Transcriptomic data from the rat liver after five days of exposure to legacy or emerging brominated flame retardants

Abstract

Large-scale gene expression analysis of legacy* and emerging** brominated flame retardants were conducted in the male Harlan Sprague Dawley rat [1] . Each animal was dosed for 5 days with the chemical at concentrations of 0.1 – 1000 μmol/kg body weight per day. Following the last dose, a specimen of the left liver was removed for RNA extraction. The amplified RNA (aRNA) was fragmented and then hybridized to Affymetrix Rat Genome 230 2.0 Arrays. Each GeneChip® array was scanned using an Affymetrix GeneChip® Scanner 3000 7 G to generate raw expression level data (.CEL files). Statistical contrasts were used to find pairwise gene expression differences between the control group and each dose group using the R/maanova package [2] . The transcriptomic data can be used to provide insights into the degree of toxicity, toxic mechanisms, disease pathways activated by exposure, and for benchmark dose analysis. The gene expression data for each of the nine flame retardants discussed here accompanies the research article entitled, “Comparative Toxicity and Liver Transcriptomics of Legacy and Emerging Brominated Flame Retardants following 5-Day Exposure in the Rat” [1] . * polybrominated diphenyl ether 47 (PBDE 47), decabromodiphenyl ether (decaBDE), hexabromocyclododecane (HBCD); ** 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (TBB); bis(2-ethylhexyl) tetrabromophthalate (TBPH); tetrabromobisphenol A-bis(2,3-dibromopropyl ether (TBBPA-DBPE); 1,2-bis(tribromophenoxy)ethane (BTBPE); decabromodiphenylethane (DBDPE); hexachlorocyclopentadienyl-dibromocyclooctane (HCDBCO).

Tetrabromobisphenol A-bis(2,3-dibromopropyl ether) impairs Postnatal Testis Development in Mice: The Microtubule Cytoskeleton as a Sensitive Target

“…1 A transcriptome study also revealed little perturbation in the liver of adult rats following a 5-day exposure to TBBPA-BDBPE. 11 These data seem to be in agreement with several in vitro studies reporting that TBBPA-BDBPE could have limited potency to interact with typical nuclear receptors. 12,13 In contrast, Yao et al reported that a 7-day exposure to TBBPA-BDBPE at a low dose (57 nmol/kg body weight/d) significantly increased the triacylglycerol content in liver tissues and the number of hepatic macrophage in adult mice.…”

Gene expression profiling after exposure to a chemical carcinogen, Pentabrominated Diphenyl Ether, at different life stages

“…The animals used were either male Wistar Han rat or Harlan Sprague Dawley rats which induced transcripts encoding metabolic enzymes, members of the Nrf2 antioxidant pathway, and ABC transporters upon exposure to PBDE-47 and the PBDE-47 mixture as described in previous publications ( Dunnick et al, 2018c ; Shockley et al, 2020a ; Shockley et al, 2020b ; Dunnick et al, 2020 ). In this paper, the transcriptomic data from different life stages was analyzed by groups (Group 1–5, see Table 1 ).…”

“…Group One examined rat liver toxicogenomic changes after PBDE-47 exposure for 5 days. In this study adult Harlan Sprague Dawley male rats were exposed to the PBDE mixture by oral gavage in corn oil for five consecutive days; on the sixth day liver samples were taken for toxicogenomic analysis ( Shockley et al, 2020a ; Shockley et al, 2020b ). Group Two and Group Four examined rat liver toxicogenomic changes in PND 4 pups after exposure of dams to PBDE-47 or the PBDE mixture (DE-71), respectively ( Dunnick et al, 2020 ).…”

Update of the scientific opinion on tetrabromobisphenol A (TBBPA) and its derivatives in food

“…Associated with changes in number and distribution of lysosomes and calcium ino‐mediated lysosomal exocytosis Shockley et al. ( 2020b ). Transcriptomic data from the rat liver after 5 days of exposure to legacy or emerging brominated flame retardants This is accompanying data providing toxicogenomic data for others to use Guan et al.…”