Transcriptome profiling of plerocercoid and adult developmental stages of the neglected medical tapeworm Spirometra erinaceieuropaei

Liu, Shi Nan; Su, Xiao; Chen, Wen Qing; Yu, Jin Hai; Li, Jing Ru; Jiang, Peng; Cui, Jing; Wang, Zhong Quan; Zhang, Xi

doi:10.1016/j.actatropica.2022.106483

Cited by 14 publications

(27 citation statements)

References 58 publications

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“…Genes encoding proteins that contain the glutathione transferase domain in the S. mansoni were searched using the NCBI conserved domains database. All candidate SmGSTs were obtained from the WormBase ParaSite database and the transcriptomic data ( 6 ). These extracted sequences were identified belong to the GST family by querying for genes annotated with the Pfam domain accessions PF02798, PF14497 and PF00043 for cytosolic GSTs, and PF01124 for MAPEG.…”

Section: Methodsmentioning

confidence: 99%

“…These publicly available datasets offer opportunities to perform detailed analysis of the GST family in Spirometra tapeworms. More importantly, previous genomic and transcriptomic analysis showed highly expressed of GST genes in S. mansoni, indicating important roles of GST in the life cycle of Spirometra tapeworms (6,19). Therefore, understanding the structure, molecular characteristics and evolutionary pattern of the GST family will be helpful for understanding the interactions between parasite and host, as well as developing new intervention strategies for sparganosis.…”

Section: Introductionmentioning

confidence: 98%

“…Although several methods have been introduced in diagnosis and treatment, precision medicine for sparganosis still has a long way to go (5). Understanding the intricacies of the parasite and its interactions with hosts is central to developing new intervention strategies (6). Several parasite molecules have been implicated in the interference with the host response, such as the glutathione transferases (GSTs), which is a super family of enzymes found ubiquitously in organisms (7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, the first draft genome of S. mansoni was sequenced ( 19 ), and the expressed sequence tags (ESTs) were functionally analyzed ( 20 ). Recently, a phosphoproteomic analysis and a transcriptomic analysis of S. mansoni were performed ( 6 , 21 ). These publicly available datasets offer opportunities to perform detailed analysis of the GST family in Spirometra tapeworms.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characteristics of glutathione transferase gene family in a neglect medical Spirometra tapeworm

Chen

Liu²,

Cheng³

et al. 2022

Front. Vet. Sci.

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The Spirometra mansoni is a neglect medical tapeworm, its plerocercoid larvae can parasitize in humans and animals, causing sparganosis. In this study, 17 new members of the glutathione transferase (GST) family were sequenced and characterized in S. mansoni. Clustering analysis displayed the categorization of SmGSTs into two main clades. RT-qPCR illustrated that 7 GST genes were highly expressed in the plerocercoid stage while 8 GSTs were highly expressed in the adult. rSmGST has the typical C- and N-terminal double domains of glutathione transferase. Immunolocalization revealed that natural SmGST is mainly located in the epidermis and parenchyma of plerocercoid, and in the epidermis, parenchyma, uterus and egg shell of adult worm. The optimum activity for rSmGST was found to be pH 6.5 and 25°C. The evolutionary tree showed a high level of diversity of cestodes GSTs. SmGSTs contained both conserved family members and members in the process of further diversification. The findings in this study will lay a foundation to better explore the underlying mechanisms of GSTs involved in Spirometra tapeworms.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular characteristics of glutathione transferase gene family in a neglect medical Spirometra tapeworm

Chen

Liu²,

Cheng³

et al. 2022

Front. Vet. Sci.

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“…2 ). The first step was to analyze the transcriptome data ( https://www.ncbi.nlm.nih.gov/bioproject/PRJNA761840 ) [ 31 ] to uncover genome-wide SSRs using the MISA-web tool [ 32 ]. The search was carried out using the following parameters: (i) > 9 repeat units for mononucleotide microsatellites; (ii) ≥ 4 repeat units for di-, tri-nucleotide microsatellites; (iii) ≥ 3 repeat units for tetra-, penta- and hexa-nucleotide microsatellites; and (iv) repeat length up to 100 bp.…”

Section: Methodsmentioning

confidence: 99%

Genetic structure of Spirometra mansoni (Cestoda: Diphyllobothriidae) populations in China revealed by a Target SSR-seq method

Chen

Liu

et al. 2022

Parasites Vectors

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Background In China, the plerocercoid of the cestode Spirometra mansoni is the main causative agent of human and animal sparganosis. However, the population genetic structure of this parasite remains unclear. In this study, we genotyped S. mansoni isolates with the aim to improve current knowledge on the evolution and population diversity of this cestode. Methods We first screened 34 perfect simple sequence repeats (SSRs) using all available omic data and then constructed target sequencing technology (Target SSR-seq) based on the Illumina NovaSeq platform. Next, a series of STRUCTURE. clustering, principal component, analysis of molecular variance and TreeMix analyses were performed on 362 worm samples isolated from 12 different hosts in 16 geographical populations of China to identify the genetic structure. Results A total of 170 alleles were detected. The whole population could be organized and was found to be derived from the admixture of two ancestral clusters. TreeMix analysis hinted that possible gene flow occurred from Guizhou (GZ) to Sichuan (SC), SC to Jaingxi (JX), SC to Hubei (HB), GZ to Yunnan (YN) and GZ to Jiangsu (JS). Both neighbor-joining clustering and principal coordinate analysis showed that isolates from intermediate hosts tend to cluster together, while parasites from definitive hosts revealed greater genetic differences. Generally, a S. mansoni population was observed to harbor high genetic diversity, moderate genetic differentiation and a little genetic exchange among geographical populations. Conclusions A Target SSR-seq genotyping method was successfully developed, and an in-depth view of genetic diversity and genetic relationship will have important implications for the prevention and control of sparganosis. Graphical Abstract

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Identification of a Clinical Spirometra mansoni Plerocercoid Isolate Using Molecular and Morphological Data

Ru,

Cheng,

Jiang

et al. 2024

Acta Parasit.

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Transcriptome profiling of plerocercoid and adult developmental stages of the neglected medical tapeworm Spirometra erinaceieuropaei

Cited by 14 publications

References 58 publications

Molecular characteristics of glutathione transferase gene family in a neglect medical Spirometra tapeworm

Molecular characteristics of glutathione transferase gene family in a neglect medical Spirometra tapeworm

Genetic structure of Spirometra mansoni (Cestoda: Diphyllobothriidae) populations in China revealed by a Target SSR-seq method

Identification of a Clinical Spirometra mansoni Plerocercoid Isolate Using Molecular and Morphological Data

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