Transcriptome analysis of Neocaridina denticulate sinensis under copper exposure

Xing, Kefan; Liu, Yujie; Yan, Congcong; Zhou, Yongzhao; Sun, Yuying; Su, Naike; Fang, Yang; Xie, Shouqi; Zhang, Jiquan

doi:10.1016/j.gene.2020.145098

Cited by 24 publications

(11 citation statements)

References 48 publications

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“…In addition, the alteration of endocytosis during stress may counteract deleterious effects through switching on or amplifying specific cellular pathways ( Lopez-Hernandez et al, 2020 ). Previous studies also suggested that the significant change of endocytosis pathway could be induced by adverse environmental conditions in aquatic animals, such as nitrite ( Yu et al, 2019 ), salinity ( Qin et al, 2020 ), copper exposure ( Xing et al, 2021 ) and cadmium exposure ( Zhang et al, 2021 ). Vega et al (2010) suggested the upregulated endocytosis played an important role in the recovery from damage under stress conditions.…”

Section: Discussionmentioning

confidence: 99%

Effects of stocking density on the growth performance, mitophagy, endocytosis and metabolism of Cherax quadricarinatus in integrated rice–crayfish farming systems

et al. 2022

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Red claw crayfish (Cherax quadricarinatus) is an economic freshwater shrimp with great commercial potential. However, the suitable stocking density of C. quadricarinatus is still unclear in integrated rice–crayfish farming system. Thus, this study aimed to investigate the effects of stocking density on growth performance, mitophagy, endocytosis and metabolism of C. quadricarinatus. The C. quadricarinatus was reared at low density (LD, 35.73 g/m2), middle density (MD, 71.46 g/m2) and high density (HD, 107.19 g/m2) in an integrated rice–crayfish farming system. After 90 days of farming, the growth performance of C. quadricarinatus significantly decreased in the MD and HD groups relative to that in the LD group. The HD treatment caused oxidative stress and lipid peroxidation at the end of the experiment in hepatopancreas. Transcriptome analysis showed that there were 1,531 DEGs (differently expressed genes) between the LD group and HD group, including 1,028 upregulated genes and 503 downregulated genes. KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis indicated that the DEGs were significantly enriched in endocytosis and mitophagy pathways. Meanwhile, four lipid metabolism pathways, including biosynthesis of unsaturated fatty acids, fatty acid biosynthesis, glycerolipid metabolism and glycerophospholipid metabolism, exhibited an upregulated tendency in the HD group. In conclusion, our data showed that when the stocking density reached up to 207.15 g/m2 in HD group, the growth performance of C. quadricarinatus was significantly inhibited in this system. Meanwhile, the data indicated that C. quadricarinatus may respond to the stressful condition via activating antioxidant defense system, endocytosis, mitophagy and metabolism-related pathways in hepatopancreas.

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Section: Discussionmentioning

confidence: 99%

Effects of stocking density on the growth performance, mitophagy, endocytosis and metabolism of Cherax quadricarinatus in integrated rice–crayfish farming systems

et al. 2022

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“…As we known, invertebrates do not possess speci c immunity [37], so they have to depend on innate immune system to prevent invaders [22]. When aquatic organisms are exposed to stressful environment, their bodies generate amounting ROS against it.…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, we suspected that N. denticulata sinensis act as biomarker to detect the change of water environment. It is said that hepatopancreas of crustaceans are places of immune molecules synthesis, and, at same time, hepatopancreas of crustaceans also are main tissues to store or degrade metal ions [22,46]. Herein, the result of qRT-PCR of different times of hepatopancreas under exposure of Cu 2+ indicated that expression of Nd-ecCu/Zn-SOD mRNA was up-regulated signi cantly to eliminate ROS during short period and after that it was rapidly decreased, which indicated that Nd-ecCu/Zn-SOD might play an important role in immune response.…”

Section: Discussionmentioning

confidence: 99%

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An extracellular Cu/Zn superoxide dismutase from Neocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein

Feng¹,

Gao²,

Kong³

et al. 2022

Fish & Shellfish Immunology

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“…This makes them readily available and accessible to culture in the laboratory. Additionally, the draft genome [48] and transcriptome [49][50][51][52] of N. denticulata have been decoded, making them an ideal model for functional genomic studies [53]. This study investigated N. denticulata as an ideal aquatic nontarget invertebrate model for pesticide toxicity analysis.…”

Section: Discussionmentioning

confidence: 99%

Exploiting the Freshwater Shrimp Neocaridina denticulata as Aquatic Invertebrate Model to Evaluate Nontargeted Pesticide Induced Toxicity by Investigating Physiologic and Biochemical Parameters

et al. 2021

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As a nicotinoid neurotoxic insecticide, imidacloprid (IMI) works by disrupting nerve transmission via nicotinic acetylcholine receptor (nAChR). Although IMI is specifically targeting insects, nontarget animals such as the freshwater shrimp, Neocaridina denticulata, could also be affected, thus causing adverse effects on the aquatic environment. To investigate IMI toxicity on nontarget organisms like N. denticulata, their physiology (locomotor activity, heartbeat, and gill ventilation) and biochemical factors (oxidative stress, energy metabolism) after IMI exposure were examined. IMI exposure at various concentrations (0.03125, 0.0625, 0.125, 0.25, 0.5, and 1 ppm) to shrimp after 24, 48, 72 h led to dramatic reduction of locomotor activity even at low concentrations. Meanwhile, IMI exposure after 92 h caused reduced heartbeat and gill ventilation at high concentrations. Biochemical assays were performed to investigate oxidative stress and energy metabolism. Interestingly, locomotion immobilization and cardiac activity were rescued after acetylcholine administration. Through molecular docking, IMI demonstrated high binding affinity to nAChR. Thus, locomotor activity and heartbeat in shrimp after IMI exposure may be caused by nAChR blockade and not alterations caused by oxidative stress and energy metabolism. To summarize, N. denticulata serves as an excellent and sensitive aquatic invertebrate model to conduct pesticide toxicity assays that encompass physiologic and biochemical examinations.

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Transcriptome analysis of Neocaridina denticulate sinensis under copper exposure

Cited by 24 publications

References 48 publications

Effects of stocking density on the growth performance, mitophagy, endocytosis and metabolism of Cherax quadricarinatus in integrated rice–crayfish farming systems

Effects of stocking density on the growth performance, mitophagy, endocytosis and metabolism of Cherax quadricarinatus in integrated rice–crayfish farming systems

An extracellular Cu/Zn superoxide dismutase from Neocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein

Exploiting the Freshwater Shrimp Neocaridina denticulata as Aquatic Invertebrate Model to Evaluate Nontargeted Pesticide Induced Toxicity by Investigating Physiologic and Biochemical Parameters

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