Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp. cubense tropical race 4

Abstract

Fusarium wilt disease, caused by Fusarium oxysporum f. sp. cubense , especially by tropical race 4 ( Foc TR4), is threatening the global banana industry. Musa acuminata Pahang, a wild diploid banana that displays strong resistance to Foc TR4, holds great potential to understand the underlying resistance mechanisms. Microscopic examination reports that, in a wounding inoculation system, the Foc TR4 infection processes in roots of Pahang (resistant) and a triploid cultivar Brazilian (susceptible) were similar by 7 days post inoculation (dpi), but significant differences were observed in corms of both genotypes at 14 dpi. We compare transcriptomic responses in the corms of Pahang and Brazilian, and show that Pahang exhibited constitutive defense responses before Foc TR4 infection and inducible defense responses prior to Brazilian at the initial Foc TR4 infection stage. Most key enzymatic genes in the phenylalanine metabolism pathway were up-regulated in Brazilian, suggesting that lignin and phytotoxin may be triggered during later stages of Foc TR4 infection. This study unravels a few potential resistance candidate genes whose expression patterns were assessed by RT-qPCR assay and improves our understanding the defense mechanisms of Pahang response to Foc TR4.

Proteomic analysis of banana xylem sap provides insight into resistant mechanisms to Fusarium oxysporum f. sp. cubense Tropical Race 4

“…In the study, a GLIP (XP_009382515.1), LOC103970461 (Ma11_t05100), was increased abundance in TR4 inoculated Pahang compared with that of Brazilian, but no changes in other pairs. It further validated our previous transcriptomic study that one GLIP gene was activated by TR4 attack [59].…”

Combined De Novo Transcriptome and Metabolome Analysis of Common Bean Response to Fusarium oxysporum f. sp. phaseoli Infection

“…The present study reports the first combined de novo metabolome and RNA-seq analysis designed to describe the response of common bean infected with FOP. Previously, plant-FOP interaction has been demonstrated in many experiments in different crop plants [6][7][8][9][10][11][12][13][14][15][16]. In common bean it is known that colonization of FOP induces the defense responses in both a constitutive and inducible way.…”

“…Infected plants display stunting, complete wilting, extensive chlorosis, and necrosis on the leaves [4,5]. 2 of 17 Host-F. oxysporum pathosystems have been characterized in limited crops i.e., banana (Musa paradisiaca) [6,7], melon (Cucumis melo) [8,9], chickpea (Cicer arietinum) [10][11][12], cotton (Gossypium hirsutum) [13], tomato (Lycopersicon esculentum) [14], Arabidopsis [15,16], Medicago truncatula [17]. For each plant species, the respective Fusarium pathogens and a variety of defense mechanisms have been observed, including wound responses and hypersensitive reactions as well as many gene expression and metabolic changes.…”

Comparison transcriptome analysis revealed resistance differences of Cavendish bananas to Fusarium oxysporum f.sp. cubense race1 and race4

“…The unique interaction of Foc with banana provides pivotal molecular information for the breeding of resistant banana varieties. Previous studies reported the gene expression profiles for banana-Foc interactions [23] and compared the transcriptomes of Cavendish resistant and susceptible cultivars [16,18,19,21,22]. Nevertheless, scarce data exist for assessing the differences in the defenses of Cavendish banana varieties, such as 'Brazilian', against Foc1 and Foc4.…”

“…Similarly, Sun et al [21] studied the comparative transcriptome of Foc4 infected with the resistant variety 'Guijiao 9' and the susceptible variety 'Brazilian'. In addition, Zhang et al [22] subjected the rhizome samples of the resistant variety 'Pahang' and the susceptible variety 'Williams' to transcriptome analysis after Foc4 infection. The above studies were based on the comparative transcriptome analysis of susceptible varieties infected with Foc4 alone or simultaneously infected with disease-resistant and susceptible varieties.…”