumulating tryptophan-related products. The accumulation patterns of both soluble and wallbound compounds, as well as the disease resistance phenotypes, were essentially the same for infected wild-type and tt4 (no kaempferol glycosides) or fah1 (no sinapoyl malate) mutant plants. Largely different product combinations accumulated in wounded or senescing A. thaliana leaves. It seems unlikely that any one of the infection-induced compounds identified so far has a decisive role in the resistance response to P. syringae.

show abstract

Section: Discussionsupporting

confidence: 90%

Accumulation of soluble and wall-bound indolic metabolites in Arabidopsis thaliana leaves infected with virulent or avirulent Pseudomonas syringae pathovar tomato strains

Hagemeier¹

2001

Proceedings of the National Academy of Sciences

View full text Add to dashboard Cite

show abstract

“…Exposure to different microbes or MAMPs leads to repression of flavonoid accumulation in plants (8,9), at the cost of the adaptation to various abiotic stresses (21). For example, sucrose stress-induced anthocyanin accumulation is blocked in the presence of flg22 or elf18 in Arabidopsis seedlings ( Fig.…”

Section: Identification Of Arabidopsis Priority In Sweet Life (Psl) Mmentioning

confidence: 99%

“…Because prolonged defense activation results in growth retardation, repression of abiotic stress responses, and/or cell death in plants (5,(7)(8)(9), plants evolved specific mechanisms conferring stringent control on abundance and activation/deactivation states of immune receptors. As for intracellular immune receptors containing nucleotide binding and leucine-rich repeat (LRR) domains, a widespread class of disease resistance proteins, the importance of protein abundance control via cytosolic HSP90/SGT1/RAR1 chaperone complexes has been well documented (10).…”

mentioning

confidence: 99%

Uncoupling of sustained MAMP receptor signaling from early outputs in an Arabidopsis endoplasmic reticulum glucosidase II allele

Tintor

Mentzel

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

121

103

View full text Add to dashboard Cite

Recognition of microbe-associated molecular patterns (MAMPs), conserved structures typical of a microbial class, triggers immune responses in eukaryotes. This is accompanied by a diverse set of physiological responses that are thought to enhance defense activity in plants. However, the extent and mechanisms by which MAMPinduced events contribute to host immunity are poorly understood. Here we reveal Arabidopsis priority in sweet life4 (psl4) and psl5 mutants that are insensitive to the bacterial elongation factor (EF)-Tu epitope elf18 but responsive to flagellin epitope flg22. PSL4 and PSL5, respectively, identify ␤-and ␣-subunits of endoplasmic reticulumresident glucosidase II, which is essential for stable accumulation and quality control of the elf18 receptor EFR but not the flg22 receptor FLS2. We notice that EFR signaling is partially and differentially impaired without a significant decrease of the receptor steady-state levels in 2 weakly dysfunctional gII␣ alleles, designated psl5-1 and rsw3. Remarkably, rsw3 plants exhibit marked supersusceptibility against a virulent bacterial phytopathogen despite nearly intact coactivation of MAPKs, reactive oxygen species, ethylene biosynthesis, and callose deposition in response to elf18, demonstrating that these signaling outputs alone are insufficient to mount effective immunity. However, rsw3 plants fail to maintain high transcript levels of defense-promoting WRKY, PR1, and PR2 genes at late time points (4 to 24 h) after elf18 elicitation. This points to an unexpected separation between initial and sustained activation of EFR-mediated signaling in the absence of proper glucosidase II-mediated endoplasmic reticulum quality control. Our findings strongly suggest the importance of sustained MAMP receptor signaling as a key step in the establishment of robust immunity.EFR ͉ ER quality control ͉ LRR RLK ͉ plant immunity

show abstract

“…Hence, the free hydroxycinnamoyl seems to prevent fungal degradation of the plant cell wall rather than to exert a direct fungi-toxic activity [6]. In cultured parsley cells, treatment with a fungal elicitor or exposure to UV-containing white light stimulates partly overlapping phenylpropanoid biosynthetic pathways [35]. Elicitor treatment induces a branch pathway that leads to the secretion of antimicrobial furanocoumarins, whereas UV light stimulates a different branch pathway that leads to the accumulation of flavonoids in vacuoles [35].…”

mentioning

confidence: 99%

“…In cultured parsley cells, treatment with a fungal elicitor or exposure to UV-containing white light stimulates partly overlapping phenylpropanoid biosynthetic pathways [35]. Elicitor treatment induces a branch pathway that leads to the secretion of antimicrobial furanocoumarins, whereas UV light stimulates a different branch pathway that leads to the accumulation of flavonoids in vacuoles [35]. It should be interesting to find out whether these examples of pathogen-triggered secretion events are based on a common molecular framework, which might include components that are the same or similar to those in SNARE-dependent resistance to powdery mildews at the cell periphery [21 ].…”

mentioning

confidence: 99%

Knocking on the heaven’s wall: pathogenesis of and resistance to biotrophic fungi at the cell wall

Schulze‐Lefert

2004

Current Opinion in Plant Biology

124

101

View full text Add to dashboard Cite

New findings challenge the traditional view of the plant cell wall as passive structural barrier to invasion by fungal microorganisms. A surveillance system for cell wall integrity appears to sense perturbation of the cell wall structure upon fungal attack and is interconnected with known plant defence signalling pathways. Biotrophic fungi might manipulate this surveillance system for the establishment of biotrophy. The attempts of fungi to invade also induce a sub-cellular polarisation in attacked cells, which activates an ancient vesicle-associated resistance response that possibly enables the focal transport of regulatory cargo and the secretion of toxic cargo. The underlying resistance machinery might have been subverted by biotrophic fungi for pathogenesis. IntroductionPenetration through the plant cell wall represents an Achilles heel in the pathogenesis of most biotrophic fungi and marks a lifestyle transition from extra-cellular to invasive growth. Modification of the plant cell wall was recognised for the first time as potential resistance mechanism almost 80 years ago following work in which 78 plant species and varieties were challenged with Alternaria spp. and other leaf-spotting fungi [1]. The termination of fungal pathogenesis at the cell wall was commonly associated with wall 'thickenings' and the formation of local additions or 'callosities' in the paramural space (i.e. the space between the cell wall and the plasma membrane). Formation of these cell wall appositions (CWAs) or papillae is usually accompanied by a co-localised accumulation of phenolics and reactive oxygen species [2][3][4][5][6]. The complex process of sub-cellular cell wall remodelling is tightly linked to the rapid disassembly and subsequent focal reassembly of the plant cytoskeleton at fungal entry sites, which is indicative of a pathogen-triggered cell polarisation [6][7][8][9]. There has been a long-standing controversy, however, over whether CWAs function in disease resistance or facilitate the entry of fungal pathogens into host cells by providing a structural collar for the intruder. New molecular genetic data from Arabidopsis and barley have indeed revealed that molecular processes at and in CWAs have Janus-faced functions, that is, functions for fungal pathogenesis and in resistance responses. Focal vesicle transport and vesicle fusion events that are dependent on SNAP (soluble N-ethylmaleimide-sensitive-factor-association protein) receptor (SNARE) proteins at the plasma membrane emerge as potential common underlying mechanisms that might be interconnected with a poorly understood cell wall integrity surveillance system. In this review, I discuss the seemingly paradoxical functions of these processes in establishing the biotrophic lifestyle and in disease resistance at the cell periphery.Linking cell wall structure to biotic stress signalling Callose, a (1!3)-b-D-glucan, has long been known to be synthesised and deposited rapidly at CWAs upon microbial attack. This polymer was thought to contribute to a physical barrie...

show abstract

Transcriptional repression of light-induced flavonoid synthesis by elicitor treatment of cultured parsley cells

Cited by 17 publications

References 0 publications

Accumulation of soluble and wall-bound indolic metabolites in Arabidopsis thaliana leaves infected with virulent or avirulent Pseudomonas syringae pathovar tomato strains

Accumulation of soluble and wall-bound indolic metabolites in Arabidopsis thaliana leaves infected with virulent or avirulent Pseudomonas syringae pathovar tomato strains

Uncoupling of sustained MAMP receptor signaling from early outputs in an Arabidopsis endoplasmic reticulum glucosidase II allele

Knocking on the heaven’s wall: pathogenesis of and resistance to biotrophic fungi at the cell wall

Contact Info

Product

Resources

About