Transcriptomic profiling of maize (Zea mays L.) seedlings in response to Pseudomonas putida stain FBKV2 inoculation under drought stress

SkZ, Ali; Vardharajula, Sandhya; Vurukonda, Sai Shiva Krishna Prasad

doi:10.1007/s13213-018-1341-3

Cited by 40 publications

(12 citation statements)

References 94 publications

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“…Plants inoculated (no matter which strain) showed a higher root colonization ability and biomass under water stress compared to the control. These results are in accordance with SkZ et al (2018), who found higher biomass of increased root colonization of P. putida ‐inoculated Zea mays L. seedlings. Ghosh et al (2018) also reported that P. putida GAP‐P45 can resist drought stress.…”

Section: Discussionsupporting

confidence: 93%

Drought‐tolerant Pseudomonas sp. showed differential expression of stress‐responsive genes and induced drought tolerance in Arabidopsis thaliana

Yasmin

Bano

Wilson

et al. 2021

Physiologia Plantarum

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The growth and persistence of rhizobacteria in soils are highly impacted by moisture stress. In this study, we report the first transcript analysis of four Pseudomonas strains (PS1, PS2, PS3, and PS4) isolated from the root‐soil interface of rice and maize associated with different moisture levels during water deprivation. Filtered Pseudomonas sp. cells incubated at low (RH10%) and high (RH85%) relative humidity showed decreased survival of all Pseudomonas sp. at RH10% when compared with RH85%. RT‐PCR showed differential expression of treS (trehalose synthase), rpoS (sigma factor), mucA (alginate regulatory gene), and fliM (flagellar motor switch protein gene) in response to exposure to RH10%. However, molecular fingerprinting and nutrient assimilation profile of Pseudomonas strains demonstrated genetic and physiological variation between the four strains irrespective of water regime and host. In vitro testing of these strains showed ACC deaminase activity and gibberellic acid, abscisic acid, indole acetic acid, and exopolysaccharide production. We determined that 50 μl of 1.2 × 103 CFU ml‐1 of these Pseudomonas strains was enough to protect Arabidopsis plants against drought stress in a pot experiment. Inoculated plants increased their root colonization ability and biomass; however, PS2 showed higher survival (95%), relative water content (59%), chlorophyll (30%), glycine betaine (38%), proline (23%), and reduced MDA (43%) in shoots than irrigated control under induced water deprivation. It can be concluded that all Pseudomonas strains were effective in mitigating drought stress, however, PS2 appears to impart more resistance to drought than the other strains by upregulating key defense mechanisms.

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Section: Discussionsupporting

confidence: 93%

Drought‐tolerant Pseudomonas sp. showed differential expression of stress‐responsive genes and induced drought tolerance in Arabidopsis thaliana

Yasmin

Bano

Wilson

et al. 2021

Physiologia Plantarum

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“…ABA protects plants from dehydration by stimulating expression of dehydrins (Shakirova et al, 2016). In accordance with this effect of ABA, genes involved in the synthesis of dehydrins [late embryogenesis abundant (LEA) proteins] were upregulated in maize plants inoculated with P. putida strain FBKV2 (SkZ et al, 2018). FBKV2 inoculation enhanced SnRK2 family proteins, which facilitated transcription of ABA-responsive genes, thereby conferring drought tolerance.…”

Section: Introductionmentioning

confidence: 90%

Phytohormone Mediation of Interactions Between Plants and Non-Symbiotic Growth Promoting Bacteria Under Edaphic Stresses

et al. 2019

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The capacity of rhizoshere bacteria to influence plant hormonal status, by bacterial production or metabolism of hormones, is considered an important mechanism by which they promote plant growth, and productivity. Nevertheless, inoculating these bacteria into the plant rhizosphere may produce beneficial or detrimental results depending on bacterial effects on hormone composition and quantity in planta, and the environmental conditions under which the plants are growing. This review considers some effects of bacterial hormone production or metabolism on root growth and development and shoot physiological processes. We analyze how these changes in root and shoot growth and function help plants adapt to their growth conditions, especially as these change from optimal to stressful. Consistent effects are addressed, along with plant responses to specific environmental stresses: drought, salinity, and soil contamination (with petroleum in particular).

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“…Secondly, VOCs exposed seedlings further augmented the ROS scavenging machinery and exhibited higher activity of SOD, POD, CAT, APX, PPO, and PAL and reduced the drought stress than non‐exposed control. The role of PGPR in the alleviation of drought stress by augmenting the ROS scavenging enzyme system is well documented in the literature (Azmat et al, 2020; Ilyas et al, 2020; Khan et al, 2020; SkZ et al, 2018; Yasmin et al, 2017). However, for the first‐time current study showed BVOCs‐mediated alleviation of drought stress by modulating ROS scavenging machinery.…”

Section: Discussionmentioning

confidence: 95%

Volatile organic compounds produced by Pseudomonas pseudoalcaligenes alleviated drought stress by modulating defense system in maize (Zea mays L.)

Yasmin

Rashid

Hassan

et al. 2021

Physiologia Plantarum

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Research on plant growth-promoting bacteria (PGPR) revealed an effective role of bacterial volatile organic compounds (VOCs) in stress alleviation. Out of 15 PGPR strains, infection with VOCs from Pseudomonas pseudoalcaligenes' resulted in maximum germination, growth promotion, and drought tolerance in maize plants. The VOCs of P. pseudoalcaligenes caused induced systemic tolerance in maize plants during 7 days of drought stress. The VOCs exposed plants displayed resistance to drought stress by reducing electrolyte leakage and malondialdehyde content and increasing the synthesis of photosynthetic pigments, proline, and phytohormones contents. Maize plants revealed enhanced resistance by showing higher activities of antioxidant defense enzymes both in shoots and roots under drought stress. Activities of antioxidant enzymes were more pronounced in shoots than roots. Gas chromatography and mass spectrophotometric (GC-MS) analysis comparing VOCs produced by the most efficient P. pseudoalcaligenes strain and inefficient strains of Pseudomonas sp. grown in culture media revealed nine compounds that they had in common. However, dimethyl disulfide, 2,3-butanediol, and 2-pentylfuran were detected only in P. pseudoalcaligenes, indicating these compounds are potential candidates for drought stress induction. Further studies are needed to unravel the molecular mechanisms of VOCs-mediated systemic drought tolerance in plants related to each identified VOC.

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Transcriptomic profiling of maize (Zea mays L.) seedlings in response to Pseudomonas putida stain FBKV2 inoculation under drought stress

Cited by 40 publications

References 94 publications

Drought‐tolerant Pseudomonas sp. showed differential expression of stress‐responsive genes and induced drought tolerance in Arabidopsis thaliana

Drought‐tolerant Pseudomonas sp. showed differential expression of stress‐responsive genes and induced drought tolerance in Arabidopsis thaliana

Phytohormone Mediation of Interactions Between Plants and Non-Symbiotic Growth Promoting Bacteria Under Edaphic Stresses

Volatile organic compounds produced by Pseudomonas pseudoalcaligenes alleviated drought stress by modulating defense system in maize (Zea mays L.)

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