Transcriptome sequencing of <i>Salvia miltiorrhiza</i> after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis

Jiang, Yan; Wang, Lei; Lu, Shaorong; Xue, Yi-Zhe; Wei, Xiying; Lü, Juan; Zhang, Yanyan

doi:10.1080/13880209.2019.1680706

Cited by 26 publications

(14 citation statements)

References 68 publications

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“…Among these three, the SmCPS and SmHMGR2 genes were verified experimentally by RT-PCR studies in transgenic plants (Szymczyk et al 2016(Szymczyk et al , 2018. Similarly, the S. miltiorrhiza genes SmIDI, SmGGPPS, SmHMGS, SmHDR and SmDXR have also been experimentally-confirmed to respond to SA treatment (Hua et al 2012;Hao et al 2013Hao et al , 2015Jiang et al 2019;Yan et al 2009;Zhang et al 2010Zhang et al , 2016. SmDXS2 gene responded positively to overexpression of SmWRKY1, that is stimulated by SA treatment (Cao et al 2018).…”

Section: Distribution Of Sa Responsive Cis-active Elements Within Promentioning

confidence: 96%

Elicitation of solid callus cultures of Salvia miltiorrhiza Bunge with salicylic acid and a synthetic auxin (1-naphthaleneacetic acid)

Szymczyk

Szymańska

Kochan

et al. 2021

Plant Cell Tiss Organ Cult

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The presented study analyses the influence of salicylic acid (SA) and the synthetic auxin 1-naphthaleneacetic acid (NAA) on total tanshinone level and on dihydrotanshinone (DHT), cryptotanshinone (CT), tanshinine I (TI) and tanshinone IIA (TIIA) level in Salvia miltiorrhiza callus cultures growing on solid Murashige and Skoog (MS) medium. The influence of SA and NAA was evaluated at 10-day intervals throughout a 80-day treatment period. SA was applied at 0.1, 0.2 and 0.4 mM, and NAA at 2.69, 13.43, 26.85 and 40.28 μM. DHT, CT, TI and TIIA concentrations were measured using HPLC. NAA did not increase the concentration of any tanshinone. SA increased content in a concentration- and time-dependent manner; however, the yields were relatively low, possibly due to the metabolic specificity of S. miltiorrhiza cultivars in Poland. Total tanshinone concentration reached 226.38 ± 37.33 μg g−1 DW after 50 days of 0.4 mM SA elicitation. After 50 days of SA elicitation, the following maximum tanshinone concentrations were observed for 0.4 mM SA: DHT (71.58 ± 12.72 μg g−1 DW), CT (108.54 ± 18.29 μg g−1 DW), TI (29.50 ± 4.13 μg g−1 DW) and TIIA (16.75 ± 2.74 μg g−1 DW). To account for these observed differences in tanshinone biosynthesis, the distribution of SA and auxin responsive cis-active motif in the proximal promoters of the mevalonic acid, methylerythritol-4-phosphate and tanshinone-precursor biosynthesis pathway genes was evaluated in A. thaliana and S. miltiorrhiza. Our findings indicate that the SA-responsive cis-active elements have a much broader distribution than those recognized by auxin-responsive transcription factors.

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Section: Distribution Of Sa Responsive Cis-active Elements Within Promentioning

confidence: 96%

Elicitation of solid callus cultures of Salvia miltiorrhiza Bunge with salicylic acid and a synthetic auxin (1-naphthaleneacetic acid)

Szymczyk

Szymańska

Kochan

et al. 2021

Plant Cell Tiss Organ Cult

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“…In the present study, 24 TFs associated with tanshinones accumulation were obtained, including six NAC, four AP2/ERF, three WRKY, two MYB, and one MYB-related TFs. One ERF (Unigene0005368, ERF1B) and three WRKY (Unigene0002677, WRKY24; Unigene0005661, WRKY22; Unigene0019306, WRKY33) TFs related to the MAPK signaling pathway were identified, while one of them, WRKY33, was found to possibly regulate tanshinones accumulation (Jiang et al, 2019), suggesting that the possible regulatory role of the other three MAPK signaling pathway-related TFs as well as other tanshinones accumulation-related (Table S10). In addition, some members of the ERP, bHLH, and MYB transcription factor families have been previously identified to play important regulatory roles in tanshinone biosynthesis and accumulation.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analyses of differentSalvia miltiorrhizavarieties during the accumulation of tanshinones

Jingwen

Shuai

et al. 2021

PeerJ

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Salvia miltiorrhiza (Labiatae) is an important medicinal plant in traditional Chinese medicine. Tanshinones are one of the main active components of S. miltiorrhiza. It has been found that the intraspecific variation of S. miltiorrhiza is relatively large and the content of tanshinones in its roots of different varieties is also relatively different. To investigate the molecular mechanisms that responsible for the differences among these varieties, the tanshinones content was determined and comparative transcriptomics analysis was carried out during the tanshinones accumulation stage. A total of 52,216 unigenes were obtained from the transcriptome by RNA sequencing among which 23,369 genes were differentially expressed among different varieties, and 2,016 genes including 18 diterpenoid biosynthesis-related genes were differentially expressed during the tanshinones accumulation stage. Functional categorization of the differentially expressed genes (DEGs) among these varieties revealed that the pathway related to photosynthesis, oxidative phosphorylation, secondary metabolite biosynthesis, diterpenoid biosynthesis, terpenoid backbone biosynthesis, sesquiterpenoid and triterpenoid biosynthesis are the most differentially regulated processes in these varieties. The six tanshinone components in these varieties showed different dynamic changes in tanshinone accumulation stage. In addition, combined with the analysis of the dynamic changes, 277 DEGs (including one dehydrogenase, three CYP450 and 24 transcription factors belonging to 12 transcription factor families) related to the accumulation of tanshinones components were obtained. Furthermore, the KEGG pathway enrichment analysis of these 277 DEGs suggested that there might be an interconnection between the primary metabolic processes, signaling processes and the accumulation of tanshinones components. This study expands the vision of intraspecific variation and gene regulation mechanism of secondary metabolite biosynthesis pathways in medicinal plants from the “omics” perspective.

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“…It has been reported that the expression level of AACT is significantly correlated with tanshinone production [ 47 ]. SmDXS2 has been identified as a potential key enzyme in the pathway involved in targeted metabolic engineering to increase the accumulation of tanshinone in S. miltiorrhiza hairy roots [ 48 , 49 ]. CYP76AH1 has been demonstrated to catalyse the turnover of miltiradiene in tanshinone biosynthesis [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…Metabolic engineering can regulate secondary metabolism of S. miltiorrhiza through genetic modification of biosynthetic pathways [ 56 ]. Currently, most reports have focused on targeted metabolic engineering by key enzymes [ 8 , 49 , 57 ] or transcription factors [ 22 , 58 ]. Table 2 shows several genes (including SmGRAS3 , SmJAZ8 , and SmKSL ) and transcription factors (including SmWRKY2 , SmMYB36 , SmERF1L1 , SmERF115 , SmMYB98 , and SmWRKY1 ) that regulate the tanshinones biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Plasma Membrane H+-ATPase SmPHA4 Negatively Regulates the Biosynthesis of Tanshinones in Salvia miltiorrhiza

Zhang

et al. 2021

IJMS

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Salvia miltiorrhiza Bunge has been widely used in the treatment of cardiovascular and cerebrovascular diseases, due to the pharmacological action of its active components such as the tanshinones. Plasma membrane (PM) H+-ATPase plays key roles in numerous physiological processes in plants. However, little is known about the PM H+-ATPase gene family in S. miltiorrhiza (Sm). Here, nine PM H+-ATPase isoforms were identified and named SmPHA1–SmPHA9. Phylogenetic tree analysis showed that the genetic distance of SmPHAs was relatively far in the S. miltiorrhiza PM H+-ATPase family. Moreover, the transmembrane structures were rich in SmPHA protein. In addition, SmPHA4 was found to be highly expressed in roots and flowers. HPLC revealed that accumulation of dihydrotanshinone (DT), cryptotanshinone (CT), and tanshinone I (TI) was significantly reduced in the SmPHA4-OE lines but was increased in the SmPHA4-RNAi lines, ranging from 2.54 to 3.52, 3.77 to 6.33, and 0.35 to 0.74 mg/g, respectively, suggesting that SmPHA4 is a candidate regulator of tanshinone metabolites. Moreover, qRT-PCR confirmed that the expression of tanshinone biosynthetic-related key enzymes was also upregulated in the SmPHA4-RNAi lines. In summary, this study highlighted PM H+-ATPase function and provided new insights into regulatory candidate genes for modulating secondary metabolism biosynthesis in S. miltiorrhiza.

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Transcriptome sequencing of Salvia miltiorrhiza after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis

Cited by 26 publications

References 68 publications

Elicitation of solid callus cultures of Salvia miltiorrhiza Bunge with salicylic acid and a synthetic auxin (1-naphthaleneacetic acid)

Elicitation of solid callus cultures of Salvia miltiorrhiza Bunge with salicylic acid and a synthetic auxin (1-naphthaleneacetic acid)

Comparative transcriptome analyses of differentSalvia miltiorrhizavarieties during the accumulation of tanshinones

Plasma Membrane H+-ATPase SmPHA4 Negatively Regulates the Biosynthesis of Tanshinones in Salvia miltiorrhiza

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