Transcriptome-based discovery of AP2/ERF transcription factors related to temperature stress in tea plant (Camellia sinensis)

Wu, Zhijun; Li, Xinghui; Liu, Zhiwei; Li, Hui; Wang, Yongxin; Zhuang, Jing

doi:10.1007/s10142-015-0457-9

Cited by 80 publications

(48 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In summary, the RPKM values revealed that the four tea plant cultivars differed in the expression of CsNAC genes. These tea plant cultivars are grown in different regions of China [39, 40], and the differences may occur on the individual evolution of different cultivars.…”

Section: Resultsmentioning

confidence: 99%

“…The completed transcriptome sequencing of tea plant provides an opportunity to identify gene families at the transcriptome level [39]. Recent studies have identified and analyzed 89 AP2/ERF, 50 WRKY, 16 HSF, and 18 bZIP genes in tea plant; the roles of these genes in response to adversity stresses were also elucidated [40–43]. To date, detailed analysis of NAC family genes in tea plant remains to be conducted.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptome-Wide Identification and Expression Analysis of the NAC Gene Family in Tea Plant [Camellia sinensis (L.) O. Kuntze]

Wang

Liu

et al. 2016

PLoS ONE

Self Cite

View full text Add to dashboard Cite

In plants, the NAC (NAM-ATAF1/2-CUC) family of proteins constitutes several transcription factors and plays vital roles in diverse biological processes, such as growth, development, and adaption to adverse factors. Tea, as a non-alcoholic drink, is known for its bioactive ingredients and health efficacy. Currently, knowledge about NAC gene family in tea plant remains very limited. In this study, a total of 45 CsNAC genes encoding NAC proteins including three membrane-bound members were identified in tea plant through transcriptome analysis. CsNAC factors and Arabidopsis counterparts were clustered into 17 subgroups after phylogenetic analysis. Conserved motif analysis revealed that CsNAC proteins with a close evolutionary relationship possessed uniform or similar motif compositions. The distribution of NAC family MTFs (membrane-associated transcription factors) among higher plants of whose genome-wide has been completed revealed that the existence of doubled TMs (transmembrane motifs) may be specific to fabids. Transcriptome analysis exhibited the expression profiles of CsNAC genes in different tea plant cultivars under non-stress conditions. Nine CsNAC genes, including the predicted stress-related and membrane-bound genes, were examined through qRT-PCR (quantitative real time polymerase chain reaction) in two tea plant cultivars, namely, ‘Huangjinya’ and ‘Yingshuang’. The expression patterns of these genes were investigated in different tissues (root, stem, mature leaf, young leaf and bud) and under diverse environmental stresses (drought, salt, heat, cold and abscisic acid). Several CsNAC genes, including CsNAC17 and CsNAC30 that are highly orthologous to known stress-responsive ANAC072/RD26 were identified as highly responsive to abiotic stress. This study provides a global survey of tea plant NAC proteins, and would be helpful for the improvement of stress resistance in tea plant via genetic engineering.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome-Wide Identification and Expression Analysis of the NAC Gene Family in Tea Plant [Camellia sinensis (L.) O. Kuntze]

Wang

Liu

et al. 2016

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…The regulation of Dof TFs in response to abiotic stress has been investigated in other plants. Some researchers have also analyzed other TFs families in tea plant [34, 35]. There is, however, the regulatory mechanism of Dof TFs in tea plant which is not completely understood till now.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome-Based Analysis of Dof Family Transcription Factors and Their Responses to Abiotic Stress in Tea Plant (Camellia sinensis)

Huang

Liu

et al. 2016

International Journal of Genomics

Self Cite

View full text Add to dashboard Cite

Tea plant (Camellia sinensis (L.) O. Kuntze) is affected by abiotic stress during its growth and development. DNA-binding with one finger (Dof) transcription factors (TFs) play important roles in abiotic stress tolerance of plants. In this study, a total of 29 putative Dof TFs were identified based on transcriptome of tea plant, and the conserved domains and common motifs of these CsDof TFs were predicted and analyzed. The 29 CsDof proteins were divided into 7 groups (A, B1, B2, C1, C2.1, C2.2, and D2), and the interaction networks of Dof proteins in C. sinensis were established according to the data in Arabidopsis. Gene expression was analyzed in “Yingshuang” and “Huangjinya” under four experimental stresses by qRT-PCR. CsDof genes were expressed differentially and related to different abiotic stress conditions. In total, our results might suggest that there is a potential relationship between CsDof factors and tea plant stress resistance.

show abstract

“…ERFs are members of one of the largest transcription factor families, which is considered a superfamily, and is involved in plant biological and abiotic stress responses (Licausi et al, 2013;Min et al, 2012;M€ uller and Munn e-Bosch, 2015;Phukan et al, 2017). It has been reported that CsAP2/ERF(APETALA2/ERF) may play important roles in the abnormal temperature stress response in Camellia sinensis (Wu et al, 2015). TERF2/LeERF2 enhance the freezing tolerance of Nicotiana tabacum and Solanum lycopersicum through ethylene biosynthesis and the ethylene signaling pathway (Zhang and Huang, 2010).…”

mentioning

confidence: 99%

Expression of Ethylene Response Factor Genes during Fresh-cut Lotus Root Storage and Browning

Min¹,

Xie²,

Yi³

et al. 2018

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Ethylene response factor (ERF) genes have been characterized in numerous plants in which they are involved in responses to biotic and abiotic stress, including cold and heat stress. Cool temperatures is one of the most effective storage methods for delaying browning of fresh-cut lotus (Nelumbo nucifera) root. In model plants, ERF genes have been identified as being responsive to cold and heat stress. Whether ERF is associated with lotus root browning in cooler temperatures has not been studied. In this research, low-temperature storage (4 8C) effectively delayed browning of fresh-cut lotus root. Using RNA sequencing, seven Nelumbo nucifera ERF (NnERF) genes were isolated and studied. Transcriptional analysis indicated NnERF genes responded differently to temperature. NnERF3/4/5 were reduced continuously by a low temperature (4 8C) and NnERF5 was the most strongly downregulated. In contrast, transcripts of NnERF1/2/7 were increased at a low temperature (4 8C). The expression of NnERF6 showed no obvious difference between the two different temperatures. It is proposed that NnERF3/4/5 could be important candidates as regulators of fresh-cut lotus root browning. The roles of other members are also discussed.

show abstract

Transcriptome-based discovery of AP2/ERF transcription factors related to temperature stress in tea plant (Camellia sinensis)

Cited by 80 publications

References 63 publications

Transcriptome-Wide Identification and Expression Analysis of the NAC Gene Family in Tea Plant [Camellia sinensis (L.) O. Kuntze]

Transcriptome-Wide Identification and Expression Analysis of the NAC Gene Family in Tea Plant [Camellia sinensis (L.) O. Kuntze]

Transcriptome-Based Analysis of Dof Family Transcription Factors and Their Responses to Abiotic Stress in Tea Plant (Camellia sinensis)

Expression of Ethylene Response Factor Genes during Fresh-cut Lotus Root Storage and Browning

Contact Info

Product

Resources

About