Transcriptome analysis of Corynebacterium pseudotuberculosis-infected spleen of dairy goats

Fu, Mingzhe; Su, Hong; Su, Zhanqiang; Yin, Zheng; Jin, Jian; Wang, Lixiang; Zhang, Qi; Xu, Xingang

doi:10.1016/j.micpath.2020.104370

Cited by 8 publications

(8 citation statements)

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“…The expression of MHC genes is altered during mycobacterial infection in cattle [ 42 , 53 , 54 ]. In transcriptome analysis of the Cp -infected spleen of dairy goats, the MHC class I protein complex was identified as one of the most enriched terms [ 21 ]. The genes for CD1A and CD1E molecules structurally related to MHC, which present lipids of microbial origin to T-cell receptors, were differentially expressed only in the transcriptome of the diseased ewes.…”

Section: Discussionmentioning

confidence: 99%

“…The formation of pyogranulomas, which are characteristic of C. pseudotuberculosis infection, involves both humoral and cell-mediated immunity, a complex process that requires the activation of macrophages and T lymphocytes [ 18 , 19 , 20 ]. Recent advances in high-throughput genomic technologies have enabled the first comprehensive profiling of the goat spleen transcriptome and proteome in response to experimental infection with Cp [ 21 , 22 , 23 ]. Currently, genome-wise approaches and the generation of big data are considered promising selection-associated techniques that can provide animal farming industries with the ability to cope with the issues caused by diseases through breeding for health traits [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Kyselová

Tichý

Sztankóová

et al. 2023

Animals

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Caseous lymphadenitis (CL) is a chronic contagious disease that affects small ruminants and is characterized by the formation of pyogranulomas in lymph nodes and other organs. However, the pathogenesis of this disease and the response of the host genome to infection are not yet fully understood. This study aimed to investigate the whole blood transcriptome and evaluate differential gene expression during the later stages of CL in naturally infected ewes. The study included diseased, serologically positive (EP), exposed, serologically negative (EN) ewes from the same infected flock and healthy ewes (CN) from a different flock. RNA sequencing was performed using the Illumina NextSeq system, and differential gene expression was estimated using DESeq2 and Edge R approaches. The analysis identified 191 annotated differentially expressed genes (DEGs) in the EP group (102 upregulated and 89 downregulated) and 256 DEGs in the EN group (106 upregulated and 150 downregulated) compared to the CN group. Numerous immunoregulatory interactions between lymphoid and nonlymphoid cells were influenced in both EP and EN ewes. Immune DEGs were preferentially assigned to antigen presentation through the MHC complex, T lymphocyte-mediated immunity, and extracellular matrix interactions. Furthermore, the EP group showed altered regulation of cytokine and chemokine signaling and activation and recombination of B-cell receptors. Conversely, NF-kappa B signaling, apoptosis, and stress response were the main processes influenced in the EN group. In addition, statistically significant enrichment of the essential immune pathways of binding and uptake of ligands by scavenger receptors in EP and p53 signaling in the EN group was found. In conclusion, this study provides new insights into the disease course and host–pathogen interaction in naturally CL-infected sheep by investigating the blood transcriptome.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Kyselová

Tichý

Sztankóová

et al. 2023

Animals

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“…This analysis was performed as previously described [ 13 ]. Briefly, three spleen samples from each group were included for RNA sequencing.…”

Section: Methodsmentioning

confidence: 99%

Combined Transcriptomic and Proteomic of Corynebacterium pseudotuberculosis Infection in the Spleen of Dairy Goats

Cheng

et al. 2022

Animals

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Corynebacterium pseudotuberculosis (C. pseudotuberculosis) is a zoonotic chronic infectious disease. It mainly occurs in dairy goats reared in herds, and once it invades the dairy goats, it is difficult to completely remove it, causing great harm to the development of the sheep industry. This study mainly was based on TMT-based quantitative proteomics and RNA-seq methods to measure the spleen samples of infected dairy goats at different time periods. Nine four-month-old dairy goats were divided into three groups, with three goats in each group. The dairy goats in the first group (NC group) were inoculated with 1.0 mL of sterilized normal saline subcutaneously, and the second (72 h group) and third groups (144 h group) were inoculated with 1.0 mL of 1 × 107 cfu/mL bacterial solution subcutaneously in the neck. Significant changes in the protein and mRNA expression were observed in different infection and control groups. In the 72 h group, 85 genes with differential genes and proteins were up-regulated and 91 genes were down-regulated in this study. In the 144 h group, 38 genes with differential genes and proteins were up-regulated and 51 genes were down-regulated. It was found that 21 differentially expressed genes and proteins were co-up-regulated in the two groups. There were 20 differentially expressed genes and proteins which were co-down-regulated in both groups. The 72 h group were mainly enriched in protein processing in the endoplasmic reticulum, lysosome, amino sugar and nucleotide sugar metabolism and the estrogen signaling pathway. In the 144 h group, they were protein processing in the endoplasmic reticulum pathway which was enriched by mRNA–proteins pairs co-upregulated by the five pairs. The combined transcriptomic and proteomic analyses were performed to provide insights into the effects of C. pseudotuberculosis through several regulatory features and pathways. We found that in the early stage of infection (72 h), the co-upregulated gene–protein pairs were enriched in multiple pathways, which jointly defended against a bacterial invasion. However, in the later stages of infection (144 h), when the disease stabilizes, a few co-upregulated gene–protein pairs played a role in protein processing in the endoplasmic reticulum pathway. In addition, the mRNA and protein expressions of dairy goats infected with the bacteria at different periods of time indicated the adaptability of dairy goats to the bacteria. At the same time, it guides us to carry out a corresponding treatment and feeding management for dairy goats according to different periods of time.

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“…A recent study used RNA-Seq to analyze C. pseudotuberculosis-infected spleens of dairy goats and found many iron-related genes differentially expressed in order to reduce iron availability. The authors hypothesized that C. pseudotuberculosis evolved an iron acquisition mechanism to manage this reduction [98]. The expression of DtxR, the master regulator of iron, is downregulated under iron limitation, directly and indirectly influencing the expression of several genes in C. pseudotuberculosis [43].…”

Section: Metalloregulation: Iron Uptakementioning

confidence: 99%

The Transcriptional Regulatory Network of Corynebacterium pseudotuberculosis

Parise

Gomide

et al. 2021

Microorganisms

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Corynebacterium pseudotuberculosis is a Gram-positive, facultative intracellular, pathogenic bacterium that infects several different hosts, yielding serious economic losses in livestock farming. It causes several diseases including oedematous skin disease (OSD) in buffaloes, ulcerative lymphangitis (UL) in horses, and caseous lymphadenitis (CLA) in sheep, goats and humans. Despite its economic and medical-veterinary importance, our understanding concerning this organism’s transcriptional regulatory mechanisms is still limited. Here, we review the state of the art knowledge on transcriptional regulatory mechanisms of this pathogenic species, covering regulatory interactions mediated by two-component systems, transcription factors and sigma factors. Key transcriptional regulatory players involved in virulence and pathogenicity of C. pseudotuberculosis, such as the PhoPR system and DtxR, are in the focus of this review, as these regulators are promising targets for future vaccine design and drug development. We conclude that more experimental studies are needed to further understand the regulatory repertoire of this important zoonotic pathogen, and that regulators are promising targets for future vaccine design and drug development.

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Transcriptome analysis of Corynebacterium pseudotuberculosis-infected spleen of dairy goats

Cited by 8 publications

References 37 publications

Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Combined Transcriptomic and Proteomic of Corynebacterium pseudotuberculosis Infection in the Spleen of Dairy Goats

The Transcriptional Regulatory Network of Corynebacterium pseudotuberculosis

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