Transcriptomic changes in sweetpotato peroxidases in response to infection with the root-knot nematode Meloidogyne incognita

Sung, Yeon Woo; Lee, Il Hwan; Shim, Donghwan; Lee, Kang-Lok; Nam, Ki Jung; Yang, Jung‐Wook; Lee, Jeung Joo; Kwak, Sang‐Soo; Kim, Yun-Hee

doi:10.1007/s11033-019-04911-7

Cited by 19 publications

(8 citation statements)

References 41 publications

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“…During the induced defense response, different resistance related genes can function in different ways in the contrasting sweetpotato cultivars. When examining gene expression data, the majority of the known resistance genes, such as those encoding ethylene biosynthesis-related ACC oxidases (G2553| TU4254, G3204| TU5260, and G20891| TU34171), polyprenol reductase (G18703| TU30556), trehalose 6-phosphate phosphatases (G42399| TU69169 and G42693| TU69581) and cationic peroxidases (G41119| TU67300 and G45573| TU73531), which are upregulated, especially in SCs, upon nematode infection, are often the central focus, as pathogen resistance pathways are expected to be induced ( Figure 4 and Supplementary Table 3 ; Bajda et al, 2009 ; Melillo et al, 2014 ; Zhang et al, 2016 ; Leonetti et al, 2017 ; Sung et al, 2019 ). There can also be considerable value in genes downregulated upon RKN infection, especially in SCs, which may explain RKN success ( Figure 4 and Supplementary Table 4 ) as noted in other studies ( Guimaraes et al, 2015 ; Shukla et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…We previously conducted proteome and transcriptome profiling of two sweetpotato cultivars, Yulmi and Juhwangmi, with contrasting responses to infection with M. incognita ( Ha et al, 2017 ; Lee et al, 2019 ). Recently, we identified and studied the responses of candidate genes to RKN infection in these two cultivars ( Sung et al, 2019 ; Lee et al, 2020 ). However, these studies were limited in their ability to elucidate the mechanism of RKN resistance in sweetpotato because the analysis was limited to only two cultivars, and the late response to RKN (generation of number of galls) was confirmed after 2 months in the growth chamber and 3 months in the greenhouse.…”

Section: Introductionmentioning

confidence: 99%

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The Defense Response Involved in Sweetpotato Resistance to Root-Knot Nematode Meloidogyne incognita: Comparison of Root Transcriptomes of Resistant and Susceptible Sweetpotato Cultivars With Respect to Induced and Constitutive Defense Responses

et al. 2021

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Sweetpotato (Ipomoea batatas [L.] Lam) is an economically important, nutrient- and pigment-rich root vegetable used as both food and feed. Root-knot nematode (RKN), Meloidogyne incognita, causes major yield losses in sweetpotato and other crops worldwide. The identification of genes and mechanisms responsible for resistance to RKN will facilitate the development of RKN resistant cultivars not only in sweetpotato but also in other crops. In this study, we performed RNA-seq analysis of RKN resistant cultivars (RCs; Danjami, Pungwonmi and Juhwangmi) and susceptible cultivars (SCs; Dahomi, Shinhwangmi and Yulmi) of sweetpotato infected with M. incognita to examine the induced and constitutive defense response-related transcriptional changes. During induced defense, genes related to defense and secondary metabolites were induced in SCs, whereas those related to receptor protein kinase signaling and protein phosphorylation were induced in RCs. In the uninfected control, genes involved in proteolysis and biotic stimuli showed differential expression levels between RCs and SCs during constitutive defense. Additionally, genes related to redox regulation, lipid and cell wall metabolism, protease inhibitor and proteases were putatively identified as RKN defense-related genes. The root transcriptome of SCs was also analyzed under uninfected conditions, and several potential candidate genes were identified. Overall, our data provide key insights into the transcriptional changes in sweetpotato genes that occur during induced and constitutive defense responses against RKN infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Defense Response Involved in Sweetpotato Resistance to Root-Knot Nematode Meloidogyne incognita: Comparison of Root Transcriptomes of Resistant and Susceptible Sweetpotato Cultivars With Respect to Induced and Constitutive Defense Responses

et al. 2021

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“…Moreover, enhanced activities of peroxidases (APOX, GPOX and POD) are directly linked to their involvement in providing structural rigidity to plant tissues through enhanced lignin synthesis in cell walls in order to prevent the nematode penetration in plants [83]. Increases in the activities of peroxidases have also been reported in Ipomoea batatas subjected to PPN infection as assessed through transcriptomic analysis [84]. Moreover, an elevation in GPOX, CAT and SOD activities was observed in tomato plants infected by M. incognita [85].…”

Section: Discussionmentioning

confidence: 99%

Impact of Plant Growth Promoting Rhizobacteria in the Orchestration of Lycopersicon esculentum Mill. Resistance to Plant Parasitic Nematodes: A Metabolomic Approach to Evaluate Defense Responses Under Field Conditions

et al. 2019

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The present study deals with biological control of Meloidogyne incognita in 45-days old Lycopersicon esculentum, inoculated with Pseudomonas aeruginosa(M1) and Burkholderia gladioli (M2). The improved plant growth and biomass of nematode infested Plant growth promoting rhizobacteria (PGPR) inoculated plants was observed. Remarkable reduction in the numbers of second stage juvenile (J2s), root galls was recorded after treatment of microbes relative to experimental controls. Moreover, the lowered activities of oxidative stress markers (H2O2 (hydrogen peroxide), O2− (superoxide anion), malondialdehyde (MDA)) was estimated in plants after rhizobacterial supplementation. Higher activities of enzymatic (SOD (Superoxide dismutase), POD (Guaiacol peroxidase), CAT (Catalase), GPOX (Glutathione peroxidase), APOX (Ascorbate peroxidase), GST (Glutathione-S-transferase), GR (Glutathione reductase), DHAR (Dehydroascorbate reductase), PPO (Polyphenol oxidase)) and non-enzymatic (glutathione, ascorbic acid, tocopherol) antioxidants were further determined in nematode infected plants following the addition of bacterial strains. The upregulation of photosynthetic activities were depicted by evaluating plant pigments and gas exchange attributes. An increase in the levels of phenolic compounds (total phenols, flavonoids, anthocyanins), osmoprotectants (total osmolytes, carbohydrates, reducing sugars, trehalose, proline, glycine betaine, free amino acids) and organic acids (fumaric, succinic, citric, malic acid) were reflected in infected plants, showing further enhancement after application of biocontrol agents. The study revealed the understanding of plant metabolism, along with the initiative to commercially exploit the biocontrol agents as an alternative to chemical nematicides in infected fields for sustainable agriculture.

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“…DAO facilitates IAA oxidation, but it is anticipated that additional inactivation mechanisms are involved. In this model, peroxidases remain regulated by phenols but now enhance ROS production as reported bySimonetti et al (2010);Jin et al (2011), andSung et al (2019). IAA, indole-3-acetic acid; IAAox, oxidized indole-3-acetic acid; DAO, DIOXYGENASE FOR AUXIN OXIDATION; ROS, reactive oxygen species.…”

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confidence: 71%

“…It was shown for M. incognita and G. rostochiensis that the ratio between mono-and poly-phenols correlates to susceptibility (Giebel, 1970;Yuan et al, 1996). A plausible function of peroxidases in mediating resistance (Simonetti et al, 2010;Jin et al, 2011;Sung et al, 2019) is the generation of reactive oxygen species (ROS). ROS stimulate cell wall reinforcement through lignification and extensin crosslinking and induce additional defence mechanisms (Almagro et al, 2009;Marhavý et al, 2019).…”

Section: Manipulation Of Auxin Catabolism In Nematode Feeding Sitesmentioning

confidence: 99%

Sedentary Plant-Parasitic Nematodes Alter Auxin Homeostasis via Multiple Strategies

et al. 2021

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Sedentary endoparasites such as cyst and root-knot nematodes infect many important food crops and are major agro-economical pests worldwide. These plant-parasitic nematodes exploit endogenous molecular and physiological pathways in the roots of their host to establish unique feeding structures. These structures function as highly active transfer cells and metabolic sinks and are essential for the parasites’ growth and reproduction. Plant hormones like indole-3-acetic acid (IAA) are a fundamental component in the formation of these feeding complexes. However, their underlying molecular and biochemical mechanisms are still elusive despite recent advances in the field. This review presents a comprehensive overview of known functions of various auxins in plant-parasitic nematode infection sites, based on a systematic analysis of current literature. We evaluate multiple aspects involved in auxin homeostasis in plants, including anabolism, catabolism, transport, and signalling. From these analyses, a picture emerges that plant-parasitic nematodes have evolved multiple strategies to manipulate auxin homeostasis to establish a successful parasitic relationship with their host. Additionally, there appears to be a potential role for auxins other than IAA in plant-parasitic nematode infections that might be of interest to be further elucidated.

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Transcriptomic changes in sweetpotato peroxidases in response to infection with the root-knot nematode Meloidogyne incognita

Cited by 19 publications

References 41 publications

The Defense Response Involved in Sweetpotato Resistance to Root-Knot Nematode Meloidogyne incognita: Comparison of Root Transcriptomes of Resistant and Susceptible Sweetpotato Cultivars With Respect to Induced and Constitutive Defense Responses

The Defense Response Involved in Sweetpotato Resistance to Root-Knot Nematode Meloidogyne incognita: Comparison of Root Transcriptomes of Resistant and Susceptible Sweetpotato Cultivars With Respect to Induced and Constitutive Defense Responses

Impact of Plant Growth Promoting Rhizobacteria in the Orchestration of Lycopersicon esculentum Mill. Resistance to Plant Parasitic Nematodes: A Metabolomic Approach to Evaluate Defense Responses Under Field Conditions

Sedentary Plant-Parasitic Nematodes Alter Auxin Homeostasis via Multiple Strategies

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