Transcriptomic analysis reveals biosynthesis genes and transcription factors related to leaf anthocyanin biosynthesis in Aglaonema commutatum

Abstract

Background Aglaonema commutatum ‘Red Valentine’, as a foliage ornamental plant, is widely used for interior and exterior decoration because of its easy cultivation and management. However, reduced proportion of red foliage during large-scale production of A. commutatum seedlings is a frequent occurrence, which has considerable implications on the plant’s ornamental and market value. However, the molecular mechanisms underlying this phenomenon remain unclear. Results To explore the molecular basis of the variation in leaf color of A. commutatum Red Valentine, we performed transcriptome sequencing with the Illumina platform using two different varieties of A. commutatum, namely Red Valentine and a green mutant, at three different stages of leaf development. We annotated 63,621 unigenes and 14,186 differentially expressed genes by pairwise comparison. Furthermore, we identified 26 anthocyanin biosynthesis structural genes. The transcript per million (TPM) values were significantly higher for Red Valentine than for the green mutant in all three developmental stages, consistent with the high anthocyanin content of Red Valentine leaves. We detected positive transcription factors that may be involved in the regulation of anthocyanin biosynthesis using BLAST and through correlation analysis. Downregulation of these transcription factors may downregulate the expression of anthocyanin genes. We obtained full-length cDNA of the anthocyanin biosynthesis and regulatory genes and constructed phylogenetic trees to ensure accuracy of the analysis. Conclusions Our study provides insights into the molecular mechanisms underlying leaf variation in A. commutatum Red Valentine and may be used to facilitate the breeding of ornamental cultivars with high anthocyanin levels.

CsMYB67 participates in the flavonoid biosynthesis of summer tea leaves

“…The phylogenetic analysis showed CsTTG1 has high similarity to VcTTG1 and AcTTG1 [ 28 ]. VcTTG1 and AcTTG1 are, respectively, related with the anthocyanin biosynthesis in blueberry [ 29 ], and Aglaonema commutatum [ 30 ]. Fig.…”

Identification of HpMYB1 inducing anthocyanin accumulation in Hippeastrum Hybridum tepals by RNA-seq

“…However, the lack of reference genome data and high heterozygosity have greatly limited the research on the molecular mechanisms of key trait formation in this genus. Transcriptome sequencing provides a low-cost, rapid, and reliable method to monitor gene expression patterns and identify key genes at various developmental stages or under different physiological conditions in non-model plants lacking genomic information [ 35 , 36 ]. In grape hyacinth, transcriptome sequencing of the blue and white variants provided a foundation for future functional and molecular biological studies on flower color [ 37 ].…”

Functional Validation and Promoter DNA Methylation Analysis of the OfPAO Gene of Osmanthus fragrans ‘Yinbi Shuanghui’

“…Chlorophyll is a key component involved in photosynthesis, as it absorbs light energy and determines the color of plant leaves; at the same time, it is precisely because of its absorbance properties that it has potential intracellular phototoxicity. When plants are stimulated via external conditions, they can directly or indirectly affect the metabolic pathway of chlorophyll, changing the chlorophyll content present in the plant body, ultimately leading to leaf color mutations [26][27][28][29]. Thus, the synthesis and degradation of chlorophyll are tightly regulated during plant development [30,31].…”