Transcriptome Profiling Reveals the Antitumor Mechanism of Polysaccharide from Marine Algae Gracilariopsis lemaneiformis

Kang, Yani; Li, Hua; Wu, Jun; Xu, Xiaoting; Sun, Xue; Zhao, Xiaodong; Xu, Nianjun

doi:10.1371/journal.pone.0158279

Cited by 32 publications

(12 citation statements)

References 27 publications

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“…Thus, EVO influenced the expression of genes related to apoptosis and cell cycle. The effects of EVO on Caki-1 cells were similar to those observed for polysaccharides on a non-small cell lung cancer cell line 35 .…”

Section: Discussionsupporting

confidence: 68%

Cytological Assessments and Transcriptome Profiling Demonstrate that Evodiamine Inhibits Growth and Induces Apoptosis in a Renal Carcinoma Cell Line

Yuan

Zhang

Liu

et al. 2017

Sci Rep

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Chinese medicines are an important source of secondary metabolites with excellent antitumour activity. Evodia rutaecarpa, from the family Rutaceae, exhibits antitumour activity. Evodiamine (EVO), which was isolated from the fruit of E. rutaecarpa, exhibits robust antitumour activity. However, the antitumour mechanism of EVO remains unclear. In this study, we assessed the growth-inhibiting effect of EVO on two renal carcinoma cell lines. We found that EVO could change the morphology and decrease the viability and proliferation of cells in a time- and concentration-dependent manner in vitro. In addition, transcriptome analysis indicated that EVO can modulate the transcriptome of Caki-1 cells. In total, 7,243 differentially expressed genes were found, among which 3,347 downregulated genes and 3,896 upregulated genes were mainly involved in cell migration, apoptosis, cell cycle, and DNA replication. Furthermore, we demonstrated that EVO can cause apoptosis, arrest cells in the G2/M phase, and regulate the expression of apoptosis- and cell cycle-related genes in Caki-1 cells. Our study reveals the anticancer effects of EVO using cellular and molecular data, and indicates the potential uses of this compound as a resource to characterize the antitumour mechanisms of E. rutaecarpa.

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Section: Discussionsupporting

confidence: 68%

Cytological Assessments and Transcriptome Profiling Demonstrate that Evodiamine Inhibits Growth and Induces Apoptosis in a Renal Carcinoma Cell Line

Yuan

Zhang

Liu

et al. 2017

Sci Rep

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“…This confirms the results of previous studies that cembratriene-4, 6-diol has antitumor activity [23]. Thus, the effects of α-CBD on HepG2 and SMMC-7721 cells were similar to those of other substances in a cancer cell line [15,24,25]. The above results showed that the substances with antitumor activity similar to that of α-CBD exert antitumor activity by inhibiting the proliferation of tumor cells.…”

Section: Discussionsupporting

confidence: 90%

Anti-Tumor Activity of Cembranoid-Type Diterpenes Isolated from Nicotiana tabacum L.

Yuan

Mao

et al. 2019

Biomolecules

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Recently, the incidence of hepatocellular carcinoma has increased worldwide. Cembranoid-type diterpenes (CBDs) from tobacco exhibit good antimicrobial, antitumor, and neuroprotective activities. Therefore, in this study, we isolated CBDs from Nicotiana tabacum L. and evaluated their antitumor activity against hepatoma cell lines. Particularly, the anti-tumor activity of α-2,7,11-cyprotermine-4,6-diol (α-CBD) was investigated against HepG2, SMMC-7721, and HL-7702 cells. The MTT assay revealed that α-CBD reduced the formation of cell clones and inhibited the proliferation of hepatocellular carcinoma cells. Morphological observations showed that α-CBD altered cell morphology and membrane permeability before inducing apoptosis. To further explore the antitumor mechanism of α-CBD, flow cytometry and transcriptome analysis were performed using HepG2 cells. The results showed that the number of HepG2 cells increased from 10.4% to 29.8%, indicating that α-CBD inhibits the proliferation of hepatocellular carcinoma cells in the S phase. The gene expression analysis of HepG2 cells treated with α-CBD showed 3068 genes with altered expression, among which 1289 were upregulated and 1779 were downregulated. Apoptosis induced by these differentially expressed genes might be mediated by the p53-PUMA, PI3K-Akt, and IL-1-NF-κB-IAP pathways. Comprehensively, our study shows that α-CBD isolated from N. tabacum L. can be potentially used as a natural antitumor agent.

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“…We previously showed that PGL significantly inhibits lung cancer cell proliferation and changes cell morphology [ 12 ]. Moreover, our transcriptome analysis demonstrated that PGL induced lung cancer apoptosis and cell cycle arrest by modulating the expression of related genes [ 13 ]. In this study, we further investigated PGL antitumor activity in the human gastric cancer cell line MKN28, the lung cancer cell line A549, and the mouse melanoma cell line B16 using CCK-8 assays, phase-contrast microscopy, annexin V-FITC/PI staining, flow cytometry, RT-qPCR, western blotting, and transfections.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Potential Antitumor Activity of Polysaccharide from Gracilariopsis lemaneiformis

et al. 2017

Self Cite

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Substances with valuable antitumor properties have been identified in many marine algae, including an edible polysaccharide from the marine alga Gracilariopsis lemaneiformis (PGL). We previously reported transcriptome profiling data showing that PGL induced transcriptional alterations generate anti-lung cancer activity. To identify how PGL is detrimental to tumors, we purified PGL to characterize its chemical composition, molecular weight, and sugar and protein content and investigated its antitumor activity. We demonstrated that PGL exerted its antitumor activities by modulating cell viability, morphology, apoptosis, and the apoptosis-related Fas/FasL signaling pathway in the human lung cancer cell line A549, the gastric cancer cell line MKN28, and the mouse melanoma cell line B16. Our data provide the first evidence that PGL inhibits cell proliferation by inducing apoptosis, which is largely mediated by Fas/FasL in cancer cells, suggesting that PGL might be a novel therapeutic agent against cancer.

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Transcriptome Profiling Reveals the Antitumor Mechanism of Polysaccharide from Marine Algae Gracilariopsis lemaneiformis

Cited by 32 publications

References 27 publications

Cytological Assessments and Transcriptome Profiling Demonstrate that Evodiamine Inhibits Growth and Induces Apoptosis in a Renal Carcinoma Cell Line

Cytological Assessments and Transcriptome Profiling Demonstrate that Evodiamine Inhibits Growth and Induces Apoptosis in a Renal Carcinoma Cell Line

Anti-Tumor Activity of Cembranoid-Type Diterpenes Isolated from Nicotiana tabacum L.

Characterization and Potential Antitumor Activity of Polysaccharide from Gracilariopsis lemaneiformis

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