Transcriptome Analysis Reveals the Mechanism Underlying the Production of a High Quantity of Chlorogenic Acid in Young Leaves of Lonicera macranthoides Hand.-Mazz

Chen, Zexiong; You, Yuming; Lan, Jianbin; Liu, Yiqing; Li, Zhengguo

doi:10.1371/journal.pone.0137212

“…In addition, the following downstream two enzyme genes, 4CL and Cinnamate 4-hydroxylase (C4H), which catalyzed the formation of p-Coumaroy-CoA, did not express signi cantly differently among comparisons in this research. These results were quite different from other higher plants, for them PAL, 4CL and C4H were key enzymes in CGA accumulation [44]. However, the intriguing phenomenon occurred on three common DEGs HCT, CCR and CCoMAT in the phenylalanine pathway.…”

Section: Discussioncontrasting

confidence: 76%

Integrated transcriptome, small RNA and degradome sequencing approaches proffer insights into chlorogenic acid (CGA) biosynthesis in leafy sweet potato

Liu

¹

,

Su

²

,

Wang

³

et al. 2020

Preprint

0

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Background: Phenolic compounds play key roles in health protection and leafy sweet potato is an excellent source of total phenolics (TP). The chlorogenic acid (CGA) family, which includes caffeoylquinic acid (CQA), 3,4-O-dicaffeoylquinic acid (3,4-diCQA), 3,5-O-dicaffeoylquinic acid (3,5-diCQA) and 4,5-O-dicaffeoylquinic acid (4,5-diCQA) , constitutes the major components of phenolic compounds in leafy sweet potato. However, the mechanism of CGA biosynthesis in leafy sweet potato is unclear. The objective of present study is to dissect the mechanisms of CGA biosynthesis by using transcriptome, small RNA (sRNA) and degradome sequencing. Results: Transcriptome sequencing of twelve samples (triple replicates) from one low-CGA content genotype and one high-CGA content genotype at two stages (65 and 85 days after planting) identified a total of 2333 common differentially expressed genes (DEGs). The enriched DEGs were related to photosynthesis, starch and sucrose metabolism and phenylpropanoid biosynthesis. In this study, functional genes CCR , CCoAOMT and HCT in the CGA biosynthetic pathway were uniformly downregulated, indicating the way to lignin was altered, and two possible CGA biosynthetic routes were hypothesized. The sRNA sequencing identified a total of 38 DE miRNAs. Using in silico approaches, 1799 targets were predicated for 38 DE miRNAs. The target genes were enriched in lignin and phenylpropanoid catabolic processes. Transcription factors (TFs) such as apetala2 /ethylene response factor ( AP2/ERF ) and Squamosa promoter binding protein-like ( SPL ) predicated in silico were validated by degradome sequencing. Association analysis of the DE miRNAs and transcriptome datasets identified that miR156 family targeted DHQ / SDH (3-dehydroquinate dehydratase/shikimate dehydrogenase), the key gene in the phenylpropanoid pathway. Conclusions: This study established comprehensive functional genomic resources for the CGA biosynthesis and provided insights into the molecular mechanisms involving in this process. The results also enabled the first perceptions of the regulatory roles of mRNAs and miRNAs and offered candidate genes for leafy sweet potato improvement s.

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“…In addition, the following downstream two enzyme genes, 4CL andCinnamate 4-hydroxylaseC4H),, which catalyzed the formation of p-Coumaroy-CoA, did not express significantly differently among comparisons in this reseach. These results were quite different from other higher plants, for them PAL, 4CL and C4H were key enzymes in CGA accumulation [44]. However, the intriguing phenomennon occured on three common DEGs HCT, CCR and CCoMAT in the phenylalanine pathway.…”

Section: Discussioncontrasting

confidence: 72%

Integrated transcriptome, small RNA and degradome sequencing approaches proffer insights into chlorogenic acid (CGA) biosynthesis in leafy sweet potato

Liu

¹

,

Su

²

,

Wang

³

et al. 2019

Preprint

0

View full text Add to dashboard Cite

Background: Phenolic compounds play key roles in health protection and leafy sweet potato is an excellent source of total phenolics (TP). The chlorogenic acid (CGA) family, which includes caffeoylquinic acid (CQA), 3,4-O-dicaffeoylquinic acid (3,4-diCQA), 3,5-O-dicaffeoylquinic acid (3,5-diCQA) and 4,5-O-dicaffeoylquinic acid (4,5-diCQA) , constitutes the major components of phenolic compounds in leafy sweet potato. However, the mechanism of CGA biosynthesis in leafy sweet potato is unclear. The objective of present study is to dissect the mechanisms of CGA biosynthesis by using transcriptome, small RNA (sRNA) and degradome sequencing. Results: Transcriptome sequencing of twelve samples (triple replicates) from one low-CGA content genotype and one high-CGA content genotype at two stages (65 and 85 days after planting) identified a total of 2333 common differentially expressed genes (DEGs). The enriched DEGs were related to photosynthesis, starch and sucrose metabolism and phenylpropanoid biosynthesis. In this study, functional genes CCR , CCoAOMT and HCT in the CGA biosynthetic pathway were uniformly downregulated, indicating the way to lignin was altered, and two possible CGA biosynthetic routes were hypothesized. The sRNA sequencing identified a total of 38 DE miRNAs. Using in silico approaches, 1799 targets were predicated for 38 DE miRNAs. The target genes were enriched in lignin and phenylpropanoid catabolic processes. Transcription factors (TFs) such as apetala2 /ethylene response factor ( AP2/ERF ) and Squamosa promoter binding protein-like ( SPL ) predicated in silico were validated by degradome sequencing. Association analysis of the DE miRNAs and transcriptome datasets identified that miR156 family targeted DHQ / SDH (3-dehydroquinate dehydratase/shikimate dehydrogenase), the key gene in the phenylpropanoid pathway. Conclusions: This study established comprehensive functional genomic resources for the CGA biosynthesis and provided insights into the molecular mechanisms involving in this process. The results also enabled the first perceptions of the regulatory roles of mRNAs and miRNAs and offered candidate genes for leafy sweet potato improvement s.

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“…Flavonoids in L. japonica were degressively abundant in leaves, flowers and stems, and leaves showed the highest antioxidative intensity than those of flowers or stems (Seo et al 2012). Tian found that phenolic acids in flowers and leaves were similar and both were higher than that in stems (Tian et al 2019), and these results could also be found in SYH (Chen et al 2015b;Yuan et al 2014). Meanwhile, powder of SYH leaves has already been used as dietary supplementation in animal diets (Long et al 2016).…”

Section: Ethnopharmacologymentioning

confidence: 98%

Lonicerae japonicae flos and Lonicerae flos: a systematic review of ethnopharmacology, phytochemistry and pharmacology

Li

¹

,

Li

²

,

Fu

³

et al. 2019

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Lonicerae japonicae flos (called Jinyinhua, JYH in Chinese), flowers or flower buds of Lonicera japonica Thunberg, is an extremely used traditional edible-medicinal herb. Pharmacological studies have already proved JYH ideal clinical therapeutic effects on inflammation and infectious diseases and prominent effects on multiple targets in vitro and in vivo, such as pro-inflammatory protein inducible nitric oxide synthase, toll-like receptor 4, interleukin-1 receptor. JYH and Lonicerae flos [called Shanyinhua, SYH in Chinese, flowers or flower buds of Lonicera hypoglauca Miquel, Lonicera confusa De Candolle or Lonicera macrantha (D.Don) Spreng]which belongs to the same family of JYH were once recorded as same herb in multiple versions of Chinese Pharmacopoeia (ChP). However, they were listed as two different herbs in 2005 Edition ChP, leading to endless controversy since they have close proximity on plant species, appearances and functions, together with traditional applications. In the past decades, there has no literature regarding to systematical comparison on the similarity concerning research achievements of the two herbs. This review comprehensively presents similarities and differences between JYH and SYH retrospectively, particularly proposing them the marked differences in botanies, phytochemistry and pharmacological activities which can be used as evidence of separate list of JYH and SYH. Furthermore, deficiencies on present studies have also been discussed so as to further research could use for reference.

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Transcriptome Analysis Reveals the Mechanism Underlying the Production of a High Quantity of Chlorogenic Acid in Young Leaves of Lonicera macranthoides Hand.-Mazz

Cited by 32 publications

References 42 publications

Integrated transcriptome, small RNA and degradome sequencing approaches proffer insights into chlorogenic acid (CGA) biosynthesis in leafy sweet potato

Integrated transcriptome, small RNA and degradome sequencing approaches proffer insights into chlorogenic acid (CGA) biosynthesis in leafy sweet potato

Integrated transcriptome, small RNA and degradome sequencing approaches proffer insights into chlorogenic acid (CGA) biosynthesis in leafy sweet potato

Lonicerae japonicae flos and Lonicerae flos: a systematic review of ethnopharmacology, phytochemistry and pharmacology

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