Transcriptomic response of the harmful algae Heterosigma akashiwo to polyphosphate utilization and phosphate stress

Ji, Nanjing; Zhang, Zhenzhen; Chen, Lei; Xu, Mingyang; Yin, Xueyao; Shen, Xin

doi:10.1016/j.hal.2022.102267

Cited by 13 publications

(7 citation statements)

References 48 publications

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“…The annotation rate in this study is higher than that of the do novo transcriptome assembly of H . akashiswo (approximately 37%) but it is comparable to those of other bloom-forming algae [ 13 , 43 , 44 ]. These results also highlight the difficulty in annotating the transcriptome of non-model organisms [ 40 ].…”

Section: Discussionmentioning

confidence: 95%

“…akashiwo , including encystation formation and diel vertical migration, are probably related to its bloom formation [ 12 , 18 ]. To explore the molecular mechanisms of these features, in several studies, targeted approaches, such as gene cloning, quantitative real-time PCR, and Illumina sequencing have been used [ 13 , 14 , 24 , 39 ]. However, molecular studies on HAB species have evolved slowly.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, previous studies have shown that H . akashiwo can utilize different nitrogen (N) sources (nitrate, ammonium, and urea) and dissolved organic phosphorus (e.g., glucose-6-phosphate and adenosine 5-triphosphate) [ 11 , 13 , 14 , 15 ]. Recently, Healey et al (2023) proposed that utilization of nitric oxide (NO) may give H .…”

Section: Introductionmentioning

confidence: 99%

“…For instance, the transcriptional responses of H . akashiwo to nutrient stress (nitrate and phosphate) were studied using next-generation sequencing (NGS) [ 13 , 24 ]. Furthermore, NGS was also used to explore the gene expression of the response of H .…”

Section: Introductionmentioning

confidence: 99%

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Full-Length Transcriptome Analysis of the Ichthyotoxic Harmful Alga Heterosigma akashiwo (Raphidophyceae) Using Single-Molecule Real-Time Sequencing

Ji¹,

Yin²,

Chen³

et al. 2023

Microorganisms

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The raphidophyte Heterosigma akashiwo is a harmful algal species. The bloom of this organism has been associated with the massive mortality of fish in many coastal waters. To investigate the molecular mechanism of H. akashiwo blooms, having a reliable reference transcriptome of this species is essential. Therefore, in this study, a full-length transcriptome of H. akashiwo was obtained by single-molecule real-time sequencing. In total, 45.44 Gb subread bases were generated, and 16,668 unigenes were obtained after the sequencing data processing. A total of 8666 (52.00%) unigenes were successfully annotated using seven public databases. Among them, mostly phosphorus and nitrogen metabolism genes were detected. Moreover, there were 300 putative transcription factors, 4392 putative long non-coding RNAs, and 7851 simple sequence repeats predicted. This study provides a valuable reference transcriptome for understanding how H. akashiwo blooms at a molecular level.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Full-Length Transcriptome Analysis of the Ichthyotoxic Harmful Alga Heterosigma akashiwo (Raphidophyceae) Using Single-Molecule Real-Time Sequencing

Ji¹,

Yin²,

Chen³

et al. 2023

Microorganisms

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“…Core genes were defined as those showing both high and stable expression [27]. Here, genes with expression ≥ 90th percentile FPKM (Fragments Per Kilobase of mRNA sequence per Million mapped reads) and coefficients of variation (CV) ≤ 0.08 across six samples (three control, three treatment) were collected.…”

Section: Identification Of Core Genes and Verification Of Degsmentioning

confidence: 99%

Physiological and Transcriptomic Response of Asiatic Hard Clam Meretrix meretrix to the Harmful Alga Heterosigma akashiwo

Xu²,

Li³

et al. 2023

Fishes

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The ichthyotoxic raphidophyte Heterosigma akashiwo is associated with harmful algal blooms (HABs). Although the influence of H. akashiwo on fish has been reported, little is known of its effects on other marine organisms, such as shellfish. In this study, the physiological and transcriptomic responses of the Asiatic hard clam Meretrix meretrix to H. akashiwo were investigated. The results showed that clam survival was not influenced by H. akashiwo (at a concentration of 2 × 104 cells/mL), and M. meretrix eliminated the alga effectively after 48 h in co-culture. H. akashiwo did not alter the antioxidant capacity of the clams. However, 544 unigenes were found to be differentially expressed between the control and H. akashiwo-treated clams. Interestingly, the significant up-regulation of lysosome-related genes and transport proteins suggested remodeling of the clam metabolism possibly for digestion of H. akashiwo. In summary, our results indicate that the clam M. meretrix can effectively feed on H. akashiwo, and intensification of shellfish farming may be helpful to control and mitigate H. akashiwo blooms.

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In vivo quantification of polyphosphate in Microcystis aeruginosa using DAPI staining

Zhu,

Tan,

Duan

et al. 2025

Algal Research

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Transcriptomic response of the harmful algae Heterosigma akashiwo to polyphosphate utilization and phosphate stress

Cited by 13 publications

References 48 publications

Full-Length Transcriptome Analysis of the Ichthyotoxic Harmful Alga Heterosigma akashiwo (Raphidophyceae) Using Single-Molecule Real-Time Sequencing

Full-Length Transcriptome Analysis of the Ichthyotoxic Harmful Alga Heterosigma akashiwo (Raphidophyceae) Using Single-Molecule Real-Time Sequencing

Physiological and Transcriptomic Response of Asiatic Hard Clam Meretrix meretrix to the Harmful Alga Heterosigma akashiwo

In vivo quantification of polyphosphate in Microcystis aeruginosa using DAPI staining

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