Transcriptome profile of skeletal muscle at different developmental stages in Large White and Mashen pigs

Guo, Xiaohong; Zhang, Wanfeng; Li, Meng; Gao, Pengfei; Hei, Wei; He, Zhiqiang; Wu, Yiqi; Liu, Juan; Cai, Chunbo; Cao, Guoqing

doi:10.1139/cjas-2019-0002

Cited by 4 publications

(3 citation statements)

References 53 publications

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“…GNAS connects to the Gap junction pathway, which encodes Gsα, XLαs, NESP55, and A/B protein. It has been identified as a candidate gene related to skeletal muscle growth and development in Chinese native pig breeds [ 36 ], and it may regulate daily gain, feed conversion rate, carcass traits, and other important economic traits [ 37 ]. Imumorin et al also verified this point in cattle [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Population Structure Analysis and Genetic Diversity Detection of Four Chinese Indigenous Duck Breeds from Fujian Province

Lin¹,

Li²,

Yang³

et al. 2022

Animals

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The assessment of population genetic structure is the basis for understanding the genetic information of indigenous breeds and is important for the protection and management of indigenous breeds. However, the population genetic differentiation of many local breeds still remains unclear. Here, we performed a genome-wide comparative analysis of Jinding, Liancheng white, Putian black, and Shanma ducks based on the genomic sequences using RAD sequencing to understand their population structure and genetic diversity. The population parameters showed that there were obvious genetic differences among the four indigenous breeds, which were separated groups. Among them, Liancheng white and Shanma ducks may come from the same ancestor because the phylogenetic tree forms three tree trunks. In addition, during the runs of homozygosity (ROH), we found that the average inbreeding coefficient of Liancheng white and Putian black ducks was the lowest and the highest, respectively. Five genomic regions were considered to be the hotspots of autozygosity among these indigenous duck breeds, and the candidate genes involved a variety of potential variations, such as muscle growth, pigmentation, and neuroregulation. These findings provide insights into the further improvement and conservation of Fujian duck breeds.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Population Structure Analysis and Genetic Diversity Detection of Four Chinese Indigenous Duck Breeds from Fujian Province

Lin¹,

Li²,

Yang³

et al. 2022

Animals

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“…Skeletal muscle is an energetically demanding tissue that accounts for 40-50% of body mass [7,8]. Muscle is composed of a heterogenous population of muscle fibers that are 2 of 19 classified by contractile speed (slow-twitch or fast-twitch) and metabolism (glycolytic or oxidative).…”

Section: Introductionmentioning

confidence: 99%

Skeletal Muscle Metabolism Is Dynamic during Porcine Postnatal Growth

Rimmer,

Geisbrecht,

Chao

et al. 2024

Metabolites

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Skeletal muscle metabolism has implications for swine feed efficiency (FE); however, it remains unclear if the metabolic profile of skeletal muscle changes during postnatal growth. To assess the metabolic changes, samples were collected from the longissimus dorsi (LD, glycolytic muscle), latissimus dorsi (LAT, mixed muscle), and masseter (MS, oxidative muscle) at 20, 53, 87, 120, and 180 days of age from barrows. Muscles were assessed to determine the abundance of several metabolic enzymes. Lactate dehydrogenase (LDHα) decreased in all muscles from 20 to 87 d (p < 0.01), which may be attributed to the muscles being more glycolytic at weaning from a milk-based diet. Pyruvate carboxylase (PC) increased in all muscles at 53 d compared to the other time points (p < 0.01), while pyruvate dehydrogenase α 1 (PDHα1) increased at 87 and 180 d in MS compared to LD (p < 0.05), indicating that potential changes occur in pyruvate entry into the tricarboxylic acid (TCA) cycle during growth. Isolated mitochondria from each muscle were incubated with 13C-labeled metabolites to assess isotopomer enrichment patterns of TCA intermediates. Citrate M + 2 and M + 4 derived from [13C3]-pyruvate increased at 87 d in LAT and MS mitochondria compared to LD mitochondria (p < 0.05). Regardless of the muscle, citrate M+3 increased at 87 d compared to 20, 53, and 120 d, while 180 d showed intermediate values (p < 0.01). These data support the notion that pyruvate metabolism is dynamic during growth. Our findings establish a metabolic fingerprint associated with postnatal muscle hypertrophy.

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“…Based on the transcriptome sequencing results of longissimus dorsi muscle in different developmental stages of Mashen and Large White pigs, the differentially expressed CAVIN3 gene was screened, which was speculated to play a role in regulating fat metabolism in pigs (Guo et al, 2019). Therefore, this study focused on the function of CAVIN3 and explored the regulatory effect of CAVIN3 on the adipogenic differentiation of porcine preadipocytes.…”

Section: Introductionmentioning

confidence: 99%

Caveolae‐associated protein 3 promotes adipogenic differentiation of porcine preadipocytes by promoting extracellular signal‐regulated kinase phosphorylation

Shi

Yang

Huang

et al. 2023

Animal Science Journal

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Fat deposition is one of the key factors affecting the economic development of pig husbandry. The aim of this study was to investigate the expression characteristics of caveolae‐associated protein 3 (CAVIN3) and to elucidate its effect and mechanism on adipogenic differentiation of porcine preadipocytes. Cell transfection, quantitative reverse transcription polymerase chain reaction (qRT‐PCR), western blot, and oil red O staining were used to detect the effect of CAVIN3 on the differentiation of porcine preadipocytes. The results showed that CAVIN3 was expressed in various tissues, with higher expression in adipose tissue, differentially expressed during cell adipogenic differentiation, and mainly distributed in the cytoplasm. Functional studies showed that, after CAVIN3 interference in preadipocytes, the expression of adipogenic factors and the content of lipid droplets were significantly decreased (p < 0.05). The results were reversed after CAVIN3 was overexpressed. The mechanism research showed that LY3214996 inhibited the extracellular signal‐regulated kinase (ERK) phosphorylation and further inhibited lipogenic factors expression. Overexpression of CAVIN3 attenuates the inhibitory effect of LY3214996 on ERK phosphorylation and attenuates its inhibitory effect on adipogenic differentiation. Therefore, this study demonstrated that CAVIN3 promotes the differentiation of porcine preadipocytes by promoting ERK phosphorylation. The present study can lay a theoretical foundation for further studying the molecular mechanism of porcine fat deposition.

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Transcriptome profile of skeletal muscle at different developmental stages in Large White and Mashen pigs

Cited by 4 publications

References 53 publications

Genome-Wide Population Structure Analysis and Genetic Diversity Detection of Four Chinese Indigenous Duck Breeds from Fujian Province

Genome-Wide Population Structure Analysis and Genetic Diversity Detection of Four Chinese Indigenous Duck Breeds from Fujian Province

Skeletal Muscle Metabolism Is Dynamic during Porcine Postnatal Growth

Caveolae‐associated protein 3 promotes adipogenic differentiation of porcine preadipocytes by promoting extracellular signal‐regulated kinase phosphorylation

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