Transcriptomic analysis reveals potential genes involved in tanshinone biosynthesis in Salvia miltiorrhiza

Chang, Yuan‐Jen; Wang, Meizhen; Li, Jiang; Lu, Shanfa

doi:10.1038/s41598-019-51535-9

Cited by 33 publications

(14 citation statements)

References 41 publications

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“…Tanshinones are mainly synthesised by the mevalonic acid (MVA) and methylerythritol 4‐phosphate (MEP) pathways 23,24 . Pi deficiency increased the accumulation of tanshinones by promoting the expression levels of key enzyme genes in the biosynthetic pathways, and the genes involved in secondary metabolism were TAT, RAS, DXS1, and others 25 .…”

Section: Resultsmentioning

confidence: 99%

The quality of wild Salvia miltiorrhiza from Dao Di area in China and its correlation with soil parameters and climate factors

Huang

Kong

Chen³

et al. 2020

Phytochemical Analysis

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Introduction Salvia miltiorrhiza is a frequently used herb in traditional Chinese medicine, and tanshinone IIA (Tan IIA) and salvianolic acid B (Sal Acid B) are two major extracts obtained from its dried root. The quality of herbal ingredients can be affected by environmental factors. Objective To evaluate the quality of wild S. miltiorrhiza and investigate the influence of soil constituents and parameters as well as climatic conditions and factors on the content of Tan IIA and Sal Acid B. Methodology We collected samples in 12 natural locations in the Dao Di area in China, the area in which S. miltiorrhiza grows, that results in a distinctive higher quality of medicinal materials from the harvested plant. The concentrations of Tan IIA and Sal Acid B were measured by high‐performance liquid chromatography (HPLC). Soil total carbon, total nitrogen, available nitrogen, available phosphorus, available potassium, and particle size distribution were determined. We also collected climate data using ArcGIS from the WorldClim database, and correlation tests, redundancy, and regression analyses were conducted to analyse the relationship and cluster the samples according to their chemical profile. Results The content of Tan IIA and Sal Acid B in most of the samples was significantly different (P < 0.05). Soil available phosphorus was considered as a key factor that influenced the quality of wild S. miltiorrhiza, and we found a significant negative association between the concentration of Tan IIA in roots and soil available phosphorus. Moreover, the accumulation of Tan IIA in S. miltiorrhiza was also significantly associated with precipitation in April, May, and October, maximum temperature in January, and standard deviation of temperature seasonality. There was no significant correlation between the content of Sal Acid B and ecological factors. In addition, samples collected from Mengshan, Hexian, and Lushi locations were rich in Tan IIA and tended to cluster together, whereas samples collected from Longquan and Huoshan locations tended to cluster and were poor in Tan IIA. Conclusion The Tan IIA content in samples collected from southern Anhui was significantly lower than that in other Dao Di locations. The content of Tan IIA was related more to the soil than the temperature. Compared with Tan IIA, Sal Acid B was less influenced by soil and climate factors. The findings of this study may provide helpful references for quality control of medicinal plants that exert pharmacological effects in humans.

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Section: Resultsmentioning

confidence: 99%

The quality of wild Salvia miltiorrhiza from Dao Di area in China and its correlation with soil parameters and climate factors

Huang

Kong

Chen³

et al. 2020

Phytochemical Analysis

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“…Moreover, the conversion of miltiradiene to tanshinone requires multi-oxidation reactions by CYPs (Mizutani and Sato, 2011 ; Xu et al, 2015 ). Through transcriptome data, four potential CYP450s may involve in tanshinone biosynthesis compared with the methyl jasmonate induction group (Chang et al, 2019 ). However, it requires a lot of hard work to identify and elucidate the functions of CYPs in tanshinone biosynthesis.…”

Section: Application Of Synthetic Biology To Produce Natural Drugs From Plantsmentioning

confidence: 99%

Recent Advances in the Synthetic Biology of Natural Drugs

Lei

et al. 2021

Front. Bioeng. Biotechnol.

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Natural drugs have been transformed and optimized during the long process of evolution. These compounds play a very important role in the protection of human health and treatment of human diseases. Sustainable approaches to the generation of raw materials for pharmaceutical products have been extensively investigated in drug research and development because chemical synthesis is costly and generates pollution. The present review provides an overview of the recent advances in the synthetic biology of natural drugs. Particular attention is paid to the investigations of drugs that may be mass-produced by the pharmaceutical industry after optimization of the corresponding synthetic systems. The present review describes the reconstruction and optimization of biosynthetic pathways for nine drugs, including seven drugs from plant sources and two drugs from microbial sources, suggesting a new strategy for the large-scale preparation of some rare natural plant metabolites and highly bioactive microbial compounds. Some of the suggested synthetic methods remain in a preliminary exploration stage; however, a number of these methods demonstrated considerable application potential. The authors also discuss the advantages and disadvantages of the application of synthetic biology and various expression systems for heterologous expression of natural drugs. Thus, the present review provides a useful perspective for researchers attempting to use synthetic biology to produce natural drugs.

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“…Therefore, RNA-Seq is a powerful tool for the capturing of both coding and non-coding sequences, the mining functional genes and the quantification of gene expression levels ( Ozsolak & Milos, 2011 ; Wang, Gerstein & Snyder, 2009 ; Zhang, He & Cai, 2018 ). At present, this approach was successfully used for transcriptome sequencing of some other medicinal plants, such as Zanthoxylum planispinum ( Kim et al, 2019 ), Trachyspermum ammi ( Soltani Howyzeh et al, 2018 ), Picrorhiza kurroa ( Vashisht et al, 2016 ), and Salvia miltiorrhiza ( Chang et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis of roots, stems, and leaves of Pueraria lobata (Willd.) Ohwi: identification of genes involved in isoflavonoid biosynthesis

Wang

Cui

2021

PeerJ

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Background Pueraria lobata (Willd.) Ohwi is a valuable herb used in traditional Chinese medicine. Isoflavonoids are the major bioactive compounds in P. lobata, namely puerarin, daidzin, glycitin, genistin, daidzein, and glycitein, which have pharmacological properties of anti-cardiovascular, anti-hypertension, anti-inflammatory, and anti-arrhythmic. Methods To characterize the corresponding genes of the compounds in the isoflavonoid pathway, RNA sequencing (RNA-Seq) analyses of roots, stems, and leaves of P. lobata were carried out on the BGISEQ-500 sequencing platform. Results We identified 140,905 unigenes in total, of which 109,687 were annotated in public databases, after assembling the transcripts from all three tissues. Multiple genes encoding key enzymes, such as IF7GT and transcription factors, associated with isoflavonoid biosynthesis were identified and then further analyzed. Quantitative real-time PCR (qRT-PCR) results of some genes encoding key enzymes were consistent with our RNA-Seq analysis. Differentially expressed genes (DEGs) were determined by analyzing the expression profiles of roots compared with other tissues (leaves and stems). This analysis revealed numerous DEGs that were either uniquely expressed or up-regulated in the roots. Finally, quantitative analyses of isoflavonoid metabolites occurring in the three P. lobata tissue types were done via high-performance liquid-chromatography and tandem mass spectrometry methodology (HPLC-MS/MS). Our comprehensive transcriptome investigation substantially expands the genomic resources of P. lobata and provides valuable knowledge on both gene expression regulation and promising candidate genes that are involved in plant isoflavonoid pathways.

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Transcriptomic analysis reveals potential genes involved in tanshinone biosynthesis in Salvia miltiorrhiza

Cited by 33 publications

References 41 publications

The quality of wild Salvia miltiorrhiza from Dao Di area in China and its correlation with soil parameters and climate factors

The quality of wild Salvia miltiorrhiza from Dao Di area in China and its correlation with soil parameters and climate factors

Recent Advances in the Synthetic Biology of Natural Drugs

Comparative transcriptome analysis of roots, stems, and leaves of Pueraria lobata (Willd.) Ohwi: identification of genes involved in isoflavonoid biosynthesis

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