Transcriptomic response of the insect vector, Peregrinus maidis, to Maize mosaic rhabdovirus and identification of conserved responses to propagative viruses in hopper vectors

Martin, Kathleen M.; Alviar, Karen B.; Schneweis, Derek J.; Stewart, Catherine Louise; Rotenberg, Dorith; Whitfield, Anna E.

doi:10.1016/j.virol.2017.05.019

Cited by 26 publications

(37 citation statements)

References 55 publications

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“…5 for bumblebee EVEs), suggesting that integrations might have occured due to non-homologous recombination between an RNA of the progenitor virus and that of a retrotransposon during reverse transcription of the retrotransposon (Ballinger et al, 2012;Geuking et al, 2009;Horie et al, 2010). Interestingly, the transcription of several transposable elements (i.e., putative RNA-directed DNA polymerases) was induced by a plant rhabdovirus or plant reovirus infection in their vector insects (plant/leafhoppers) (Martin et al, 2017). The current and previous studies (Lazareva et al, 2015) (Fig.…”

Section: Endogenization Of Virga/nege-like Viruses Into the Insect Gementioning

confidence: 64%

A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes

et al. 2019

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Insects are the host and vector of diverse viruses including those that infect vertebrates, plants, and fungi. Recent wide-scale transcriptomic analyses have uncovered the existence of a number of novel insect viruses belonging to an alphavirus-like superfamily (virgavirus/negevirus-related lineage). In this study, through an in silico search using publicly available insect transcriptomic data, we found numerous virus-like sequences related to insect virga/nege-like viruses. Phylogenetic analysis showed that these novel viruses and related virus-like sequences fill the major phylogenetic gaps between insect and plant virga/negevirus lineages. Interestingly, one of the phylogenetic clades represents a unique insect-infecting virus group. Its members encode putative coat proteins which contained a conserved domain similar to that usually found in the coat protein of plant viruses in the family Virgaviridae. Furthermore, we discovered endogenous viral elements (EVEs) related to virga/nege-like viruses in the insect genomes, which enhances our understanding on their evolution. Database searches using the sequence of one member from this group revealed the presence of EVEs in a wide range of insect species, suggesting that there has been prevalent infection by this virus group since ancient times. Besides, we present detailed EVE integration profiles of this virus group in some species of the Bombus genus of bee families. A large variation in EVE patterns among Bombus species suggested that while some integration events occurred after the species divergence, others occurred before it. Our analyses support the view that insect and plant virga/nege-related viruses might share common virus origin(s).

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Section: Endogenization Of Virga/nege-like Viruses Into the Insect Gementioning

confidence: 64%

A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes

et al. 2019

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“…Thus, the virus can achieve the proliferation and cellular fitness by altering the host transcriptome responses, such as cellular responses associated with antiviral immunity and cell death/apoptosis. This remodeling effect of the virus on the host transcriptome is a common strategy exploited by the virus in hostvirus interactions and has been observed in various viruses [48][49][50]. Notably, a recent study showed that HSV-1 infection could not only result in changes in gene expression but also in alternative polyadenylation and splicing in the host transcriptome by influencing the host RNA processing [51].…”

Section: Discussionmentioning

confidence: 85%

Deep RNA Sequencing Reveals a Repertoire of Human Fibroblast Circular RNAs Associated with Cellular Responses to Herpes Simplex Virus 1 Infection

Shi¹,

Hu²,

Mo³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Circular RNAs (circRNAs), a new class of regulators of gene expression, are involved in diverse physiological and pathogenic processes. However, their role in cellular responses to virus infection is yet unclear. Methods: A human lung fibroblast cell line was infected or mock infected by herpes simplex virus 1 (HSV-1). Deep RNA sequencing was used to profile the global changes in circRNAs, genes, and miRNAs following HSV-1 infection. Altered circRNAs, genes, and miRNAs were validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR). An integration analysis of circRNAs, genes, and miRNAs was applied to investigate the putative function of the dysregulated circRNAs. Results: A total of 536 circRNAs, 3,885 genes, and 207 miRNAs were significantly dysregulated after HSV-1 infection. An integration analysis of circRNAs, genes, and miRNAs revealed the alleged involvement of dysregulated circRNAs in cellular responses to HSV-1 infection via the circRNA-miRNA-gene regulatory axis. These genes regulated by circRNAs were enriched to NOD-like receptor/JAK-STAT signaling pathway, and pathways of apoptosis, cell cycle progression, and cell death, all of which may be implicated in the viral pathogenesis and cellular immunity. Conclusions: These data present a comprehensive view for circRNAs induced by HSV-1 and their interplay with miRNAs and genes during HSV-1 infection, thus offering new insights into the mechanisms of interactions between HSV-1 and the host.

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“…In plants, the Barley yellow dwarf relies on the action of EIF4 for translation [53], as well as the human viruses Coxsackievirus [54] and Foot and Mouth virus [55]. Within insects EIF4 has been associated only with RNA Rhabdovirus and then in the negative sense of RNA Rhabdovirus [56]. Interestingly the role of EIF4 in honey bee RNA viruses has yet to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

The Dynamics of Deformed Wing Virus Concentration and Host Defensive Gene Expression after Varroa Mite Parasitism in Honey Bees, Apis mellifera

Zhao

Heerman

Peng

et al. 2019

Insects

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The synergistic interactions between the ectoparasitic mite Varroa destructor and Deformed wing virus (DWV) lead to the reduction in lifespan of the European honey bee Apis mellifera and often have been implicated in colony losses worldwide. However, to date, the underlying processes and mechanisms that form the multipartite interaction between the bee, mite, and virus have not been fully explained. To gain a better understanding of honey bees’ defense response to Varroa mite infestation and DWV infection, the DWV titers and transcription profiles of genes originating from RNAi, immunity, wound response, and homeostatic signaling pathways were monitored over a period of eight days. With respect to DWV, we observed low viral titers at early timepoints that coincided with high levels of Toll pathway transcription factor Dorsal, and its downstream immune effector molecules Hymenoptaecin, Apidaecin, Abaecin, and Defensin 1. However, we observed a striking increase in viral titers beginning after two days that coincided with a decrease in Dorsal levels and its corresponding immune effector molecules, and the small ubiquitin-like modifier (SUMO) ligase repressor of Dorsal, PIAS3. We observed a similar expression pattern for genes expressing transcripts for the RNA interference (Dicer/Argonaute), wound/homeostatic (Janus Kinase), and tissue growth (Map kinase/Wnt) pathways. Our results demonstrate that on a whole, honey bees are able to mount an immediate, albeit, temporally limited, immune and homeostatic response to Varroa and DWV infections, after which downregulation of these pathways leaves the bee vulnerable to expansive viral replication. The critical insights into the defense response upon Varroa and DWV challenges generated in this study may serve as a solid base for future research on the development of effective and efficient disease management strategies in honey bees.

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Transcriptomic response of the insect vector, Peregrinus maidis, to Maize mosaic rhabdovirus and identification of conserved responses to propagative viruses in hopper vectors

Cited by 26 publications

References 55 publications

A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes

A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes

Deep RNA Sequencing Reveals a Repertoire of Human Fibroblast Circular RNAs Associated with Cellular Responses to Herpes Simplex Virus 1 Infection

The Dynamics of Deformed Wing Virus Concentration and Host Defensive Gene Expression after Varroa Mite Parasitism in Honey Bees, Apis mellifera

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