Transcriptome analysis and identification of genes associated with chicken sperm storage duration

Yang, Liubin; Zheng, Xin; Mo, Changhuan; Li, Shaomei; Liu, Zhiwei; Yang, Ge; Zhao, Qianqian; Li, Shijun; Mou, Chunyan

doi:10.1016/j.psj.2019.10.021

Cited by 26 publications

(19 citation statements)

References 40 publications

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“…After removal of low-quality reads, there were average 79.63% reads mapped to the reference genome. This was similar to that of other studies on chicken transcriptome, such as 73.1% ( Liu et al, 2018 ) and 80.2% ( Xing et al, 2020 ) in testis, 82% in sperm storage tubules ( Yang et al, 2020 ), and 75.73% in intraepithelial lymphocyte natural killer cells ( Boo et al, 2020 ). In our previous studies, screening candidate genes associated with sperm motility in testis of chickens with divergent sperm motility by RNA-seq was performed ( Xing et al, 2020 ), and 3 DEGs ([matrix metalloproteinase-9] MMP9 , [AHNAK nucleoprotein 2] AHNAK2 , and [sarcolipin] SLN ) were identified.…”

Section: Disscussionsupporting

confidence: 91%

Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters

et al. 2022

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Sperm motility is a crucial trait in chicken production, and the epididymis is an essential organ in the reproductive system. Currently, the molecular mechanisms underlying sperm motility in the epididymis are unclear. In this study, 8 cDNA libraries and eight miRNA libraries were constructed from roosters (4 chickens per group) with diverse sperm motility. After a comparative analysis of epididymal transcriptomes, we detected 84 differentially expressed genes ( DEG s) using the edgeR package. Integrated interpretation of DEGs indicated that MMP9, SLN, WT1, PLIN1 , and LRRIQ1 are the most promising candidate genes affecting sperm motility in the epididymis of roosters. MiR-146a, mir-135b, and mir-205 could play important regulatory roles in sperm maturation, capacitation, and motility. Additionally, a comprehensive analysis of the mRNA and miRNAs transcriptomes in silico identified a promising gene-miRNA pair miR-135b-HPS5, which may be a vital regulator of sperm motility in the epididymis. Our findings provide novel integrated information of miRNAs and genes that shed light on the regulatory mechanisms of fertility in roosters.

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Section: Disscussionsupporting

confidence: 91%

Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters

et al. 2022

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“…The protein ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3GAL1) was highly enriched in good stallions. This protein plays a role in posttranscriptional modification of proteins, and interestingly the gene for ST3GAL1 has previously been associated with sperm storage duration in the oviduct in chickens [55]. This finding falls in line with the special dependence of post transcriptional modifications for the proper functioning of the spermatozoa.…”

Section: Discussionmentioning

confidence: 77%

Seminal plasma AnnexinA2 protein is a relevant biomarker for stallions which require removal of seminal plasma for sperm survival upon refrigeration†

Gaitskell‐Phillips

Martín-Cano

Ortiz-Rodríguez

et al. 2020

Biology of Reproduction

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Some stallions yield ejaculates which do not tolerate conservation by refrigeration prior to artificial insemination (AI), showing improvement after removal of most of the seminal plasma (SP) by centrifugation. In this study, the SP-proteome of 10 different stallions was defined through HPLC/MS/MS and bioinformatic analysis in relation to the ability of the ejaculates to maintain semen quality when cooled and stored at 5 °C. Stallions were classified into three groups, depending on this ability: those maintaining good quality after direct extension in a commercial extender (good), stallions requiring removal of seminal plasma (RSP) to maintain seminal quality (good-RSP), and stallions, unable to maintain good semen quality even after RSP (poor). Pathway enrichment analysis of the proteins identified in whole equine seminal plasma using human orthologs was performed using g: profiler showing enriched Reactome and KEGG pathways related to hexose metabolism, vesicle mediated transport, post translational modification of proteins and immune response. Specific proteins overrepresented in stallions tolerating conservation by refrigeration included a peroxiredoxin-6 like protein, and transcobalamin-2, a primary vitamin B12-binding and transport protein. Also, the protein involved in protein glycosylation, ST3 beta-galactoside alpha-2,3-sialyltransferase 1 was present in good stallions. These proteins were nearly absent in poor stallions. Particularly, annexinA2 appeared as to be the most powerful discriminant variable for identification of stallions needing seminal plasma-removal prior to refrigeration, with a p = 0.002 and a q value = 0.005. Overall this is the first detailed study of the equine SP-proteome, showing the potential value of specific proteins as discriminant bio-markers for clinical classification of stallions for AI.

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“…Conventional transcriptome sequencing analysis has been widely used in the study of various traits of poultry ( Luo et al, 2019 ; Yang et al, 2020 ). In this study, we used basic differential expression analysis and gene coexpression network analysis to identify molecular network pathways that are involved in the regulation of glycogen content in chicken breast muscle.…”

Section: Discussionmentioning

confidence: 99%

FOSL2 Is Involved in the Regulation of Glycogen Content in Chicken Breast Muscle Tissue

Liu

Liu²,

Wang³

et al. 2021

Front. Physiol.

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The glycogen content in muscle of livestock and poultry animals affects the homeostasis of their body, growth performance, and meat quality after slaughter. FOS-like 2, AP-1 transcription factor subunit (FOSL2) was identified as a candidate gene related to muscle glycogen (MG) content in chicken in our previous study, but the role of FOSL2 in the regulation of MG content remains to be elucidated. Differential gene expression analysis and weighted gene coexpression network analysis (WGCNA) were performed on differentially expressed genes (DEGs) in breast muscle tissues from the high-MG-content (HMG) group and low-MG-content (LMG) group of Jingxing yellow chickens. Analysis of the 1,171 DEGs (LMG vs. HMG) identified, besides FOSL2, some additional genes related to MG metabolism pathway, namely PRKAG3, CEBPB, FOXO1, AMPK, and PIK3CB. Additionally, WGCNA revealed that FOSL2, CEBPB, MAP3K14, SLC2A14, PPP2CA, SLC38A2, PPP2R5E, and other genes related to the classical glycogen metabolism in the same coexpressed module are associated with MG content. Also, besides finding that FOSL2 expression is negatively correlated with MG content, a possible interaction between FOSL2 and CEBPB was predicted using the STRING (Search Tool for the Retrieval of Interacting Genes) database. Furthermore, we investigated the effects of lentiviral overexpression of FOSL2 on the regulation of the glycogen content in vitro, and the result indicated that FOSL2 decreases the glycogen content in DF1 cells. Collectively, our results confirm that FOSL2 has a key role in the regulation of the MG content in chicken. This finding is helpful to understand the mechanism of MG metabolism regulation in chicken and provides a new perspective for the production of high-quality broiler and the development of a comprehensive nutritional control strategy.

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Transcriptome analysis and identification of genes associated with chicken sperm storage duration

Cited by 26 publications

References 40 publications

Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters

Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters

Seminal plasma AnnexinA2 protein is a relevant biomarker for stallions which require removal of seminal plasma for sperm survival upon refrigeration†

FOSL2 Is Involved in the Regulation of Glycogen Content in Chicken Breast Muscle Tissue

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