Transcriptome Analysis of a Multiple-Branches Mutant Terminal Buds in Betula platyphylla × B. pendula

Han, Rui; Wang, Shuo; Liu, Chaoyi; Xu, Wendi; Bian, Xiuyan; Jiang, Jing

doi:10.3390/f10050374

Cited by 3 publications

(4 citation statements)

References 79 publications

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“…The study results showed that the mutant plant was characterized by multiple branches, short branches, and short leaves, while the wild-type plant was characterized by few branches, long branches, and long leaves. Similar results were also reported in few other plants, such as physic nut (Chen et al, 2019) and birch (Han et al, 2019). In addition, the observational and statistical analyses of mutant grafted seedlings showed that the variant traits could be retained in grafted plants.…”

Section: Changes In Morphological and Cytological Structures Of Mutant Plantssupporting

confidence: 85%

“…Cell division, expansion, and differentiation affect the fundamental processes of plant organ growth and development and affect the plant phenotypes (Sugiyama, 2005;Yang et al, 2015). In addition, the shoot apical meristem (SAM) is essential for the development of plants and is responsible for the development of leaves, stems, and flowers (Han et al, 2019). Therefore, we analyzed 23 DEGs related to cell division, differentiation, and SAM activity to further study the differences between wild-type and mutant plants (Figure 5A).…”

Section: Degs Involved In Cell Division Cell Differentiation and Sam Activitymentioning

confidence: 99%

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Insight Into the Multiple Branches Traits of a Mutant in Larix olgensis by Morphological, Cytological, and Transcriptional Analyses

Cai

Zhou

et al. 2021

Front. Plant Sci.

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Larix olgensis is a tall deciduous tree species that has many applications in the wood fiber industry. Bud mutations are somatic mutations in plants and are considered an ideal material to identify and describe the molecular mechanism of plant mutation. However, the molecular regulatory mechanisms of bud mutations in L. olgensis remain unknown. In this study, dwarfed (or stunted), short-leaved, and multi-branched mutants of L. olgensis were found and utilized to identify crucial genes and regulatory networks controlling the multiple branch structure of L. olgensis. The physiological data showed that the branch number, bud number, fresh and dry weight, tracheid length, tracheid length-width ratio, inner tracheid diameter, and epidermal cell area of mutant plants were higher than that of wild-type plants. Hormone concentration measurements found that auxin, gibberellin, and abscisic acid in the mutant leaves were higher than that in wild-type plants. Moreover, the transcriptome sequencing of all samples using the Illumina Hiseq sequencing platform. Transcriptome analysis identified, respectively, 632, 157, and 199 differentially expressed genes (DEGs) in buds, leaves, and stems between mutant plants and wild type. DEGs were found to be involved in cell division and differentiation, shoot apical meristem activity, plant hormone biosynthesis, and sugar metabolism. Furthermore, bZIP, WRKY, and AP2/ERF family transcription factors play a role in bud formation. This study provides new insights into the molecular mechanisms of L. olgensis bud and branch formation and establishes a fundamental understanding of the breeding of new varieties in L. olgensis.

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Section: Changes In Morphological and Cytological Structures Of Mutant Plantssupporting

confidence: 85%

Section: Degs Involved In Cell Division Cell Differentiation and Sam Activitymentioning

confidence: 99%

Insight Into the Multiple Branches Traits of a Mutant in Larix olgensis by Morphological, Cytological, and Transcriptional Analyses

Cai

Zhou

et al. 2021

Front. Plant Sci.

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“…Compared with MP, OP developed more branches at an early stage and the leaf axillary buds produced spiny spur shoots. Similar results have been reported in other species, such as birch and larch ( Han et al., 2019 ; Cai et al., 2021 ). In addition, the vascular bundles and abaxial epidermal cells of MP leaves were larger than those of OP, and the areal proportion of the pith, xylem, and phloem in new shoots of MP from the base to the tip was greater than that of OP, and almost no conducting tissue had differentiated at the shoot tip of OP.…”

Section: Discussionsupporting

confidence: 91%

“…The meristem cells of buds undergo genetic changes during cell division, leading to the development of lateral branches ( Leng et al., 2021 ). The shoot apical meristem (SAM) is also crucial to plant development and is responsible for the development of leaves, stems, and flowers ( Han et al., 2019 ). The SAM gives rise to leaf primordia during the vegetative growth stage and an inflorescence meristem in the transitional stage from vegetative growth to reproductive growth.…”

Section: Introductionmentioning

confidence: 99%

Jasmonic acid negatively regulates branch growth in pear

Cheng

Liang²,

Qiu³

et al. 2023

Front. Plant Sci.

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The quality of seedlings is an important factor for development of the pear industry. A strong seedling with few branches and suitable internodes is ideal material as a rootstock for grafting and breeding. Several branching mutants of pear rootstocks were identified previously. In the present study, ‘QAU-D03’ (Pyrus communis L.) and it’s mutants were used to explore the mechanism that affects branch formation by conducting phenotypic trait assessment, hormone content analysis, and transcriptome analysis. The mutant plant (MP) showed fewer branches, shorter 1-year-old shoots, and longer petiole length, compared to original plants (OP), i.e., wild type. Endogenous hormone analysis revealed that auxin, cytokinin, and jasmonic acid contents in the stem tips of MP were significantly higher than those of the original plants. In particular, the jasmonic acid content of the MP was 1.8 times higher than that of the original plants. Transcriptome analysis revealed that PcCOI1, which is a transcriptional regulatory gene downstream of the jasmonic acid signaling pathway, was expressed more highly in the MP than in the original plants, whereas the expression levels of PcJAZ and PcMYC were reduced in the MP compared with that of the original plants. In response to treatment with exogenous methyl jasmonate, the original plants phenotype was consistent with that of the MP in developing less branches. These results indicate that jasmonic acid negatively regulates branch growth of pear trees and that jasmonic acid downstream regulatory genes play a crucial role in regulating branching.

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Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla × Betula pendula

Han

et al. 2019

BMC Plant Biol

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BackgroundPlant architecture, which is mostly determined by shoot branching, plays an important role in plant growth and development. Thus, it is essential to explore the regulatory molecular mechanism of branching patterns based on the economic and ecological importance. In our previous work, a multiple-branches birch mutant br was identified from 19 CINNAMOYL-COENZYME A REDUCTASE 1 (CCR1)-overexpressed transgenic lines, and the expression patterns of differentially expressed genes in br were analyzed. In this study, we further explored some other characteristics of br, including plant architecture, wood properties, photosynthetic characteristics, and IAA and Zeatin contents. Meanwhile, the T-DNA insertion sites caused by the insertion of exogenous BpCCR1 in br were identified to explain the causes of the mutation phenotypes.ResultsThe mutant br exhibited slower growth, more abundant and weaker branches, and lower wood basic density and lignin content than BpCCR1 transgenic line (OE2) and wild type (WT). Compared to WT and OE2, br had high stomatal conductance (Gs), transpiration rate (Tr), but a low non-photochemical quenching coefficient (NPQ) and chlorophyll content. In addition, br displayed an equal IAA and Zeatin content ratio of main branches’ apical buds to lateral branches’ apical buds and high ratio of Zeatin to IAA content. Two T-DNA insertion sites caused by the insertion of exogenous BpCCR1 in br genome were found. On one site, chromosome 2 (Chr2), no known gene was detected on the flanking sequence. The other site was on Chr5, with an insertion of 388 bp T-DNA sequence, resulting in deletion of 107 bp 5′ untranslated region (UTR) and 264 bp coding sequence (CDS) on CORONATINE INSENSITIVE 1 (BpCOII). In comparison with OE2 and WT, BpCOI1 was down-regulated in br, and the sensitivity of br to Methyl Jasmonate (MeJA) was abnormal.ConclusionsPlant architecture, wood properties, photosynthetic characteristics, and IAA and Zeatin contents in main and lateral branches’ apical buds changed in br over the study’s time period. One T-DNA insertion was identified on the first exon of BpCOI1, which resulted in the reduction of BpCOI1 expression and abnormal perception to MeJA in br. These mutation phenotypes might be associated with a partial loss of BpCOI1 in birch.

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Transcriptome Analysis of a Multiple-Branches Mutant Terminal Buds in Betula platyphylla × B. pendula

Cited by 3 publications

References 79 publications

Insight Into the Multiple Branches Traits of a Mutant in Larix olgensis by Morphological, Cytological, and Transcriptional Analyses

Insight Into the Multiple Branches Traits of a Mutant in Larix olgensis by Morphological, Cytological, and Transcriptional Analyses

Jasmonic acid negatively regulates branch growth in pear

Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla × Betula pendula

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