Transcriptome-based analysis on carbon metabolism of Haematococcus pluvialis mutant under 15% CO 2

Li, Ke; Cheng, Jun; Lu, Hongxiang; Yang, Weijuan; Zhou, Junhu; Cen, Kefa

doi:10.1016/j.biortech.2017.02.121

Cited by 52 publications

(30 citation statements)

References 38 publications

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“…From the TEM images, more lipid drops were observed when using nonmotile cells as seeds. Cheng reported that massive astaxanthin was esterified in the endoplasmic reticulum, and deposits in cytoplasmic lipid droplets to avoid the feedback inhibitor of carotenoids biosynthesis (Cheng et al 2017). Thus, the result confirmed the better photoinduction result of non-motile cells.…”

Section: Resultssupporting

confidence: 52%

“…Compared with control, the transcription level of pk gene was 1.3-fold up-regulated at the end of photoinduction (Table 1). Chen also reported that the increase of esterified form astaxanthin synthesis was accompanied by a 3.5-fold increase in pk (Cheng et al 2017). Acetyl-CoA is the precursor for lipid synthesis.…”

Section: Transcriptome and Pathway Analysis Involved In Pyruvate Metamentioning

confidence: 92%

“…Transcriptome sequencing was regarded as an efficient approach for exploring the mechanism of astaxanthin accumulation. The gene transcription changes of H. pluvialis have been discussed in many studies, e.g., high light intensity could increase the activity of β-carotene ketolase (BKT) and IPP isomerase (Linden 1999); astaxanthin synthesis-related genes were significantly up-regulated in H. pluvialis mutant under 15% CO 2 (Li et al 2017); the astaxanthin accumulation ability of H. pluvialis could be improved by gibberellin (GA3), accompanied by increasing the transcription of ipi (isopentenyl pyrophosphate isomerase), psy (phytoene synthase), pds (phytoene desaturase) and bkt genes (Gao et al 2013). However, these researches were mainly focused on the regulation mechanism of astaxanthin synthesis responding to various stresses, e.g., light, salinity, temperature, hormone, iron and inorganic carbon (Lee et al 2016;Wen et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Pyruvate metabolism plays an important role in glycolysis to form acetyl-CoA. Cheng et al (2017) reported that most significant differences were found in unigenes related to photosynthesis, carotenoid biosynthesis and fatty acid biosynthesis pathways when photoinduction with high light under 15% CO 2 . Above studies indicated astaxanthin synthesis mechanism is complicated and has not been systematically clarified.…”

Section: Introductionmentioning

confidence: 99%

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Astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism

Fang

Zhang

Fei

et al. 2020

Bioresour. Bioprocess.

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Background: Haematococcus pluvialis is the best source of natural astaxanthin, known as the king of antioxidants. H. pluvialis have four cell forms: spore, motile cell, non-motile cell and akinete. Spores and motile cells are susceptible to photoinhibition and would die under photoinduction conditions. Photoinduction using non-motile cells as seeds could result in a higher astaxanthin production than that using akinetes. However, the mechanism of this phenomenon has not been clarified. Results:Transcriptome was sequenced and annotated to illustrate the mechanism of this phenomenon. All differentially expressed genes involved in astaxanthin biosynthesis were up-regulated. Particularly, chyb gene was up-regulated by 16-fold, improving the conversion of β-carotene into astaxanthin. Pyruvate was the precursor of carotenoids biosynthesis. Pyruvate kinase gene expression level was increased by 2.0-fold at the early stage of akinetes formation. More changes of gene transcription occurred at the early stage of akinetes formation, 52.7% and 51.9% of total DEGs in control group and treatment group, respectively. Conclusions:Genes transcription network was constructed and the synthesis mechanism of astaxanthin was clarified. The results are expected to further guide the in-depth optimization of the astaxanthin production process in H. pluvialis by improving pyruvate metabolism.

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Section: Resultssupporting

confidence: 52%

Section: Transcriptome and Pathway Analysis Involved In Pyruvate Metamentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism

Fang

Zhang

Fei

et al. 2020

Bioresour. Bioprocess.

View full text Add to dashboard Cite

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“…In general, KEGG enrichment analysis of the DEGs identi ed many transcripts that were associated with the biosynthesis of primary and secondary metabolites, photosynthesis, and immune system responses. And these pathways were consistent with the roles of the stress resistance system in plants [5,[15][16][17][18][19][20]. The results indicated that the regulations subjected by H. pluvialis response to SAHL stresses changed along with transition of the treatment stages.…”

Section: Rna Sequencing Assembly and Annotation Analysissupporting

confidence: 78%

Transcriptome-based analysis of the effects of salicylic acid and high light on lipid and astaxanthin accumulation in Haematococcus pluvialis

Huang

et al. 2020

Preprint

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Background: The fresh water unicellular alga Haematococcus pluvialis has achieved considerable interests for its capacity to accumulate large amounts of triacylglycerol and astaxanthin under various environmental stresses. To our knowledge, works focusing on transcriptome research of H. pluvialis under exogenous hormones together with physical stresses is rare.Results: In the present study, the change patterns at transcriptome level were analyzed to distinguish the multiple defensive systems of astaxanthin and fatty acid metabolism against exogenous salicylic acid and high light (SAHL) stresses. Based on RNA-seq data, a total of 112,463 unigenes and 61,191 genes were annotated in six databases, including NR, KEGG, Swiss-Prot, PFAM, COG and GO. The top five categorized GO terms based on these genes were “catalytic activity”, “binding”, “cellular process”, “cell” and “cell part”. Furthermore, 705 unigenes predicted as putative transcription factors (TFs) were identified and assigned to 25 families. The top five most abundant TFs families were C3H, MYB, Nin-like, MTB_related and ERF, which were likely to be associated with the biosynthesis of astaxanthin and fatty acid in H. pluvialis upon exposure to the SAHL stresses. Analysis of differentially expressed genes (DEGs) in KEGG identified many transcripts that associated with the biosynthesis of primary and secondary metabolites, photosynthesis, and immune system responses. Additionally, fifteen key genes that involved in astaxanthin and fatty acid biosynthesis pathways presented the same transcriptional pattern in response to the SAHL stresses, and positive correlations were observed among them by Pearson Correlation (PC) analysis, indicating the coordination between astaxanthin and fatty acid biosynthesis. Furthermore, protein–protein interaction (PPI) network analysis also demonstrated that this coordination might be at transcriptional level.Conclusion: The results demonstrated in this study provide valuable information to illustrate the molecular mechanisms of coordinate relations between astaxanthin and fatty acid biosynthesis, which may play a role in both genetic and metabolic engineering efforts to improve astaxanthin and fatty acid production by H. pluvialis.

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Transcriptome Differential Analysis of Chlorella sp. Cultivated with Simulated Flue Gases

Fan

Ren

2019

Env Prog and Sustain Energy

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Transcriptome sequencing and annotation was performed on Chlorella sp. cultivated with simulated gases (15% (v/v) CO2‐air aeration) with addition of four groups of dilute gases including 1500 mL SO2 (50 and 200 ppm) and 1500 mL NO2 (50 and 200 ppm) correspondingly to demonstrate why the biomass and the protein increase but the content of fatty acid has a little drop with NO2–CO2‐air aeration. Differential expressed genes analysis indicated that including 89 upregulated and 1443 downregulated unigenes in the algae when cultivated with 15% CO2–SO2 (200 ppm, 1500 mL)‐air aeration and 93 upregulated and 1776 downregulated unigenes in the algae when cultivated with 15% CO2–NO2 (200 ppm, 1500 mL)‐air aeration were identified. The genes involved in TCA cycle of carbon metabolic pathway were almost downregulated. The genes which encoded the three enzymes including long‐chain fatty acid CoA ligase, alcohol dehydrogenase, and aldehyde dehydrogenase (NAD+) were upregulated 1.15, 1.00, and 1.06‐fold in N group and 1.15, 1.94, and 0.88‐fold downregulated in S group, respectively, in fatty acid degradation pathway. However, the genes involved in three enzyme including nitrate/nitrite transporter, nitrite reductase, and nitrate reductase were upregulated 4.63, 2.85, and 7.00‐folded in N group and downregulated 1.67, 1.53, and 1.65‐folded in S group, respectively, in nitrate assimilation process. © 2019 American Institute of Chemical Engineers Environ Prog, 38: e13217, 2019

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Transcriptome-based analysis on carbon metabolism of Haematococcus pluvialis mutant under 15% CO 2

Cited by 52 publications

References 38 publications

Astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism

Astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism

Transcriptome-based analysis of the effects of salicylic acid and high light on lipid and astaxanthin accumulation in Haematococcus pluvialis

Transcriptome Differential Analysis of Chlorella sp. Cultivated with Simulated Flue Gases

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