Transcriptome profiling in psoriasis: NB-UVB treatment-associated transcriptional changes and modulation of autoinflammation in perilesional skin in early-phase disease

Vacharanukrauh, Pinyadapat; Meephansan, Jitlada; Ponnikorn, Saranyoo; Tangtanatakul, Pattarin; Soonthornchai, Wipasiri; Wongpiyabovorn, Jongkonnee; Ingkaninanda, Patlada; Akimichi, Morita

doi:10.1016/j.jdermsci.2022.08.004

Cited by 10 publications

(12 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…NB-UVB treatment suppresses serum levels of psoriasis-driving cytokines, such as tumor necrosis factor (TNF)-α, IL-8, IL-12, IL-17, IL-22, IL-23, and IL-34 and elevates the levels of IL-10, an anti-inflammatory cytokine, in patients with psoriasis 14,15 . Moreover, NB-UVB treatment significantly suppresses the mRNA levels of nuclear factor-kappa-B inhibitor zeta, serpin family B member 4 (SERPINB4), autophagy related 13, and cathepsin S in the edges of psoriatic plaques 16 and may attenuate TLR-3/4/9 activity in psoriatic plaques 17 .…”

mentioning

confidence: 99%

Elucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques

Boonpethkaew

Meephansan

Charoensuksira

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

Narrow band-ultraviolet B (NB-UVB) is an effective treatment for psoriasis. We aim to generate a potential mechanism of NB-UVB through comparing the transcriptomic profile before and after NB-UVB treatment between the peripheral edge of lesional skin (PE skin) and the center of lesional skin (CE skin) on the basis of molecular mechanisms of these two areas display different downstream functions. More than one-fourth of the NB-UVB-altered genes were found to be plaque-specific. Some of them were psoriasis signature genes that were downregulated by NB-UVB in, both, PE and CE skin (core alteration), such as IL36G, DEFB4A/B, S100A15, KRT16, and KRT6A. After NB-UVB treatment, the activity score of upstream cytokines, such as interferons, interleukin (IL)-6, IL-17, and IL-22 in pathogenesis decreased. In addition, NB-UVB could restore normal keratinization by upregulating LORICRIN and KRT2, particularly in the CE skin. Finally, we illustrated that NB-UVB is capable of suppressing molecules from the initiation to maintenance phase of plaque formation, thereby normalizing psoriatic plaques. This finding supports the usefulness of NB-UVB treatment in clinical practice and may help in the development of new treatment approaches in which NB-UVB treatment is included for patients with psoriasis or other inflammatory skin diseases.

show abstract

mentioning

confidence: 99%

Elucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques

Boonpethkaew

Meephansan

Charoensuksira

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…They concluded UVB-mediated phototherapy works partially through SIRT1 anti-inflammatory effects downregulating NF-kB. All patients showed clinical improvement their lesions after therapy ( 161 ). As mentioned previously NAM is rapidly converted to NAD + in cells through sirtuin deacetylation activity.…”

Section: Wound Healingmentioning

confidence: 97%

“…Psoriasis is another chronic inflammatory disease mediated through the activation of T cells which stimulate the proliferation of keratinocyte. Applications of sirtuins in psoriasis have been extensively studied (149,151,(157)(158)(159)(160). In a recent study D' Amico and colleagues, took punch biopsies from 6 untreated female patients affected with chronic plaque psoriasis.…”

Section: Human Disease Models and Clinical Applicationsmentioning

confidence: 99%

The role of sirtuins in dermal fibroblast function

et al. 2023

View full text Add to dashboard Cite

The sirtuins are a family of seven proteins that perform a variety of dermatological functions and help maintain both the structure and function of the skin. More specifically, the sirtuins have been shown to be altered in multiple dermal cell types including dermal fibroblasts. The functions of dermal fibroblasts are extensive, and include playing a significant role in wound healing as well as helping to maintain the integrity of the skin. As dermal fibroblasts age, they can undergo a state of permanent cell cycle arrest, known as cellular senescence. This senescent process can occur as a result of various stressors, including oxidative stress, ultraviolet radiation -induced stress, and replicative stress. In recent years, there has been a growing interest in both enhancing the cutaneous fibroblast’s ability to facilitate wound healing and altering fibroblast cellular senescence. Thus, in this review, we examine the relationship between sirtuin signaling and dermal fibroblasts to understand how this family of proteins may modulate skin conditions ranging from the wound healing process to photocarcinogenesis associated with fibroblast senescence. Additionally, we offer supporting data from experiments examining the relationship between fibroblast senescence and sirtuin levels in an oxidative stress model indicating that senescent dermal fibroblasts exhibit diminished sirtuin levels. Furthermore, we survey the research on the role of sirtuins in specific dermatological disease states that where dermal fibroblast function has been implicated. Finally, we conclude with outlining potential clinical applications of sirtuins in dermatology. In sum, we find that the literature on the involvement of sirtuins in dermal fibroblasts is limited, with research still in its early stages. Nevertheless, intriguing preliminary findings merit additional investigation into the clinical implications of sirtuins in dermatology.

show abstract

“…Representing ~9% of total UVR in sunlight 19 , UVB can suppress primary and anamnestic responses and result in antigen specific tolerance 20 . UVB exposure has therapeutic value in the treatment of psoriasis via modulation of autoinflammatory pathways 21 , has been shown to affect metabolic programming and senescence in keratinocytes 22,23 , can drive transcriptional changes associated with photo-aging 24 and can induce epigenetic changes during carcinogenesis 25 ). In models of cutaneous leishmaniasis (CL), UVB has varied effects depending on parasite species and/or level of UVB exposure 26,27 .…”

Section: Graphical Abstract 45 Introductionmentioning

confidence: 99%

UVB modifies skin immune-stroma cross-talk and promotes effector T cell recruitment during crypticLeishmania donovaniinfection

Dey

Bocxlaer

et al. 2023

Preprint

View full text Add to dashboard Cite

Many parasites of significant public health importance assume skin residency without causing overt pathlogy. How immune and stromal cells respond to such cryptic infections and how exposure to UVB alters such responses in poorly understood. We combined scRNA-seq, spatial transcriptomics and inferential network analysis to address these questions in a model of cryptic skin infection by Leishmania donovani. In infected C57BL/6 mice, p-selectin and CXCL12 interactions dominate intercellular communication between leucocytes, fibroblast and endothelial cells, but effector T cell function remains muted. Following UVB exposure, increased numbers of IFNgamma+ CD4+ Th1 cells and NK cells enter the skin, communicating with stromal cells via CCL5-CCR5 and LFA-1-ICAM1/2. However, spatial mapping indicated that Th1 cells and macrophages occupied distinct niches after UVB exposure, likely limiting effector function. Our data provide the first holistic view of the immune landscape during cryptic L. donovani infection and demonstrate how UVB exposure fundamentally reshapes this response.

show abstract

Transcriptome profiling in psoriasis: NB-UVB treatment-associated transcriptional changes and modulation of autoinflammation in perilesional skin in early-phase disease

Cited by 10 publications

References 49 publications

Elucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques

Elucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques

The role of sirtuins in dermal fibroblast function

UVB modifies skin immune-stroma cross-talk and promotes effector T cell recruitment during crypticLeishmania donovaniinfection

Contact Info

Product

Resources

About