Transcriptome analysis of gene expression in Chlorella vulgaris under salt stress

Abdellaoui, Najib; Kim, Min Jeong; Choi, Tae-Jin

doi:10.1007/s11274-019-2718-6

Cited by 20 publications

(15 citation statements)

References 51 publications

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“…This has already been showed for C. vulgaris cells submitted to the same saline stress (0.1 M) as in this study [28]. In addition, a genomic study has showed that under saline stress conditions, the expression of a large number of genes was downregulated, in particular genes involved in the photosystem light-harvesting pathways as well as genes involved in protein synthesis and stability [47].…”

Section: Figure 5 Probing the Hydrophobic Properties Of C Vulgaris Ce...supporting

confidence: 81%

Combining AFM, XPS and chemical hydrolysis to understand the complexity and dynamics of C. vulgaris cell wall composition and architecture

et al. 2023

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Section: Figure 5 Probing the Hydrophobic Properties Of C Vulgaris Ce...supporting

confidence: 81%

Combining AFM, XPS and chemical hydrolysis to understand the complexity and dynamics of C. vulgaris cell wall composition and architecture

et al. 2023

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“…The salt experiment and transcriptome analysis were conducted as in the work of Abdellaoui et al . [ 22 ]. Briefly, C. vulgaris PKVL7422 (KCTC13361BP) was cultured in mBG11 medium [ 23 ] at 20°C with a light source of 52 μmol photons/m 2 /s 1 .…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, C. vulgaris PKVL7422 (KCTC13361BP) was cultured in mBG11 medium [ 23 ] at 20°C with a light source of 52 μmol photons/m 2 /s 1 . Cultures were treated with 170 mM (1%) or 510 mM (3%) NaCl for 2 h and 4 h have described previous [ 22 ]. RNA was extracted from the concentrated cells and subjected to transcriptome analysis [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

“…Cultures were treated with 170 mM (1%) or 510 mM (3%) NaCl for 2 h and 4 h have described previous [ 22 ]. RNA was extracted from the concentrated cells and subjected to transcriptome analysis [ 22 ]. The expression levels of transcripts in treated groups were compared with the control group via RNA-Seq by Expectation-Maximization v1.2.15 based on Fragments Per Kilobase of transcript per Million (FPKM) values.…”

Section: Methodsmentioning

confidence: 99%

“…Salt stress can increase Chlorella biomass [ 18 ] and improve gene expression in plants (BY-2 cells and rice), bacteria ( Halomonas elongate ), and microalgae ( Chlamydomonas reinhardtii ) [ 19 - 21 ]. In a previous study, we investigated the effects of a high-salt environment on Chlorella and identified genes that were upregulated because of salt stress [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of a Salt Inducible Promoter from Chlorella vulgaris PKVL7422

Kim

Abdellaoui

et al. 2023

J. Microbiol. Biotechnol.

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Chlorella is a eukaryotic organism that can be used as an industrial host to produce recombinant proteins. In this study, a salt-inducible promoter (SIP) was isolated from the freshwater species Chlorella vulgaris PKVL7422 from the screening of genes that were upregulated after salt treatment. Several cis -acting elements, including stress response elements, were identified in the isolated SIP. Moreover, the Gaussia luciferase gene was cloned after the SIP and transformed into C. vulgaris to test the inducibility of this promoter. Reexamination of transcriptome of C. vulgaris revealed that genes involved in the synthesis of methyl jasmonic acid (MeJA), gibberellin (GA), and abscisic acid (ABA) were upregulated when C. vulgaris was treated with salt. Furthermore, the expression level of recombinant luciferase increased when the transformed C. vulgaris was treated with salt and MeJA, GA, and ABA. This study represents the first report of the C. vulgaris SIP and highlights how transformed microalgae could be used for robust expression of recombinant proteins.

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Enhancement of heat tolerance by salt stress in Tetraselmis striata CTP4: impacts on HSP gene expression, pigments, and proximal composition

Santos,

Pereira,

Schüler

et al. 2024

J Appl Phycol

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As the world average temperature is on the rise and heat waves are becoming more prevalent, microalgal producers have been facing significant challenges regarding the time periods during which they are able to grow less thermotolerant microalgae in outdoor production facilities. Therefore, it is urgent to explore how microalgae cope with thermal stress and under which growth conditions tolerance to temperatures close to or higher than their maximum thermal threshold is induced. For this purpose, Tetraselmis striata CTP4, a euryhaline microalga known for its thermotolerance, was selected. Tetraselmis striata belongs to the Chlorodendrophyceae, a clade that branched off early from other "core chlorophyte" clades, usually comprised of microalgae able to colonise freshwater habitats. Here, we present compelling evidence that the ability of this microalga to withstand otherwise lethal thermal upshifts to 40 °C is induced by exposure to higher salinity (35 ppt). In contrast, this response is abrogated at lower salinities. Concomitantly, the expression of genes encoding HSP70 and HSP100, two heat shock proteins known to mediate thermotolerance and tolerance to other stresses (e.g., salt stress) in fungi, animals and plants, was enhanced when exposed to both heat stress and higher salinities. This suggests that cross-protective mechanisms against abiotic stress appeared early during the evolution of the core chlorophytes and of Archaeplastida in general. This knowledge can be used to select novel strains and growth conditions that promote thermotolerance in microalgae that are grown in outdoor industrial production facilities in environments where heat waves are expected.

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Transcriptome analysis of gene expression in Chlorella vulgaris under salt stress

Cited by 20 publications

References 51 publications

Combining AFM, XPS and chemical hydrolysis to understand the complexity and dynamics of C. vulgaris cell wall composition and architecture

Combining AFM, XPS and chemical hydrolysis to understand the complexity and dynamics of C. vulgaris cell wall composition and architecture

Isolation and Characterization of a Salt Inducible Promoter from Chlorella vulgaris PKVL7422

Enhancement of heat tolerance by salt stress in Tetraselmis striata CTP4: impacts on HSP gene expression, pigments, and proximal composition

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