Transcriptome Profiling Analyses in Psoriasis: A Dynamic Contribution of Keratinocytes to the Pathogenesis

Rioux, Geneviève; Ridha, Zainab; Simard, Marie; Turgeon, Florence; Guérin, Sylvain L.; Pouliot, Roxane

doi:10.3390/genes11101155

Cited by 38 publications

(22 citation statements)

References 126 publications

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“…There is also a localized overexpression of IFI27 and PI3 in the suprabasal layer. Some of these results complement previous findings obtained by bulk microarray, GWAS, and NGS methods [56,59,74,75]. Such examples include the AD-like loss of differentiation signature from the top epidermal layers (FLG, LOR, KRT10, KRT1) and a loss of expression of genes involved in lipid metabolism (EVOLV3, FABP5) [74].…”

Section: Psoriasissupporting

confidence: 82%

Challenges and Opportunities for the Translation of Single-Cell RNA Sequencing Technologies to Dermatology

Ascensión

Araúzo-Bravo

Izeta

2022

Life

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Skin is a complex and heterogeneous organ at the cellular level. This complexity is beginning to be understood through the application of single-cell genomics and computational tools. A large number of datasets that shed light on how the different human skin cell types interact in homeostasis—and what ceases to work in diverse dermatological diseases—have been generated and are publicly available. However, translation of these novel aspects to the clinic is lacking. This review aims to summarize the state-of-the-art of skin biology using single-cell technologies, with a special focus on skin pathologies and the translation of mechanistic findings to the clinic. The main implications of this review are to summarize the benefits and limitations of single-cell analysis and thus help translate the emerging insights from these novel techniques to the bedside.

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Section: Psoriasissupporting

confidence: 82%

Challenges and Opportunities for the Translation of Single-Cell RNA Sequencing Technologies to Dermatology

Ascensión

Araúzo-Bravo

Izeta

2022

Life

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“…The transcriptome of psoriasis skin has been extensively explored and compared with both non-lesional skin and skin from healthy controls [ 24 , 25 , 26 ], but not much attention has been drawn to the blood transcriptome in psoriasis. Recently, however, blood transcriptomic profiling of patients with psoriasis vs. controls identified inflammasome signaling as the most upregulated canonical pathway in psoriasis and found a common gene transcript signature related to neutrophil-driven inflammation [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Neutrophil Pathways of Inflammation Characterize the Blood Transcriptomic Signature of Patients with Psoriasis and Cardiovascular Disease

Kvist-Hansen

Kaiser

Wang

et al. 2021

IJMS

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Background: Patients with psoriasis have an increased risk of atherosclerotic cardiovascular disease (CVD). The molecular mechanisms behind this connection are not fully understood, but the involvement of neutrophils have drawn attention as a shared inflammatory factor. Methods: RNA sequencing using the Illumina platform was performed on blood from 38 patients with moderate to severe psoriasis; approximately half had prior CVD. The neutrophil to lymphocyte ratio (NLR) was obtained from blood samples. Subclinical atherosclerosis was assessed by 18F-fluorodeoxyglucose positron emission tomography/computed tomography and ultrasound imaging. Transcriptomic analysis for differential expression and functional enrichment were performed, followed by correlation analyses of differentially expressed genes (DEGs), NLR and subclinical measurers of CVD. Results: 291 genes were differentially expressed between patients with psoriasis with and without CVD. These included 208 upregulated and 83 downregulated DEGs. Neutrophil degranulation was identified as the most significant process related to the upregulated DEGs. Genes for the neutrophil-associated markers MPO, MMP9, LCN2, CEACAM1, CEACAM6 and CEACAM8 were identified as being of special interest and their mRNA levels correlated with NLR, high-sensitive C-reactive protein and markers of subclinical CVD. Conclusions: Patients with psoriasis and CVD had an increased expression of genes related to neutrophil degranulation in their blood transcriptome compared with patients with psoriasis without CVD. NLR may be a potential biomarker of subclinical CVD in psoriasis.

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“…Two opposite biological processes (herbivorous diet leading to GPR15–C10ORF99 signalling and colonic Treg homing, and no herbivorous diet over a long time period, leading to the loss of colonic C10ORF99 expression) suggested that colonic Treg homing reactions can be promoted by an herbivorous diet, but can be disrupted by the lack of an herbivorous diet over a long time period, leading to disappearance of the colon and the loss of a functional C10ORF99 gene. In addition, the mRNA expression of C10ORF99 was also induced in skin in response to epithelial damage and TLR signalling (Kiene et al , 2014 ; Rioux et al , 2020 ), and is highly upregulated in skin during inflammatory diseases such as psoriasis (Suply et al , 2017 ; Chen et al , 2018 ).…”

Section: Discussionmentioning

confidence: 99%

GPR15–C10ORF99 functional pairing initiates colonic Treg homing in amniotes

et al. 2021

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Regulatory T lymphocyte (Treg) homing reactions mediated by G protein-coupled receptor (GPCR)-ligand interactions play a central role in maintaining intestinal immune homeostasis by restraining inappropriate immune responses in the gastrointestinal tract. However, the origin of Treg homing to the colon remains mysterious. Here, we report that the C10ORF99 peptide (also known as CPR15L and AP57), a cognate ligand of GPR15 that controls Treg homing to the colon, originates from a duplication of the flanking CDHR1 gene and is functionally paired with GPR15 in amniotes. Evolutionary analysis and experimental data indicate that the GPR15-C10ORF99 pair is functionally conserved to mediate colonic Treg homing in amniotes and their expression patterns are positively correlated with herbivore diet in the colon. With the first herbivorous diet in early amniotes, a new biological process (herbivorous diet short-chain fatty acid-C10ORF99/GPR15-induced Treg homing colon immune homeostasis) emerged, and we propose an evolutionary model whereby GPR15-C10ORF99 functional pairing has initiated the first colonic Treg homing reaction in amniotes. Our findings also highlight that GPCR-ligand pairing leads to physiological adaptation during vertebrate evolution.

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Transcriptome Profiling Analyses in Psoriasis: A Dynamic Contribution of Keratinocytes to the Pathogenesis

Cited by 38 publications

References 126 publications

Challenges and Opportunities for the Translation of Single-Cell RNA Sequencing Technologies to Dermatology

Challenges and Opportunities for the Translation of Single-Cell RNA Sequencing Technologies to Dermatology

Neutrophil Pathways of Inflammation Characterize the Blood Transcriptomic Signature of Patients with Psoriasis and Cardiovascular Disease

GPR15–C10ORF99 functional pairing initiates colonic Treg homing in amniotes

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