Transcriptome analysis of neutrophils after endurance exercise reveals novel signaling mechanisms in the immune response to physiological stress

Neubauer, Oliver; Sabapathy, Surendran; Lazarus, Ross; Jowett, Jeremy B. M.; Desbrow, Ben; Peake, Jonathan M.; Cameron‐Smith, David; Haseler, Luke J.; Wagner, Karl‐Heinz; Bulmer, Andrew Cameron

doi:10.1152/japplphysiol.00143.2013

Cited by 63 publications

(80 citation statements)

References 57 publications

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“…HSPA1A. Similar results were obtained by Neubauer et al [14]) who examined gene expression 2h after exercise consisting of 1h running and cycling. Furthermore, some authors reported lower levels of HSPA1A expression in trained subjects in comparison to untrained group, as measured after physical activity [14,15].…”

Section: Introductionsupporting

confidence: 85%

“…Radom-Azik et al [13] and Neubauer et al [14] stated that the upregulation of genes related to apoptosis and immune response in neutrophils, represents an integrated response to various physiological dysfunctions. The presented results, obtained from the experiment which was carried out under heat-stress conditions, are consistent with those reported by Neubauer [14] and Buttner [15], who noted lower expression of genes encoding HSPs and interleukins in trained vs. untrained people after exercise using the same load.…”

Section: Discussionmentioning

confidence: 99%

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Effects of sauna bathing on stress-related genes expression in athletes and non-athletes

Żychowska

Półrola

Chruściński

et al. 2017

Ann Agric Environ Med.

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Introduction and objective. Heat stress induces the expression of genes encoding heat-shock proteins and immune response mediators. The aim of this study was to determine the differences in the expression of genes encoding heat-shock proteins 70 kDa and27 kDa, interleukin 6, interleukin 10and C-reactive protein, between athletes and non-athletes after sauna bathing. Materials and method. Athletes (n=9) and non-athletes (n=9) were exposed to a Finnish sauna twice during one session at a temperature of 98.2 °C and humidity of 10% ± 2%, with a 5 min break for cooling down under a shower. The groups did not differ in terms of age, height or body mass. Blood samples were taken before and after sauna exposure in order to assess gene expression, using reverse transcription polymerase chain reaction. Results. Differences were observed in leukocyte mRNA levels of tested genes between athletes and non-athletes. In the non-athlete group, all the tested genes were expressed at higher levels as a response to the same heat challenge. Conclusion. It appears that expression of stress-related genes induced by heat stress is dependent on the level of physical activity.

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Section: Introductionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Effects of sauna bathing on stress-related genes expression in athletes and non-athletes

Żychowska

Półrola

Chruściński

et al. 2017

Ann Agric Environ Med.

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“…Apart from the Hsp, additional sterile inflammation signals released into the circulation by stressed or/and injured cells not assessed in the current investigation may also influence TLR signaling pathways in PBMCs. In this line, Neubauer et al (2013) suggest that DAMPs, hypothetically originating from damaged skeletal muscle tissue, are associated with the activation of TLRs in neutrophils. Moreover, it is important to highlight that the function of these endogenous "danger signals" might be also altered with aging, as a result of increased oxidative stress and inflammation in old subjects (Murlasits et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…However, there are other structures, called damage-associated molecular patterns (DAMPs), that are endogenous ligands released from damaged or stressed host tissue which also modulate the activation of both TLRs (Asea et al 2002). Under normal physiological conditions, these factors are hidden, but after a stress stimulus, they are released into the extracellular environment to be recognized by the host immune system (Neubauer et al 2013). Numerous studies have showed that apoptotic and necrotic cells release DAMP molecules such as high-mobility group box-1 (HMGB1), S-100 proteins, heat shock proteins, hyaluronan, surfactant protein, interferon-alpha, uric acid, fibronectin, beta defensin, and cardiolipin, which trigger a "sterile inflammatory response" following tissue damage (Martin-Murphy et al 2010;Neubauer et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Under normal physiological conditions, these factors are hidden, but after a stress stimulus, they are released into the extracellular environment to be recognized by the host immune system (Neubauer et al 2013). Numerous studies have showed that apoptotic and necrotic cells release DAMP molecules such as high-mobility group box-1 (HMGB1), S-100 proteins, heat shock proteins, hyaluronan, surfactant protein, interferon-alpha, uric acid, fibronectin, beta defensin, and cardiolipin, which trigger a "sterile inflammatory response" following tissue damage (Martin-Murphy et al 2010;Neubauer et al 2013). Among DAMPs, heat shock protein 70 kDa (Hsp70) has an important role in the activation of both TLR2 and TLR4 (Asea et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Role of Toll-like receptor 2 and 4 signaling pathways on the inflammatory response to resistance training in elderly subjects

Rodriguez‐Miguelez

Fernandez‐Gonzalo

Almar

et al. 2014

AGE

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This study assessed the effects of a resistance exercise training program on the inflammatory response associated with Toll-like receptor (TLR) 2 and TLR4 signaling pathways in senior participants. Twenty-six healthy subjects (age, 69.5±1.3) were randomized to a training (TG; n=16) or a control (CG; n=10) group. TG performed an 8-week resistance training program, while CG followed their daily routines. Peripheral blood mononuclear cells were isolated from blood samples obtained before and after the intervention, and levels of proteins involved in the TLR2, TLR4, and myeloid differentiation primary response gene 88 (MyD88)-dependent and MyD88-independent pathways were analyzed. The inflammatory status was evaluated through messenger RNA (mRNA) and protein content of interleukin (IL)-10 and tumor necrosis factor alpha (TNF-α) and plasma levels of C-reactive protein (CRP). After the 8-week resistance training, TLR2 and TLR4 protein expression was reduced in TG. MyD88, p65, phosphop38, TIR domain-containing adaptor inducing interferon (TRIF), IKKi/IKKε, phospho-interferon regulatory factor (IRF) 3, and phosho-IRF7 were also downregulated in TG after the intervention. The training program induced an increase of phospho-extracellular signal-regulated kinases 1 and 2 (ERK1/2) and Hsp70 and a reduction of Hsp60. While TNF-α mRNA and protein values remained unchanged in both TG and CG, IL-10 mRNA and protein content were upregulated in TG after the intervention. CRP values decreased in TG only. The increase in Hsp70 negatively correlated with TLR2 and TLR4 downregulation. These data suggest that resistance exercise may represent an effective tool to ameliorate the pro-inflammatory status of old participants through an attenuation of MyD88-dependent and MyD88-independent TLR2 and TLR4 signaling pathways.

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Activity‐Dependent Induction of Younger Biological Phenotypes

Lissek

2022

Advanced Biology

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In several mammalian species, including humans, complex stimulation patterns such as cognitive and physical exercise lead to improvements in organ function, organism health and performance, as well as possibly longer lifespans. A framework is introduced here in which activity‐dependent transcriptional programs, induced by these environmental stimuli, move somatic cells such as neurons and muscle cells toward a state that resembles younger cells to allow remodeling and adaptation of the organism. This cellular adaptation program targets several process classes that are heavily implicated in aging, such as mitochondrial metabolism, cell–cell communication, and epigenetic information processing, and leads to functional improvements in these areas. The activity‐dependent gene program (ADGP) can be seen as a natural, endogenous cellular reprogramming mechanism that provides deep insight into the principles of inducible improvements in cell and organism function and can guide the development of therapeutic approaches for longevity. Here, these ADGPs are analyzed, exemplary critical molecular nexus points such as cAMP response element‐binding protein, myocyte enhancer factor 2, serum response factor, and c‐Fos are identified, and it is explored how one may leverage them to prevent, attenuate, and reverse human aging‐related decline of body function.

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Transcriptome analysis of neutrophils after endurance exercise reveals novel signaling mechanisms in the immune response to physiological stress

Abstract: AC. Transcriptome analysis of neutrophils after endurance exercise reveals novel signaling mechanisms in the immune response to physiological stress.

Cited by 63 publications

References 57 publications

Effects of sauna bathing on stress-related genes expression in athletes and non-athletes

Effects of sauna bathing on stress-related genes expression in athletes and non-athletes

Role of Toll-like receptor 2 and 4 signaling pathways on the inflammatory response to resistance training in elderly subjects

Activity‐Dependent Induction of Younger Biological Phenotypes

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