Transcriptomic analysis of Neocaridina denticulate sinensis hepatopancreas indicates immune changes after copper exposure

Xing, Kefan; Liu, Yujie; Yan, Congcong; Zhou, Yongzhao; Sun, Yuying; Zhang, Jiquan

doi:10.1016/j.fsi.2021.10.029

Cited by 15 publications

(6 citation statements)

References 30 publications

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“…Hemocyanin (HC), a precursor to AMPs, not only serves as an oxygen carrier but also plays a role in immune defense [54][55][56]. The expression level of HC was up-regulated in crustaceans following heavy metal exposure, thereby facilitating an immunological response [10,42]. In our study, HC (CL2.Contig14_All), domeless (CL5288.Contig1_All), stat (CL607.Contig1_All), and relish (CL2513.Contig3_All) were significantly induced after Cu exposure, indicating that the up-regulation of these genes might be a critical response in the resistance of M. rosenbergii to Cu toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…Accumulation of Cu in the organs of crustaceans leads to the overproduction of ROS, resulting in oxidative damage [10]. NADPH oxidase (NOX) is the main source of ROS in living organisms [65,66].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Cu can cause damage to the mitochondrial membrane of crustaceans and disrupt normal metabolism [6]. In recent years, Cu has been widely used in industrial production activities as an important conductive material and raw material for manufacturing alloys, as an effective algaecide for inhibiting toxic algal blooms, and as an essential mineral in feed [7][8][9][10]. These activities undoubtedly cause Cu contamination in aquaculture environments.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptome Analysis of the Hepatopancreas from Macrobrachium rosenbergii Exposed to the Heavy Metal Copper

Zhang,

Bao,

Guo

et al. 2024

Animals

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The contamination of aquatic ecosystems by the heavy metal copper (Cu) is an important environmental issue and poses significant risks to the physiological functions of aquatic organisms. Macrobrachium rosenbergii is one of the most important freshwater-cultured prawns in the world. The hepatopancreas of crustaceans is a key organ for immune defense, heavy metal accumulation, and detoxification, playing a pivotal role in toxicological research. However, research on the molecular response of the hepatopancreas in M. rosenbergii to Cu exposure is still lacking. In this study, the transcriptomic response in the hepatopancreas of M. rosenbergii was studied after Cu exposure for 3 and 48 h. Compared with the control group, 11,164 (7288 up-regulated and 3876 down-regulated genes) and 10,937 (6630 up-regulated and 4307 down-regulated genes) differentially expressed genes (DEGs) were identified after 3 and 48 h exposure, respectively. Most of these DEGs were up-regulated, implying that gene expressions were largely induced by Cu. Functional enrichment analysis of these DEGs revealed that immunity, copper homeostasis, detoxification, DNA damage repair, and apoptosis were differentially regulated by Cu. Seven genes involved in immunity, detoxification, and metabolism were selected for validation by qRT-PCR, and the results confirmed the reliability of RNA-Seq. All these findings suggest that M. rosenbergii attempts to resist the toxicity of Cu by up-regulating the expression of genes related to immunity, metabolism, and detoxification. However, with the excessive accumulation of reactive oxygen species (ROS), the antioxidant enzyme system was destroyed. As a result, DNA damage repair and the cellular stress response were inhibited, thereby exacerbating cell damage. In order to maintain the normal function of the hepatopancreas, M. rosenbergii removes damaged cells by activating the apoptosis mechanism. Our study not only facilitates an understanding of the molecular response mechanisms of M. rosenbergii underlying Cu toxicity effects but also helps us to identify potential biomarkers associated with the stress response in other crustaceans.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Transcriptome Analysis of the Hepatopancreas from Macrobrachium rosenbergii Exposed to the Heavy Metal Copper

Zhang,

Bao,

Guo

et al. 2024

Animals

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“…The freshwater shrimp Neocaridina davidi has not been utilized to filter parasites from water until now; however, it would appear to be a suitable and promising organism for testing the toxicity of chemical pollutants in the environment. It reacts sensitively to chemicals in the aquatic environment, has acute environmental toxicity, and is also used for testing the toxicity of pesticides and insecticides or testing microplastic retention ability [19,20,31].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Chemical and Biological Methods of Filtering Cryptosporidia from Water

Sučik

Valenčáková

2022

IJERPH

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Despite the fact that Cryptosporidium spp. is a parasite which commonly causes diarrhea, it still receives little attention. In our experiment, we focused on comparing the biological (N. davidi shrimp) and physical (zeolite with different thicknesses) possibility of filtering cryptosporidia from a small volume of water, which could contribute to increasing the catchability of this parasite. We monitored the ability to capture oocysts of the parasite Cryptosporidium parvum, genotype IIaA11G2R1, found in water samples. We infected drinking water with feces with a known number of cryptosporidial oocysts. One gram of sample contained ±28 oocysts. We filtered eight water samples with different concentrations of oocysts (0.1–2 g of infected stool per 15 L of water) using zeolite with a particle thickness of 0.2–0.6 mm and 0–0.3 mm. This was followed by purification, centrifugation and isolation utilizing the isolation kit AmpliSens® DNA-sorb-B, which is intended for stool. In total, 120 shrimp were divided into four aquariums (A, B, C, n = 30) including the control (K), while drinking water with the same parameters was infected with different concentrations of oocysts (A: 2.5 g, B: 2 g, C: 1 g of infected stool per 15 L of water). We took 10 individual shrimp and processed them in three time intervals (6 h, 12 h and 24 h). We processed them whole, and we isolated the DNA utilizing the isolation kit AmpliSens® DNA-sorb-AM, which is intended for tissues. Detection was carried out by molecular methods, namely the Nested PCR targeting of the region of the GP60 gene (60 kD glycoprotein). Gel electrophoresis showed the presence of C. parvum in seven zeolite-filtered water samples, and the parasite was not found in the water sample with the lowest number of oocysts filtered through the smaller-particle zeolite. There were 67 C. parvum-positive shrimp. Whereas the most positive shrimp were identified at 12 h of sampling, the least were identified at the 24 h mark. No shrimp positive for C. parvum was found in the control group. By sequencing, we confirmed the presence of C. parvum, genotype IIaA11G2R1, in all positive samples. We thus proved that the filtration capabilities of zeolite and N. davidi can be used for the rapid diagnosis of the presence of protozoa in a small amount of studied water.

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“…showed mortality in a range from 9.36 (8-10.96) µg/L to 454 (418-494) mg/L [59-61] (Table 3). In terms of sub-individual levels of N. denticulata, nonylphenol, dipropyl phthalate (DPrP), imidacloprid (IMI), lindane, chlordane, acetaminophen, Cu 2+ brought about the significant gene expression changes (immune defense, translation, metabolism, ribosomal gene expression, respiration, stress response, molting), endocrine disruption (estradiol, testosterone concentrations changes, incline of vitellogenin-like protein), oxidative stress (SOD and phenoloxidase activity), organ/tissue level changes (reduced heartbeat rate, gill ventilation) with locomotive activity changes [27,[62][63][64][66][67][68][69][70].…”

Section: Toxicity Study Statusmentioning

confidence: 99%

Proposing Effective Ecotoxicity Test Species for Chemical Safety Assessment in East Asia: A Review

Lee,

Shim,

Park

2023

Toxics

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East Asia leads the global chemical industry, but environmental chemical risk in these countries is an emerging concern. Despite this, only a few native species that are representative of East Asian environments are listed as test species in international guidelines compared with those native to Europe and America. This review suggests that Zacco platypus, Misgurnus anguillicaudatus, Hydrilla verticillata, Neocaridina denticulata spp., and Scenedesmus obliquus, all resident to East Asia, are promising test species for ecotoxicity tests. The utility of these five species in environmental risk assessment (ERA) varies depending on their individual traits and the state of ecotoxicity research, indicating a need for different applications of each species according to ERA objectives. Furthermore, the traits of these five species can complement each other when assessing chemical effects under diverse exposure scenarios, suggesting they can form a versatile battery for ERA. This review also analyzes recent trends in ecotoxicity studies and proposes emerging research issues, such as the application of alternative test methods, comparative studies using model species, the identification of specific markers for test species, and performance of toxicity tests under environmentally relevant conditions. The information provided on the utility of the five species and alternative issues in toxicity tests could assist in selecting test species suited to study objectives for more effective ERA.

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Transcriptomic analysis of Neocaridina denticulate sinensis hepatopancreas indicates immune changes after copper exposure

Cited by 15 publications

References 30 publications

Comparative Transcriptome Analysis of the Hepatopancreas from Macrobrachium rosenbergii Exposed to the Heavy Metal Copper

Comparative Transcriptome Analysis of the Hepatopancreas from Macrobrachium rosenbergii Exposed to the Heavy Metal Copper

Comparison of Chemical and Biological Methods of Filtering Cryptosporidia from Water

Proposing Effective Ecotoxicity Test Species for Chemical Safety Assessment in East Asia: A Review

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