Transcriptome and functional responses to absence of astaxanthin in Atlantic salmon fed low marine diets

Ytrestøyl, Trine; Afanasyev, Sergey; Ruyter, Bente; Hatlen, Bjarne; Østbye, Tone-Kari K; Krasnov, Aleksei

doi:10.1016/j.cbd.2021.100841

Cited by 10 publications

(19 citation statements)

References 87 publications

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“…It is well known that supplementation of Ax reduced lipid peroxidation (Karppi et al 2007;Ni et al 2015). Interestingly, Ytrestøyl et al (2021) also observed a modulation of hmox1 in liver, which in this case, revealed an up-regulation in salmon fed Ax. Nevertheless, based on our results, Ax would therefore impact redox balance also through heme-oxygenase-related pathway (Niu et al 2018;Wang and Zhuang 2019).…”

Section: Ax Effect In Liver Of Post-smolt Salmon + Ax Vs − Axsupporting

confidence: 64%

“…The up-regulation of fbp1 may be potentially due to intracellular glucose uptake leading to either activation of the futile cycle to regulate the glycolysis or activation of gluconeogenesis due to a decrease of glucose in the blood (Newsholme and Crabtree 1970;Jung and Sikora 1984;Rakus et al 2005;Garrett and Grisham 2010). We can notice that the study of Ytrestøyl et al (2021) also showed a modulation of the motility-related coronin gene but was found to be down-regulated in salmon fed Ax. Pooley et al (2013) studied the recombinant interleukin 1 beta (IL-1β)-induced inflammatory response of primary muscle cell cultures in Atlantic salmon and demonstrates the modulation of the expression of immune-related genes including the down-regulation of TNF alpha gene and the activation of the gene encoding Jun transcription factor.…”

Section: Ax Effect In Muscle Of Post-smolt Salmon + Ax Vs − Axmentioning

confidence: 79%

“…The authors hypothesized that Ax may influence the resistance of rainbow trout to viral pathogen via inhibition of virally induced and ROS-mediated oxidative stress. Ytrestøyl et al (2021) also showed some inflammation effects in salmon without Ax. However, the modulated genes observed were not the same as in our study, as they described, up-regulations of ladderlectin and gimaps genes, or genes responsive to virus, such as trim39-like genes in salmon fed Ax.…”

Section: Effect Of Astaxanthin On Pylorus Of Post-smolt Salmon (+ Ax Vs − Ax)mentioning

confidence: 89%

“…In muscle, the heatmap confirms that the main DEG pattern occurred before and after smoltification. Looking only at the effect of Ax supplementation in post-smolt salmon, Ytrestøyl et al (2021) observed the highest number of DEG muscle. This difference could potentially be due to the level of Ax in feed which is lower in Ytrestøyl et al (2021) than in the present study.…”

Section: Differentially Expressed Genes and Transcriptome Clusteringmentioning

confidence: 93%

“…Microarray analyses have already been performed in Atlantic salmon brain, gills, kidney, and proximal intestine to evaluate the effect of smoltification or seawater transfer on the host transcriptome and have suggested effects on growth, metabolism, oxygen transport (Seear et al 2010), and the immune functions of Atlantic salmon (Johansson et al 2016). Very recently, Ytrestøyl et al (2021) published a study of transcriptome-based data from Ax-free post smolt Atlantic salmon compared to Ax-fed post-smolt Atlantic salmon. This study showed that the intestinal and muscle transcriptomes were more affected than the liver.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Mechanism Involved in Carotenoid Metabolism in Post-Smolt Atlantic Salmon: Astaxanthin Metabolism During Flesh Pigmentation and Its Antioxidant Properties

et al. 2021

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A better understanding of carotenoid dynamics (transport, absorption, metabolism, and deposition) is essential to develop a better strategy to improve astaxanthin (Ax) retention in muscle of Atlantic salmon. To achieve that, a comparison of postsmolt salmon with (+ Ax) or without (− Ax) dietary Ax supplementation was established based on a transcriptomic approach targeting pyloric, hepatic, and muscular tissues. Results in post-smolts showed that the pyloric caeca transcriptome is more sensitive to dietary Ax supplementation compared to the other tissues. Key genes sensitive to Ax supplementation could be identified, such as cd36 in pylorus, agr2 in liver, or fbp1 in muscle. The most modulated genes in pylorus were related to absorption but also metabolism of Ax. Additionally, genes linked to upstream regulation of the ferroptosis pathway were significantly modulated in liver, evoking the involvement of Ax as an antioxidant in this process. Finally, the muscle seemed to be less impacted by dietary Ax supplementation, except for genes related to actin remodelling and glucose homeostasis. In conclusion, the transcriptome data generated from this study showed that Ax dynamics in Atlantic salmon is characterized by a high metabolism during absorption at pyloric caeca level. In liver, a link with a potential of ferroptosis process appears likely via cellular lipid peroxidation. Our data provide insights into a better understanding of molecular mechanisms involved in dietary Ax supplementation, as well as its beneficial effects in preventing oxidative stress and related inflammation in muscle.

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Section: Ax Effect In Liver Of Post-smolt Salmon + Ax Vs − Axsupporting

confidence: 64%

Section: Ax Effect In Muscle Of Post-smolt Salmon + Ax Vs − Axmentioning

confidence: 79%

Section: Effect Of Astaxanthin On Pylorus Of Post-smolt Salmon (+ Ax Vs − Ax)mentioning

confidence: 89%

Section: Differentially Expressed Genes and Transcriptome Clusteringmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Molecular Mechanism Involved in Carotenoid Metabolism in Post-Smolt Atlantic Salmon: Astaxanthin Metabolism During Flesh Pigmentation and Its Antioxidant Properties

et al. 2021

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Improved intestinal absorption and oral bioavailability of astaxanthin using poly (ethylene glycol)‐graft‐chitosan nanoparticles: preparation, in vitro evaluation, and pharmacokinetics in rats

Zhu

Liao

et al. 2021

J Sci Food Agric

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BACKGROUND: Astaxanthin (ASTA) is a kind of food-derived active ingredient (FDAI) with antioxidant and antidiabetic functions. It is nontoxic but its poor solubility and low bioavailability hinder its application in the food industry. In this study, a novel carrier, polyethylene glycol-grafted chitosan (PEG-g-CS) was applied to enhance the bioavailability of astaxanthin. It encapsulated astaxanthin completely by solvent evaporation to manufacture astaxanthin using poly (ethylene glycol)-graftchitosan nanoparticles (ASTA-PEG-g-CS) nanoparticles to improve absorption. RESULTS:The ASTA-PEG-g-CS nanoparticles were spherical, with a particle size below 200 nm and a ζ potential of about −26 mV. Polyethylene glycol-grafted chitosan can encapsulate astaxanthin well, and the encapsulated astaxanthin was released rapidlyin 15 min in an in vitro release study. In a rat single-pass intestinal perfusion study, a low concentration of ASTA-PEG-g-CS nanoparticle (0.2 ∼g mL −1 ) was better absorbed in the intestine. In particular, the jejunum could absorb most astaxanthin without a change in the concentration. An in vivo release study also demonstrated that ASTA-PEG-g-CS nanoparticles enhanced oral bioavailability significantly.CONCLUSION: This novel carrier, PEG-g-CS, provided a simple way to encapsulate food, which improved the bioavailability of hydrophobic ingredients.

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CO2-equivalent emissions and quality evaluation of chilled and frozen Atlantic salmon transported from Norway to Japan

Redo,

Che,

Tolstorebrov

et al. 2024

International Journal of Refrigeration

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Transcriptome and functional responses to absence of astaxanthin in Atlantic salmon fed low marine diets

Cited by 10 publications

References 87 publications

Molecular Mechanism Involved in Carotenoid Metabolism in Post-Smolt Atlantic Salmon: Astaxanthin Metabolism During Flesh Pigmentation and Its Antioxidant Properties

Molecular Mechanism Involved in Carotenoid Metabolism in Post-Smolt Atlantic Salmon: Astaxanthin Metabolism During Flesh Pigmentation and Its Antioxidant Properties

Improved intestinal absorption and oral bioavailability of astaxanthin using poly (ethylene glycol)‐graft‐chitosan nanoparticles: preparation, in vitro evaluation, and pharmacokinetics in rats

CO2-equivalent emissions and quality evaluation of chilled and frozen Atlantic salmon transported from Norway to Japan

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