Transcriptome analysis reveals molecular mechanisms of sclerotial development in the rice sheath blight pathogen Rhizoctonia solani AG1-IA

Shu, Canwei; Zhao, Mei; Anderson, Jonathan P.; Garg, Gagan; Singh, Karam B.; Zheng, Wenbo; Wang, Chenjiaozi; Yang, Mei; Zhou, Erxun

doi:10.1007/s10142-019-00677-0

Cited by 26 publications

(32 citation statements)

References 55 publications

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“…Rice, one of the major food crops, is continuously threatened by various pathogenic microbes. Pressingly, R. solani causes rice sheath blight, one of the most severe fungi diseases in rice, and poses a significant threat to grain yield (Shu et al, 2019). Breeding for disease-resistant varieties is considered to be the most effective and eco-friendly method for disease control.…”

Section: Discussionmentioning

confidence: 99%

The Effector AGLIP1 in Rhizoctonia solani AG1 IA Triggers Cell Death in Plants and Promotes Disease Development Through Inhibiting PAMP-Triggered Immunity in Arabidopsis thaliana

Peng

Wang

et al. 2019

Front. Microbiol.

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Rhizoctonia solani, one of the most detrimental necrotrophic pathogens, causes rice sheath blight and poses a severe threat to production. Focus on the function of effectors secreted by necrotrophic pathogens during infection has grown rapidly in recent years. However, little is known about the virulence and mechanisms of these proteins. In this study, we performed functional studies on putative effectors in R. solani and revealed that AGLIP1 out of 13 putative effectors induced cell death in Nicotiana benthamiana. AGLIP1 was also demonstrated to trigger cell death in rice protoplasts. The predicted lipase active sites and signal peptide (SP) of this protein were required for the cell death-inducing ability. AGLIP1 was greatly induced during R. solani infection in rice sheath. The AGLIP1’s virulence function was further demonstrated by transgenic technology. The pathogenesis-related genes induced by pathogen-associated molecular pattern and bacteria were remarkably inhibited in AGLIP1-expressing transgenic Arabidopsis lines. Ectopic expression of AGLIP1 strongly facilitated disease progression in Arabidopsis caused by the type III secretion system-defective mutant from Pseudomonas syringae pv. tomato DC3000. Collectively, these results indicate that AGLIP1 is a possible effector that plays a significant role in pathogen virulence through inhibiting basal defenses and promoting disease development in plants.

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Section: Discussionmentioning

confidence: 99%

The Effector AGLIP1 in Rhizoctonia solani AG1 IA Triggers Cell Death in Plants and Promotes Disease Development Through Inhibiting PAMP-Triggered Immunity in Arabidopsis thaliana

Peng

Wang

et al. 2019

Front. Microbiol.

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“…It is very difficult to find asexual spores of R. solani and this fungus survives in an unfavorable environment through sclerotia formed by tightly interweaving mycelia (Willetts and Bullock 1992 ; Sun et al 2020b ). Sclerotia are developed on infected plants late in the cropping season and are dropped into soil or water (Shu et al 2019 ). Dormant sclerotia can live in the soil for several years and serve as the primary inocula for the next cropping season (Singh et al 2019 ).…”

Section: Overview Of the Pathogens And Diseasesmentioning

confidence: 99%

“…In China, RSB was first reported in 1934, and it has become the second most important disease in rice at present, causing a yield loss of 10 ~ 30 % every year, even up to 50 % in the rice-growing region of Yangtze river valley and South China in epidemic years (Yu et al 2019 ; Zhu et al 2019 ). The annual disease area in China is about 15 ~ 20 million hm 2 (Bernardes-de-Assis et al 2009 ; Shu et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Strategies to Manage Rice Sheath Blight: Lessons from Interactions between Rice and Rhizoctonia solani

Wei

et al. 2021

Rice

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Rhizoctonia solani is an important phytopathogenic fungus with a wide host range and worldwide distribution. The anastomosis group AG1 IA of R. solani has been identified as the predominant causal agent of rice sheath blight, one of the most devastating diseases of crop plants. As a necrotrophic pathogen, R. solani exhibits many characteristics different from biotrophic and hemi-biotrophic pathogens during co-evolutionary interaction with host plants. Various types of secondary metabolites, carbohydrate-active enzymes, secreted proteins and effectors have been revealed to be essential pathogenicity factors in R. solani. Meanwhile, reactive oxygen species, phytohormone signaling, transcription factors and many other defense-associated genes have been identified to contribute to sheath blight resistance in rice. Here, we summarize the recent advances in studies on molecular interactions between rice and R. solani. Based on knowledge of rice-R. solani interactions and sheath blight resistance QTLs, multiple effective strategies have been developed to generate rice cultivars with enhanced sheath blight resistance.

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“…effector proteins and carbohydrate-active enzymes (CAZymes) (13). Additionally, analysis of differentially expressed genes in different isolates has enabled researchers to predict the adaptive behavior of this fungus in different hosts and the associated virulence (14,15). However, the majority of this information has come from the analysis of isolates belonging to only a small number of AGs for which complete genome and/or transcriptome sequences are available.…”

Section: Introductionmentioning

confidence: 99%

Pangenome analysis of the soil-borne fungal phytopathogenRhizoctonia solaniand development of a comprehensive web resource: RsolaniDB

Kaushik

Roberts²,

Ramaprasad

et al. 2020

Preprint

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Rhizoctonia solani is a collective group of genetically and pathologically diverse basidiomycetous fungus that damages economically important crops. Its isolates are classified into 13 Anastomosis Groups (AGs) and subgroups having distinctive morphology and host range. The genetic factors driving the unique features of R. solani pathology are not well characterized due to the limited availability of its annotated genomes. Therefore, we performed genome sequencing, assembly, annotation and functional analysis of 12 R. solani isolates covering 7 AGs and selected subgroups (AG1-IA, AG1-IB, AG1-IC, AG2-2IIIB, AG3-PT (isolates Rhs 1AP and the hypovirulent Rhs1A1), AG3-TB, AG4-HG-I (isolates Rs23 and R118-11), AG5, AG6, and AG8), in which six genomes are reported for the first time, wherein we discovered unique and shared secretomes, CAZymes, and effectors across the AGs. Using a pangenome comparative analysis of 12 R. solani isolates and 15 other basidiomycetes, we also elucidated the molecular factors potentially involved in determining the AG-specific host preference, and the attributes distinguishing them from other Basidiomycetes. Finally, we present the largest repertoire of R. solani genomes and their annotated components as a comprehensive database, viz. RsolaniDB, with tools for large-scale data mining, functional enrichment and sequence analysis not available with other state-of-the-art platforms, to assist mycologists in formulating new hypotheses.

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Transcriptome analysis reveals molecular mechanisms of sclerotial development in the rice sheath blight pathogen Rhizoctonia solani AG1-IA

Cited by 26 publications

References 55 publications

The Effector AGLIP1 in Rhizoctonia solani AG1 IA Triggers Cell Death in Plants and Promotes Disease Development Through Inhibiting PAMP-Triggered Immunity in Arabidopsis thaliana

The Effector AGLIP1 in Rhizoctonia solani AG1 IA Triggers Cell Death in Plants and Promotes Disease Development Through Inhibiting PAMP-Triggered Immunity in Arabidopsis thaliana

Strategies to Manage Rice Sheath Blight: Lessons from Interactions between Rice and Rhizoctonia solani

Pangenome analysis of the soil-borne fungal phytopathogenRhizoctonia solaniand development of a comprehensive web resource: RsolaniDB

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