Transcriptional Responses of Escherichia coli K-12 and O157:H7 Associated with Lettuce Leaves

Fink, Ryan C.; Black, Elaine P.; Hou, Zhe; Sugawara, Masayuki; Sadowsky, Michael J.; Diez-Gonzalez, Francisco

doi:10.1128/aem.00787-12

Cited by 20 publications

(39 citation statements)

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“…According to previous research, biofilm was a major exist form of E. coli O157:H7 bacteria on fresh produce, such as lettuce, cucumber, spinach (Fink et al . ; Macarisin et al . ).…”

Section: Discussionmentioning

confidence: 99%

Essential Oils-Based Antibacterial Agent Against Escherichia coli O157:H7 Biofilm on Cucumber

Cui

Zhao

Li³

et al. 2016

Journal of Food Processing and Preservation

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Guided by the principle of hurdle technology, a high‐efficiency antibacterial agent (AA) had been formulated, which contained six kinds of essential oils, three pieces of food additives and ethyl alcohol. The antibacterial effects of AA against E. coli O157:H7 biofilm on simulated and real cucumbers were testified by MTT staining method, colony‐forming unit (CFU) counting method, scanning electron microscopy (SEM) analysis and confocal laser scanning microscopy (CLSM) assay. The remarkable anti‐biofilm activity of AA was achieved under low concentration and short treatment duration. The results also revealed that AA inhibited the multiplication of E. coli O157:H7 via destroying the cell membrane integrity. Besides, color, texture and sensory evaluation illustrated that AA could be applied as a disinfectant to eliminate the biofilm formed on cucumber without impact on food quality. Practical Applications As a kind of high‐efficiency antimicrobials, AA has significant inhibition and elimination effects on biofilm under a low concentration and short incubation time. Especially, AA was effective to eliminate the formed E. coli O157:H7 biofilm on the surface of cucumber without impact on the quality and flavor of the product. On the basis of the results, we believe that AA can be applied in vegetables or fruits processing and preservation.

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“…According to previous research, biofilm was a major exist form of E. coli O157:H7 bacteria on fresh produce, such as lettuce, cucumber, spinach (Fink et al . ; Macarisin et al . ).…”

Section: Discussionmentioning

confidence: 99%

Essential Oils-Based Antibacterial Agent Against Escherichia coli O157:H7 Biofilm on Cucumber

Cui

Zhao

Li³

et al. 2016

Journal of Food Processing and Preservation

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“…So far, one microarray study analysed the transcriptome of S. Typhimurium SL1344 grown on cilantro leaves which was co‐inoculated with Dickeya dadantii , a plant macerating pathogen (Goudeau et al ., ) that showed a shift towards anaerobic metabolism. In two other microarray studies, transcriptome analyses of E. coli O157:H7 on vegetables have been reported (Kyle et al ., ; Fink et al ., ). The first studied the response of E. coli O157:H7 to lettuce lysate, resulting in strong oxidative stress of the bacterium.…”

Section: Discussionmentioning

confidence: 97%

“…The first studied the response of E. coli O157:H7 to lettuce lysate, resulting in strong oxidative stress of the bacterium. In the second recently published study, gene expression of E. coli O157:H7 on lettuce leafs was determined representing the first transcriptomic analysis of this pathogen on intact cell material (Fink et al ., ). Both studies show similarities to our work, but gene expression patterns varied.…”

Section: Discussionmentioning

confidence: 99%

“…In former studies, it was shown that curli and long aggregative fimbriae, which also were found to mediate binding to epithelial cells, were transcribed during attachment of E. coli O157:H7 to salad and of Salmonella spp. to alfalfa sprouts (Barak et al ., ; Fink et al ., ). In our study, the csgDEFG operon and csgCAB ( agfDEFG and agfCAB equivalent) were transcribed in both media with csgB, csgG and csgD being more transcribed in presence of sprouts.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptional profile of Salmonella enterica subsp. enterica serovar Weltevreden during alfalfa sprout colonization

Brankatschk

Kamber

Pothier

et al. 2013

Microbial Biotechnology

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Sprouted seeds represent a great risk for infection by human enteric pathogens because of favourable growth conditions for pathogens during their germination. The aim of this study was to identify mechanisms of interactions of Salmonella enterica subsp. enterica Weltevreden with alfalfa sprouts. RNA-seq analysis of S. Weltevreden grown with sprouts in comparison with M9-glucose medium showed that among a total of 4158 annotated coding sequences, 177 genes (4.3%) and 345 genes (8.3%) were transcribed at higher levels with sprouts and in minimal medium respectively. Genes that were higher transcribed with sprouts are coding for proteins involved in mechanisms known to be important for attachment, motility and biofilm formation. Besides gene expression required for phenotypic adaption, genes involved in sulphate acquisition were higher transcribed, suggesting that the surface on alfalfa sprouts may be poor in sulphate. Genes encoding structural and effector proteins of Salmonella pathogenicity island 2, involved in survival within macrophages during infection of animal tissue, were higher transcribed with sprouts possibly as a response to environmental conditions. This study provides insight on additional mechanisms that may be important for pathogen interactions with sprouts.

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“…Curli, in particular, have been implicated in the initial adhesion phase which leads to biofilm formation in several strains of Escherichia coli (Fink et al, 2012;Lee et al, 2011). In most E. coli the biofilm master regulator csgD is critical for modulating curli production and serves as a hub for integrating various extracellular signals, thereby dictating whether or not the bacteria initiate biofilm formation (Ogasawara et al, 2010(Ogasawara et al, , 2011Pesavento et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

A Q-like transcription factor regulates biofilm development in Escherichia coli by controlling expression of the DLP12 lysis cassette

et al. 2013

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The DLP12 lysis cassette (essD, ybcT, rzpD/rzoD) is required in certain Escherichia coli strains for normal curli expression and biofilm development. Tightly controlled regulation of the lysis cassette is of particular importance, since its overexpression causes host cell lysis. In silico analysis revealed a putative intrinsic transcriptional terminator 100 bp upstream of essD and within 2000 bp of ybcQ (Q DLP12 ), a putative lambda (l) Q-like antiterminator. We hypothesized that Q DLP12 may be required for effective expression of the lysis cassette. In this work we report on the role of Q DLP12 as a positive regulator of DLP12 lysis cassette expression. Mutants lacking Q DLP12 exhibited a biofilm-defective phenotype analogous to that of the lysis cassette knockouts.This defect occurred through the downregulation of curli transcription, which is also consistent with that seen in the lysis cassette mutants and was restored by complementation by ectopic expression of Q DLP12 . In addition, Q DLP12 overexpression caused cell lysis, as demonstrated by leakage of b-galactosidase activity from cells. This was accompanied by upregulation of the DLP12 lysis cassette as demonstrated by increased essD transcription, which was documented with gfp-reporter assays, RT-PCR and chromatin immunoprecipitation (ChIP). We provide evidence that this Q-mediated effect resulted from direct interaction of Q DLP12 with the lysis cassette promoter (essDp), as demonstrated by electrophoretic gel mobility shift assay (EMSA). We propose that Q DLP12 encodes a functional transcriptional regulator, which promotes expression of the DLP12 lysis cassette. This work provides evidence of a regulator from a defective prophage affecting host cell physiology.

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Transcriptional Responses of Escherichia coli K-12 and O157:H7 Associated with Lettuce Leaves

Cited by 20 publications

References 0 publications

Essential Oils-Based Antibacterial Agent Against Escherichia coli O157:H7 Biofilm on Cucumber

Essential Oils-Based Antibacterial Agent Against Escherichia coli O157:H7 Biofilm on Cucumber

Transcriptional profile of Salmonella enterica subsp. enterica serovar Weltevreden during alfalfa sprout colonization

A Q-like transcription factor regulates biofilm development in Escherichia coli by controlling expression of the DLP12 lysis cassette

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