Transcriptome Profiling Reveals a Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Yellow Drum, Nibea albiflora

Abstract

The yellow drum (Nibea albiflora) is an important marine economic fish that is widely distributed in the coastal waters of the Northwest Pacific. In order to understand the molecular regulatory mechanism of the yellow drum under salinity stress, in the present study, transcriptome analysis was performed under gradients with six salinities (10, 15, 20, 25, 30, and 35 psu). Compared to 25 psu, 907, 1109, 1309, 18, and 243 differentially expressed genes (DEGs) were obtained under 10, 15, 20, 30, and 35 psu salinities, respectively. The differential gene expression was further validated by quantitative real-time PCR (qPCR). The results of the tendency analysis showed that all DEGs of the yellow drum under salinity fluctuation were mainly divided into three expression trends. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that the PI3K-Akt signaling pathway, Jak-STAT signaling pathway as well as the glutathione metabolism and steroid biosynthesis pathways may be the key pathways for the salinity adaptive regulation mechanism of the yellow drum. G protein-coupled receptors (GPCRs), the solute carrier family (SLC), the transient receptor potential cation channel subfamily V member 6 (TRPV6), isocitrate dehydrogenase (IDH1), and fructose-bisphosphate aldolase C-B (ALDOCB) may be the key genes in the response of the yellow drum to salinity stress. This study explored the transcriptional patterns of the yellow drum under salinity stress and provided fundamental information for the study of salinity adaptability in this species.

The Effect of Salinity Stress on Enzyme Activities, Histology, and Transcriptome of Silver Carp (Hypophthalmichthys molitrix)

“…Accordingly, it was not surprising to see a marked enrichment of glutathione metabolism under salinity stress. This result is consistent with those for Nibea albiflora [ 71 ].…”

“…The salinity stress response mechanism of fish involves numerous stress signal transduction pathways working synergistically [ 71 ]. Cell signal transduction is the stimulation and acceptance of the stimulation process, cell membrane, or cell receptor as mediated, which impacts the biological function of the cell.…”

Transcriptome analysis of Sparidentex hasta larvae exposed to water-accommodated fraction of Kuwait crude oil

“…The involvement of the PI3K-Akt pathway further highlights the complexity of the cellular response. This pathway is central to regulating cell survival and growth, and its activation may indicate an effort to maintain cellular homeostasis under the stress of crude oil exposure 65 – 69 . The upregulation of genes such as synaptosomal-associated protein and cytochrome P450 1B1 indicates its possible role in synaptic vesicle trafficking and neurotransmitter release, suggesting that crude oil exposure may influence neural processes in fish larvae.…”

Network-based integration of omics, physiological and environmental data in real-world Elbe estuarine Zander

“…While zander larvae are stenohaline bound to freshwater and oligohaline areas, juveniles acquire the capacity to adapt to a wider range of osmotic uctuations. Osmoregulation requires synergistic action of a complex sensing and signal transduction network that could be achieved by enhanced osmosensing in the juvenile zander via mitogen-activated protein kinase (MAPK) pathway [73] and PI3k-Akt regulating involved salinity stress response [74]. Further, we found cell-cell/ECM interactions related to gill remodeling processes during salinity uctuations [75], [76], [77] and protein tyrosine kinase 2 (here ptk2aa) as hub gene in gill remodeling [78].…”