Transcriptome and Comparative Gene Expression Analysis of Sogatella furcifera (Horváth) in Response to Southern Rice Black-Streaked Dwarf Virus

Xu, Yi; Zhou, Wenwu; Zhou, Yijun; Wu, Jiajing; Zhou, Xueping

doi:10.1371/journal.pone.0036238

Cited by 87 publications

(98 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Of the SSRs, 21.09, 31.71, and 42.53% were mononucleotide, dinucleotide, and trinucleotide repeats, respectively (Figure 3). The results demonstrated that trinucleotide repeats were the most abundant microsatellites in this transcriptome data set, which is consistent with other recent reports in insects (Xu et al, 2012). Among all of the SSR types, AAC/GTT (21.58%) represented the dominant type, followed by A/T (21.02%), AG/CT (14.29%), AT/AT (8.53%), and AC/GT (8.32%) ( Table S4).…”

Section: Ssr Discoverysupporting

confidence: 93%

Transcriptome profiling of the crofton weed gall fly Procecidochares utilis

Gao¹,

Zhu²,

Ma³

et al. 2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Procecidochares utilis is a tephritid gall fly, which is known to be an effective biological agent that can be used to control the notoriously widespread crofton weed Eupatorium adenophorum. Despite its importance, genetic resources for P. utilis remain scarce. In this study, 1.2 Gb sequences were generated using Illumina pairedend sequencing technology. De novo assemblies yielded 491,760 contigs, 90,474 scaffolds, and 58,562 unigenes. Among the unigenes, 34,809 (59.44%) had a homologous match against the National Center for Biotechnology Information non-redundant protein database by translated Basic Local Alignment Search Tool (BlastX) with a cutoff E-value of 10 -5 . Among the unigenes, 57,627 were classified in the Gene Ontology database, 15,910 were assigned to Clusters of Orthologous Groups, and 38,565 were found in Kyoto Encyclopedia of Genes and Genomes. In addition, 5723 simple sequence repeats (SSRs) were discovered based on the unigene sequences. The transcriptome sequences and SSRs obtained represent a major molecular resource for P. utilis, which will extend our knowledge of the comparative and 2858 X. Gao et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 2857-2864 (2014) functional genomics of this organism and enable population genomic and gene-based association studies of the gall fly.

show abstract

Section: Ssr Discoverysupporting

confidence: 93%

Transcriptome profiling of the crofton weed gall fly Procecidochares utilis

Gao¹,

Zhu²,

Ma³

et al. 2014

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…The key components of the RNAi pathway are highly conserved within insect species (12). To obtain the sequences encoding the core components of the RNAi pathway in the transcriptome of SBPH or WBPH, we used a local BLASTN search with sequences of Nilaparvata lugens (JX023532.1, JX644040.1, KC316038.1, and JX644040.1 for DCR2, DCR1, AGO2, and AGO1, respectively) as queries (18,20,34). The SBPH DCR2 and AGO2 and WBPH AGO2 gene sequences were obtained from the GenBank (accession numbers JX023531.1, JX023533.1, and JX023535.1, respectively).…”

Section: Methodsmentioning

confidence: 99%

“…The plant reoviruses, plant rhabdoviruses, tospoviruses, and tenuiviruses are transmitted in a persistent propagative manner by sap-sucking insect vectors, including thrips, aphids, planthoppers, and leafhoppers (1)(2)(3). The key siRNA pathway factors DCR2 and AGO2 are conserved in sap-sucking insects that transmit persistent propagative plant viruses (15)(16)(17)(18)(19)(20). Indeed, the RNAi pathway is triggered by the replication of persistent propagative plant viruses in insect vectors (15)(16)(17)(18).…”

mentioning

confidence: 99%

“…The key siRNA pathway factors DCR2 and AGO2 are conserved in sap-sucking insects that transmit persistent propagative plant viruses (15)(16)(17)(18)(19)(20). Indeed, the RNAi pathway is triggered by the replication of persistent propagative plant viruses in insect vectors (15)(16)(17)(18). Thus, the similarities of virus-vector interactions of persistent propagative plant viruses and arboviruses suggest that the replication of plant viruses might also induce a siRNA antiviral response in insect vectors.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Small Interfering RNA Pathway Modulates Initial Viral Infection in Midgut Epithelium of Insect after Ingestion of Virus

Lan

Chen

Liu

et al. 2016

J Virol

View full text Add to dashboard Cite

IMPORTANCE Many viral pathogens that cause significant global health and agricultural problems are transmitted via insect vectors. The first bottleneck in viral infection, the midgut epithelium, is a principal determinant of the ability of an insect species to transmit a virus. Southern rice black streaked dwarf virus (SRBSDV) is restricted exclusively to the midgut epithelium of an incompetent vector, the small brown planthopper (SBPH).Here, we show that silencing of the core component Dicer-2 of the small interfering RNA (siRNA) pathway increases viral titers in the midgut epithelium past the threshold (1.99 ؋ 10 9 copies of the SRBSDV P10 gene/g of midgut RNA) for viral dissemination into the midgut muscles and then into the salivary glands, allowing the SBPH to become a competent vector of SRBSDV. This result is the first evidence that the siRNA antiviral pathway has a direct role in the control of viral dissemination from the midgut epithelium and that it affects the competence of the virus's vector. Many viral pathogens that cause significant global health and agricultural problems are arthropod borne and transmitted via an insect vector. Many plant viruses are transmitted in a persistent manner by sap-sucking insects, including planthoppers, leafhoppers, thrips, and aphids (1-3). Plant viruses, when ingested by their insect vectors, establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles (1-3). The viruses then move from the midgut muscles into the hemolymph and finally into the salivary glands, from where they can be introduced into plant hosts (1-3). Efficient viral infection of the midgut epithelium, where initial infection is established, is thus a determinative factor in the ability of an insect species to transmit a certain plant virus.This ability of an insect species to transmit a virus, which has been described as vector competence, is common in nature (4, 5). For many years, the midgut barrier has been believed to be the principal determinant of vector competence (1-9). For example, infection of the incompetent insect vectors of Rice dwarf virus and SRBSDV, two plant reoviruses, and Tomato spotted wilt virus (TSWV), a tospovirus, is restricted to the midgut epithelium (7-10). The inability of incompetent insect vectors to transmit these viruses may be caused by a failure of the virus to efficiently replicate in or disseminate from the midgut epithelium (1-5). It is possible that viral titers in the midgut epithelium of incompetent vectors are unable to reach the threshold needed for viral dissemination. The innate immune response in the midgut epithelium may effectively control viral accumulation, thus blocking viral transmission by incompetent vectors. However, whether the initial antiviral immune pathway(s) in the insect midgut can modulate the competence of plant virus vectors is not unknown.Several mosquito and Drosophila innate immune responses have been reported to have an antiviral effect. These responses include RNA interfere...

show abstract

“…In turn, viruses have evolved a large number of strategies to modulate the UPS process. Virus infection can affect the transcription levels of genes important for the UPS process (35,36). Alternatively, viruses also can modulate the UPS process by directly interacting with UPS components.…”

mentioning

confidence: 99%

Rice Stripe Tenuivirus Nonstructural Protein 3 Hijacks the 26S Proteasome of the Small Brown Planthopper via Direct Interaction with Regulatory Particle Non-ATPase Subunit 3

et al. 2015

J Virol

Self Cite

View full text Add to dashboard Cite

The ubiquitin/26S proteasome system plays a vital role in regulating host defenses against pathogens. Previous studies have highlighted different roles for the ubiquitin/26S proteasome in defense during virus infection in both mammals and plants, but their role in the vectors that transmit those viruses is still unclear. In this study, we determined that the 26S proteasome is present in the small brown planthopper (SBPH) (Laodelphgax striatellus) and has components similar to those in plants and mammals. There was an increase in the accumulation of Rice stripe virus (RSV) in the transmitting vector SBPH after disrupting the 26S proteasome, indicating that the SBPH 26S proteasome plays a role in defense against RSV infection by regulating RSV accumulation. Yeast two-hybrid analysis determined that a subunit of the 26S proteasome, named RPN3, could interact with RSV NS3. Transient overexpression of RPN3 had no effect on the RNA silencing suppressor activity of RSV NS3. However, NS3 could inhibit the ability of SBPH rpn3 to complement an rpn3 mutation in yeast. Our findings also indicate that the direct interaction between RPN3 and NS3 was responsible for inhibiting the complementation ability of RPN3. In vivo, we found an accumulation of ubiquitinated protein in SBPH tissues where the RSV titer was high, and silencing of rpn3 resulted in malfunction of the SBPH proteasome-mediated proteolysis. Consequently, viruliferous SBPH in which RPN3 was repressed transmitted the virus more effectively as a result of higher accumulation of RSV. Our results suggest that the RSV NS3 protein is able to hijack the 26S proteasome in SBPH via a direct interaction with the RPN3 subunit to attenuate the host defense response. IMPORTANCEWe show, for the first time, that the 26S proteasome components are present in the small brown planthopper and play a role in defense against its vectored plant virus (RSV). In turn, RSV encodes a protein that subverts the SBPH 26S proteasome via direct interaction with the 26S proteasome subunit RPN3. Our results imply that the molecular arms race observed in plant hosts can be extended to the insect vector that transmits those viruses. Rice stripe virus (RSV), one of the most destructive pathogens of rice production, has been responsible for numerous epidemics since it was first described in Japan in 1897 (1-3). RSV is the type member of the Tenuivirus genus, and the viral genome consists of four single-stranded RNA segments which range in size from approximately 8.9 to 2.1 kb (4, 5). RNA 1 is negative sense and encodes a putative RNA-dependent RNA polymerase. RNAs 2, 3, and 4 are ambisense, and each contains two open reading frames (ORFs), with one on the viral RNA strand (vRNA) and the second on the viral cRNA strand (vcRNA) (6). RSV vRNA 2 encodes a membrane-associated protein that reportedly is an RNA silencing suppressor and interacts with suppressor of gene silencing 3 (SGS3) (7). vcRNA 2 encodes a glycoprotein (NSvc2) which when expressed in insect cells is displayed on the membrane su...

show abstract

Transcriptome and Comparative Gene Expression Analysis of Sogatella furcifera (Horváth) in Response to Southern Rice Black-Streaked Dwarf Virus

Cited by 87 publications

References 64 publications

Transcriptome profiling of the crofton weed gall fly Procecidochares utilis

Transcriptome profiling of the crofton weed gall fly Procecidochares utilis

Small Interfering RNA Pathway Modulates Initial Viral Infection in Midgut Epithelium of Insect after Ingestion of Virus

Rice Stripe Tenuivirus Nonstructural Protein 3 Hijacks the 26S Proteasome of the Small Brown Planthopper via Direct Interaction with Regulatory Particle Non-ATPase Subunit 3

Contact Info

Product

Resources

About