Transcriptomic analysis of long noncoding RNAs and mRNAs expression profiles in the spinal cord of bone cancer pain rats

Hou, Xinran; Weng, Yingqi; Guo, Qulian; Ding, Zhuofeng; Wang, Jian; Dai, Jiajia; Wei, Anqi; Song, Zongbin

doi:10.1186/s13041-020-00589-2

Cited by 11 publications

(7 citation statements)

References 51 publications

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“…This review focuses on the latest research findings on the effect of lncRNAs on CIP and identifies the roles of lncRNAs in metastatic tumor-induced pain. Transcriptome sequencing and RT-qPCR validated the change in the expression of 10 lncRNAs (five upregulated and five downregulated) in the ipsilateral lumbar spinal cord in a rat BCP model ( Hou et al, 2020 ). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the dysregulated lncRNAs (NONRATT007487.2, NONRATT 003582.2, NONRATT026544.2, NONRATT004661.2, NONRATT008764.2, MSTRG.12616.2, MSTRG.13351.2, MSTRG.16194.3, MSTRG.16806.2, and MSTRG.29385.15) indicated that they were mainly involved in inflammatory and immunological responses.…”

Section: Lncrna and Chronic Cancer-related Painmentioning

confidence: 91%

“…This review focuses on the latest research findings on the effect of lncRNAs on CIP and identifies the roles of lncRNAs in metastatic tumor-induced pain. Transcriptome sequencing and RT-qPCR validated the change in the expression of 10 lncRNAs (five upregulated and five downregulated) in the ipsilateral lumbar spinal cord in a rat BCP model (Hou et al, 2020) MSTRG.16194.3, MSTRG.16806.2, and MSTRG.29385.15) indicated that they were mainly involved in inflammatory and immunological responses. Inflammation in the nervous system has been reported to play an important role in BCP, and inhibiting this response could significantly attenuate BCP (Song et al, 2015;Chen S.P.…”

Section: Lncrnas and Cancer-induced Painmentioning

confidence: 91%

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Roles of Long Non-coding RNAs in the Development of Chronic Pain

Jian

et al. 2021

Front. Mol. Neurosci.

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Chronic pain, a severe public health issue, affects the quality of life of patients and results in a major socioeconomic burden. Only limited drug treatments for chronic pain are available, and they have insufficient efficacy. Recent studies have found that the expression of long non-coding RNAs (lncRNAs) is dysregulated in various chronic pain models, including chronic neuropathic pain, chronic inflammatory pain, and chronic cancer-related pain. Studies have also explored the effect of these dysregulated lncRNAs on the activation of microRNAs, inflammatory cytokines, and so on. These mechanisms have been widely demonstrated to play a critical role in the development of chronic pain. The findings of these studies indicate the significant roles of dysregulated lncRNAs in chronic pain in the dorsal root ganglion and spinal cord, following peripheral or central nerve lesions. This review summarizes the mechanism underlying the abnormal expression of lncRNAs in the development of chronic pain induced by peripheral nerve injury, diabetic neuropathy, inflammatory response, trigeminal neuralgia, spinal cord injury, cancer metastasis, and other conditions. Understanding the effect of lncRNAs may provide a novel insight that targeting lncRNAs could be a potential candidate for therapeutic intervention in chronic pain.

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Section: Lncrna and Chronic Cancer-related Painmentioning

confidence: 91%

“…This review focuses on the latest research findings on the effect of lncRNAs on CIP and identifies the roles of lncRNAs in metastatic tumor-induced pain. Transcriptome sequencing and RT-qPCR validated the change in the expression of 10 lncRNAs (five upregulated and five downregulated) in the ipsilateral lumbar spinal cord in a rat BCP model (Hou et al, 2020) MSTRG.16194.3, MSTRG.16806.2, and MSTRG.29385.15) indicated that they were mainly involved in inflammatory and immunological responses. Inflammation in the nervous system has been reported to play an important role in BCP, and inhibiting this response could significantly attenuate BCP (Song et al, 2015;Chen S.P.…”

Section: Lncrnas and Cancer-induced Painmentioning

confidence: 91%

Roles of Long Non-coding RNAs in the Development of Chronic Pain

Jian

et al. 2021

Front. Mol. Neurosci.

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“…lncRNA X inactive specific transcript ( XIST ) results in the progression of neuropathic pain through regulating miR‐154‐5p and TLR5 in chronic constriction injury (CCI) rats (Wei et al, 2018). Transcriptome sequencing of the BCP rats provided a landscape of dysregulated lncRNAs in the spinal cord, indicating that lncRNAs may be potential targets for treatment in the future (Hou et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

LncRNA NONRATT009773.2 promotes bone cancer pain progression through the miR‐708‐5p/CXCL13 axis

Chen

Wang

et al. 2022

Eur J of Neuroscience

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Bone cancer pain (BCP) is the most frequently observed chronic cancer pain, and its development remains largely unexplored. Dysregulation of non‐coding RNAs greatly contributes to the pathogenesis of BCP. In the present study, we found a new long noncoding RNA (lncRNA), NONRATT009773.2, and investigated its role in the spinal cord of BCP rats. Our results showed that NONRATT009773.2 was significantly up‐regulated in BCP model rats, whereas depletion of NONRATT009773.2 attenuated BCP. In contrast, overexpression of NONRATT009773.2 triggered pain‐like symptoms in normal animals. Moreover, NONRATT009773.2 functioned as a microRNA (miRNA) sponge to absorb miR‐708‐5p and up‐regulated miRNA downstream target CXCL13, which plays fundamental roles in the initiation and maintenance of neuroinflammation and hyperalgesia. Collectively, our current findings indicated that NONRATT009773.2 could be employed as a new therapeutic target for BCP.

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“…Recently, more and more evidence has been accumulated to support the role of central nociceptive hypersensitivity in bone cancer pain [5,6].…”

Section: Introductionmentioning

confidence: 99%

MHCII Contributes to Bone Cancer Pain via Activating the NLRP3 Inflammasome in Microglia of the Medial Prefrontal Cortex

Wang

Zhang

et al. 2022

Preprint

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As one of the causes of bone cancer pain (BCP), central hyperalgesia has more and more supporting evidences recently. The medial prefrontal cortex (mPFC) is implicated in emotional disorders associated with BCP. Major histocompatibility complex II (MHCII) is a key component in regulating anti-inflammatory response and antigen presentation. This study aims to investigate whether MHCII of the mPFC participates in the BCP. Rat model of BCP was established by injecting Walker 256 cells into the tibia. BCP-induced decreased pain threshold, increased the expression of MHCII and the downstream protein CTSS, CD74 and upstream protein CIITA in mPFC, and the upregulation of MHCII in the mPFC of BCP rats was restricted in microglia. Furthermore, pharmacological inhibition of MHCII by intraperitoneal injecting minocycline provides relief from BCP. Importantly, the upregulation of MHCII is accompanied by significant NLRP3 inflammasome activated and inflammatory factors (IL-1β, IL-18 and TNF-α) released in the mPFC of BCP rats. Taken together, our data suggest that MHCII participates in BCP by activating inflammasome in microglia of the mPFC.

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Transcriptomic analysis of long noncoding RNAs and mRNAs expression profiles in the spinal cord of bone cancer pain rats

Cited by 11 publications

References 51 publications

Roles of Long Non-coding RNAs in the Development of Chronic Pain

Roles of Long Non-coding RNAs in the Development of Chronic Pain

LncRNA NONRATT009773.2 promotes bone cancer pain progression through the miR‐708‐5p/CXCL13 axis

MHCII Contributes to Bone Cancer Pain via Activating the NLRP3 Inflammasome in Microglia of the Medial Prefrontal Cortex

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