Transcriptome analysis of Anopheles dirus and Plasmodium vivax at ookinete and oocyst stages

Boonkaew, Tippawan; Mongkol, W.; Prasert, Sureerat; Paochan, Pattaweeya; Yoneda, Saki; Nguitragool, Wang; Kumpitak, Chalermpon; Sattabongkot, Jetsumon; Kubera, Anchanee

doi:10.1016/j.actatropica.2020.105502

Cited by 11 publications

(18 citation statements)

References 46 publications

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“…On the contrary, our data with P. vivax infected mosquitoes is in good agreement with results obtained with A. aquasalis (Santana et al 2019) and An. dirus (Boonkaew et al 2020), despite these studies used well established mosquito colonies. While the An.…”

Section: Discussionmentioning

confidence: 99%

“…With the advances of Next Generation Sequencing (NGS) technologies, transcriptomic approaches are being developed to gain further insights into Anopheles responses to P. vivax (Santana et al 2019;Boonkaew et al 2020;Kumari et al 2021). Although providing substantial novel data, these studies were conducted with local Anopheles vectors for which limited molecular information or genetic tools are available and did not address the specificity of the mosquito response to either human Plasmodium species.…”

Section: Introductionmentioning

confidence: 99%

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Differential transcriptomic response ofAnopheles arabiensistoPlasmodium vivaxandPlasmodium falciparuminfection

Kornobis

Puchot

et al. 2021

Preprint

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Plasmodium vivax malaria is now recognized as the second most dangerous parasitic threat to human health with the regular decrease of Plasmodium falciparum worldwide over recent decades. A very limited numbers of studies address the interaction of P. vivax with its Anopheles mosquito vectors. Those studies were conducted in P. vivax endemic countries with P.vivax local major vectors for which limited genomic and genetic tools are available. Despite the presence of P. vivax in several African countries and increasing reports on its occurrence in many others, there is virtually no data on the molecular responses of Anopheles arabiensis, a major African mosquito vector, to P. vivax, which limits the development of further mosquito-targeted interventions aimed at reducing P. vivax transmission. Taking advantage of the situation of Madagascar where P. falciparum, P. vivax and An. arabiensis are present, we explore the molecular responses of An. arabiensis towards these two human malaria parasites. RNA sequencing on RNAs isolated from mosquito midguts dissected at the early stage of infection (24 hours) was performed using mosquitoes fed on the blood of P. vivax and P. falciparum gametocyte carriers in a field station. From a de novo assembly of An. arabiensis midgut total RNA transcriptome, the comparative analysis revealed that a greater number of genes were differentially expressed in the mosquito midgut in response to P. vivax (209) than to P. falciparum (81). Among these, 15 common genes were identified to be significantly expressed in mosquito midgut 24 hours after ingesting P. vivax and P. falciparum gametocytes, including immune responsive genes and genes involved in amino-acid detoxification pathways. Importantly, working with both wild mosquitoes and field circulating parasites, our analysis revealed a strong mosquito genotype by parasite genotype interaction. Our study also identified 51 putative long non-coding RNAs differentially expressed in An. arabiensis mosquito infected midgut. Among these, several mapped to the published An. arabiensis genome at genes coding immune responsive genes such as gambicin 1, leucine-rich repeat containing genes, either on sense or antisense strands. This study constitutes the first comparison of An. arabiensis molecular interaction with P. vivax and P. falciparum, investigating both coding and long non-coding RNAs for the identification of potential transcripts, that could lead to the development of novel approaches to simultaneously block the transmission of vivax and falciparum malaria.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential transcriptomic response ofAnopheles arabiensistoPlasmodium vivaxandPlasmodium falciparuminfection

Kornobis

Puchot

et al. 2021

Preprint

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show abstract

“…The blood pellet was resuspended in RPMI medium and centrifuged at 1008 g for 5 min. The blood pellet was resuspended again in na€ ıve AB serum and used for blood feeding at 5 µl for each mosquito [2]. The dsRNAtreated mosquitoes were dissected in phosphate-buffered saline (PBS) on the seventh day after the P. vivax challenge.…”

Section: Plasmodium Vivax Challenging and Oocyst Countingmentioning

confidence: 99%

“…dirus mosquitoes revealed that An. dirus yellow-g was significantly upregulated at 18 h after blood feeding [2]. The yellow-g gene was selected for this study to investigate its role in P. vivax development.…”

Section: Introductionmentioning

confidence: 99%

Anopheles dirus yellow‐g mediates Plasmodium vivax infection

Mongkol

Pomun

Nguitragool

et al. 2021

Tropical Med Int Health

Self Cite

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objective Our previous transcriptome analysis of Anopheles dirus revealed upregulation of the An. dirus yellow-g gene upon ingestion of Plasmodium vivax-infected blood. This gene belongs to the yellow gene family, but its role regarding P. vivax infection is not known and remains to be validated. The aim of this study was to investigate the role of the An. dirus yellow-g gene in P. vivax infection.methods The qRT-PCR was used to detect the expression of the yellow-g gene in many organs of both male and female mosquitos. The yellow-g gene silencing was performed by dsRNA membrane feeding to An. dirus. These mosquitoes were later challenged by P. vivax-infected blood. The oocyst numbers were determined.results The yellow-g transcript was detected in several organs of both male and female An. dirus mosquitoes. Successful knockdown of yellow-g was achieved and resulted in reduced P. vivax infection in the mosquitoes. The decrease in yellow-g expression had no effect on the life span of the mosquitoes. conclusions These results support the yellow-g gene as having an important function in Plasmodium development in Anopheles mosquitoes. keywords dsRNA, malaria, oocyst development, yellow-g Sustainable Development Goals: Good Health and Wellbeing *Contributed equally.

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“…Limited research has been undertaken on the underlying mechanism of P. vivax oocyst development (transition from a small oocyst of 7-8 μm to a large oocyst of 35-40 μm) in the mosquitoes. A few recent RNA-Seq analyses of P. vivax infected mosquitoes have been valuable to understand the ookinete and oocyst stage of P. vivax which reveals the alteration of several transcripts in the gut after 18 hours and 7 days post-infection in mosquito Anopheles dirus [39]. Notably, the authors identified several genes such as Anoctamin 6 (ANO6; ADIR005670) and Fibroblast Growth Factor (FGF; ADIR008464), which may likely have immune regulation of P. vivax growth in the gut of the mosquito.…”

Section: Post-invasion Strategy Of P Vivax (Development Of Oocyst)mentioning

confidence: 99%

Molecular Dynamics of Mosquito-Plasmodium vivaxInteraction: A Smart Strategy of Parasitism

Chauhan¹,

Tevatiya²,

Kumari³

et al. 2021

Cell Interaction - Molecular and Immunological Basis for Disease Management

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Parallel to Plasmodium falciparum, P. vivax is a fast emerging challenge to control malaria in South-East Asia regions. Owing to unique biological differences such as the preference for invading reticulocytes, early maturation of sexual stages during the infection, the formation of hypnozoites, unavailability of in-vitro culture, the molecular relation of P. vivax development inside the mosquito host is poorly known. In this chapter, we briefly provide a basic overview of Mosquito-Plasmodium interaction and update current knowledge of tissue-specific viz. midgut, hemocyte, and salivary glands- molecular dynamics of Plasmodium vivax interaction during its developmental transformation inside the mosquito host, in specific.

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Transcriptome analysis of Anopheles dirus and Plasmodium vivax at ookinete and oocyst stages

Cited by 11 publications

References 46 publications

Differential transcriptomic response ofAnopheles arabiensistoPlasmodium vivaxandPlasmodium falciparuminfection

Differential transcriptomic response ofAnopheles arabiensistoPlasmodium vivaxandPlasmodium falciparuminfection

Anopheles dirus yellow‐g mediates Plasmodium vivax infection

Molecular Dynamics of Mosquito-Plasmodium vivaxInteraction: A Smart Strategy of Parasitism

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