Transcriptome profile and association study revealed<i>STAT3</i>gene as a potential quality marker of bovine gametes

Ghanem, Nasser; Salilew‐Wondim, Dessie; Hoelker, M.; Schellander, K.; Tesfaye, Dawit

doi:10.1017/s0967199419000765

Cited by 6 publications

(6 citation statements)

References 66 publications

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“…While, a candidate gene encodes for protein that functions, as zinc transporter (SLC39A8) is upregulated in cumulus cells of bovine [26] and mice oocytes [27]. Recently, STAT3 was identifi ed as a candidate gene of oocyte developmental potential in a study done in our lab [28].…”

Section: Methods Used For Oocyte Selectionmentioning

confidence: 99%

Cumulus-oocyte developmental competence: From morphological selection to molecular markers

Ghanem¹,

Samy²,

Ahmed³

et al. 2020

J Gynecol Res Obstet

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The term "oocyte competence or quality" is the fi nal measurement of its ability to achieve all cytoplasmic, nuclear and molecular events either in vivo or in vitro to bring a healthy offspring. Sirard, et al. [1] have defi ned the levels of competence as the ability of oocyte to resume meiosis, cleave following fertilization, develop and differentiate into blastocyst stage, induce pregnancy and fi nally bring healthy offspring.

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Section: Methods Used For Oocyte Selectionmentioning

confidence: 99%

Cumulus-oocyte developmental competence: From morphological selection to molecular markers

Ghanem¹,

Samy²,

Ahmed³

et al. 2020

J Gynecol Res Obstet

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“…regulates IFN expression in the developing blastocyst [38]); prostaglandin-endoperoxide synthase 2 (PTGS2), which is expressed in the embryo trophectoderm and plays a role in implantation [39]; Actin Alpha 2 (ACTA2), a molecular marker, which in bovine, is associated with embryo developmental competence [40]; Placenta Associated 8 (PLAC8), which is involved in placental development [41]; Signal transducer and activator of transcription 3 (STAT3), a quality marker for bovine gametes [42] and is involved in inner cell mass development in bovine blastocysts [43]; Sex determining region Y-box 2 (SOX2), a marker for bovine inner cell mass, which is involved in the developmental capacity regulation of the embryo [44]; POU domain, class 5 transcription factor 1 (OCT4), which is expressed at early developmental stages and is involved in the differentiation of the inner cell mass and the trophectoderm layers [45]; DNA methyltransferase 1 (DNMT1) plays a role in the maintenance of hemi-methylated strands during DNA replication [46] and has an essential role during bovine preimplantation development [47]; DNA methyltransferase 3B (DNMT3B) is essential for de novo methylation [48]; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ) and succinate dehydrogenase complex flavoprotein subunit A (SDHA) served as internal reference genes [28].…”

Section: Plos Onementioning

confidence: 99%

Carryover effects of feeding bulls with an omega-3-enriched-diet—From spermatozoa to developed embryos

et al. 2022

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The impact of omega-3 nutritional manipulation on semen cryosurvival and quality post thawing is controversial. Our aim was to examine how feeding bulls with omega-3 supplementation from different sources affects the spermatozoa quality parameters. Fifteen Israeli Holstein bulls were fed for 13 weeks with a standard ration top-dressed with encapsulated-fat supplementation: fish or flaxseed oil or saturated fatty acids (control). Ejaculates were collected before, during, and after the feeding trial. Frozen–thawed samples were evaluated by a flow cytometer for spermatozoa viability, mitochondrial membrane potential, the level of reactive oxygen species (ROS), acrosome membrane integrity, DNA fragmentation, phosphatidylserine translocation, and membrane fluidity. Both fish and flaxseed oil treatment resulted in lower ROS levels vs. control groups, during and after the feeding trial. Fewer spermatozoa with damaged acrosomes were observed in the fish oil group after the feeding trial. The spermatozoa membrane fluidity was altered in both the fish and flaxseed oil groups throughout the feeding trial, but only in the flaxseed oil group after the feeding trial. The proportion of spermatozoa with fragmented DNA was lower in the flaxseed oil group after the feeding trial. The spermatozoa fertilization competence did not differ between groups however, blastocyst formation rate was higher in the fish and flaxseed oil groups relative to the control. This was associated with differential gene expression in the blastocysts. Overall, the omega-3-enriched food improved the spermatozoa characteristics; this was further expressed in the developing blastocysts, suggesting a carryover effect from the spermatozoa to the embryos.

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“…Cells viability was measured using trypan blue and quality was estimated using intracellular reactive oxygen species successively on day 3 after heat treatment and day 7. Moreover, metabolic activity was performed by measuring the mitochondrial activity and transcriptional activity was done by profiling four selected candidate genes (HSF1, StAR, and P53, BCL2) using quantitative real-time PCR (Ghanem et al, 2020b).…”

Section: Experimental Designmentioning

confidence: 99%

“…The reverse transcription of RNA samples to cDNA was done using RevertAid first-strand cDNA synthesis kit (ThermoFisher Scientific, USA) according to Ghanem et al (2020b). The following chemicals were added to each of RNA samples, 1 μl of oligo dt18 primer, 4 μl of PCR buffer, 2 μl of dNTPs, 1 μl of RNase inhibitor, 1 μl RNase inhibitor enzyme, 1 μl of reverse transcriptase enzyme were gently mixed by pipetting.…”

Section: The Synthesis Of Cdnamentioning

confidence: 99%

Transcriptional, Mitochondrial Activity, and Viability of Egyptian Buffalo’s Granulosa Cells In Vitro Cultured under Heat Elevation

Ghanem¹,

Faheem²,

Samy³

et al. 2021

WVJ

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It is documented that heat stress caused impairment on the reproductive performance of dairy animals. However, there are few reports that have focused on the molecular and intracellular responses of in vitro cultured buffalo granulosa cells during heat elevation. The present study was conducted to investigate the effect of heat elevation during in vitro culture of buffalo granulosa cells on their viability, quality, mitochondrial activity, and transcriptional activity. Granulosa cells were harvested after aspiration of cumulus-oocytes complexes that were collected from abattoir ovaries. The granulosa cells were cultured in vitro either at a normal physiological temperature suitable for oocyte maturation and embryo development (38.5°C) or exposed to the elevated temperature of 40.5°C on day 3 of culture (the first two days were for confluence) for two hours of culture then continued at 38.5°C up to day 7 of culture. The viability of granulosa cells was measured using trypan blue and quality was estimated by measuring the level of intracellular reactive oxygen species (ROS) on day 7. Moreover, metabolic activity was performed by measuring the fluorescent intensity of mitochondria. Moreover, transcriptional activity was done by profiling four selected candidate genes using quantitative real-time PCR. The results indicated that the granulosa cells viability rate significantly decreased in the heat stress group (25.1 ± 3.7), compared to the control group (36.6 ± 5.3) on confluence day (day 3). In addition, the viability rate on the last day of culture (day 7) decreased in heat stress, compared to control (83.7 ± 4.5 and 97.4 ± 0.4, respectively). On the other hand, there was a nonsignificant difference in ROS profile between the control (21.7*104 ± 1.3) and the heat-stressed group (15.7 ± 0.7) on day 7 of culture. However, the mitochondrial fluorescent intensity was higher in the control (21.9 ± 1.9) than in the heat-stressed group (15.4 ± 0.8) on day 7 of culture. The expression of cellular defense (HSF1) and apoptosis-inducing gene (P53) were significantly up-regulated in granulosa cells exposed to heat elevation, compared to the control group. On the other hand, the steroidogenesis-regulating gene (StAR) was down-regulated in granulosa cells cultured under heat shock, compared to the control group. In conclusion, heat stress reduced the viability of granulosa cells by inducing the expression of an apoptosis-related gene (P53) and compromised expression of genes regulating the steroid biosynthesis, which resulted in up-regulation of cell defense gene (HSF1) in an attempt to ameliorate the deleterious effect of heat stress on the biological activity of the granulosa cells.

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Transcriptome profile and association study revealedSTAT3gene as a potential quality marker of bovine gametes

Cited by 6 publications

References 66 publications

Cumulus-oocyte developmental competence: From morphological selection to molecular markers

Cumulus-oocyte developmental competence: From morphological selection to molecular markers

Carryover effects of feeding bulls with an omega-3-enriched-diet—From spermatozoa to developed embryos

Transcriptional, Mitochondrial Activity, and Viability of Egyptian Buffalo’s Granulosa Cells In Vitro Cultured under Heat Elevation

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