Transcriptomics and metabolomics revealed that phosphate improves the cold tolerance of alfalfa

Wang, Yuntao; Sun, Zhen; Wang, Qiqi; Xie, Jianfei; Yu, Linqing

doi:10.3389/fpls.2023.1100601

Cited by 4 publications

(1 citation statement)

References 90 publications

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“…To overcome this stress factors, plants produce secondary metabolites together with other bioactive moleculesv (Rafinska et al 2017). In recent years, plants have treated with nanoparticles, hormones and combination of them to increase quality of the secondary metabolites or stress resistance (Sajid et al 2021;Paramo et al 2023;Wang et al 2023;Zhang et al 2023).…”

Section: Introductionmentioning

confidence: 99%

Callus Induction and Bioactive Compounds Production from Various Cultivars of Medicago sativa L. (alfalfa)

ALBAYRAK TURGUT,

BEZİRGANOGLU

2023

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Alfalfa (Medicago sativa L.) belongs to fabacaea family widely grown in Turkey. It is rich in bioactive compounds such as phenolic compounds, flavonoid, essential amino acids (threonine, leucine, lysine, and valine) and tannins, vitamins (A, B1, B2, B6, B12, C and E) or β-carotene. In this study, it was aimed to investigate the impact of secondary metabolite content of explants on callus biomass. For this purpose, cotyledon explants were obtained under sterile conditions, and transferred to standard MS medium containing 1 mg/L 2,4-D (Dichlorophenoxy Acetic Acid) and 0.0125 mg/L kinetin to induce callus formation. The phenolic, flavonoid and tannin contents of the explants were also determined. Leaves and cotyledons explants of 74 M. sativa L. cultivars have been used for callus biomass. The 74 tested alfalfa cultivars varied in their callus growth and callus biomass formation. Van-22, Konya-Ereğli, Alsancak, Gözlü-1 and Iside cultivars were observed with higher callus biomass: Conversely, Van Gevaş, Bitlis Hizan and Van-Çaldıran responded with lower callus biomass in tissue culture. A high-callus biomass cultivar of alfalfa has been shown to have higher total phenolic, flavonoid and tannin content activity than the lower-callus biomass cultivar in terms of leaf explants under tissue culture conditions. Total phenolic content activity was significantly increased in cotyledon explants with higher callus biomass as compared to lower callus biomass. The accumulation of leaf tannin and flavonoid was strongly linked to callus biomass. Cotyledon phenolic and flavonoid content exhibited an increasing trend in response to the increasing biomass of callus.

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Section: Introductionmentioning

confidence: 99%

Callus Induction and Bioactive Compounds Production from Various Cultivars of Medicago sativa L. (alfalfa)

ALBAYRAK TURGUT,

BEZİRGANOGLU

2023

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Phosphorus fertilization enhanced overwintering, root system and forage yield of late-seeded alfalfa in sodic soils

Wang,

Xie,

Fan

et al. 2024

Sci Rep

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Sowing date and soil fertility are very important factors in the overwintering and production performance of alfalfa (Medicago sativa L.), yet there’s a knowledge gap in knowledge on how late-seeded alfalfa responds to phosphorus (P) fertilization. A field study was conducted in Inner Mongolia from 2020 to 2022 using a split-plot design. The main plots consisted of five sowing dates (31 July, 8, 16, and 24 August, and 1 September), while the subplots involved five P application rates (0, 40, 70, 100, and 130 kg P2O5 ha−1). Throughout the growing seasons, the overwintering rate, root traits, forage yield, and yield components were measured. The results revealed a consistent decrease in overwintering ability and productivity with the delayed sowing. This reduction in overwintering rate was mainly due to diminished root traits, while the decrease in forage yield was largely associated with a reduction in plants per square meter. However, P fertilizer application to late-seeded alfalfa demonstrated potential in enhancing the diameter of both the crown and taproot, thus strengthening the root system and improving the overwintering rate, the rate of increase ranges from 11.6 to 49%. This adjustment could also improve the shoots per square meter and mass per shoot, increasing by 9.4–31.3% and 15.0–27.1% respectively in 2 years, which can offset the decline in forage yield caused by late sowing and might even increase the forage yield. Regression and path analysis indicated that alfalfa forage yield is primarily affected by mass per shoot rather than shoots per square meter. This study recommended that the sowing of alfalfa in similar regions of Inner Mongolia should not be later than mid-August. Moreover, applying P fertilizer (P2O5) at 70.6–85.9 kg ha−1 can enhance the forage yield and persistence of late-seeded alfalfa. Therefore, appropriate late sowing combined with the application of P fertilizer can be used as an efficient cultivation strategy for alfalfa cultivation after a short-season crop harvest in arid and cold regions.

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Increasing Phosphorus Application Level Alleviated Adverse Effects of Low-Temperature Stress on Antioxidant Metabolism and Carbohydrate Metabolism in Tobacco Seedlings

Xu,

Liu,

Wang

et al. 2024

Agronomy

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Low temperature, as a major abiotic stress, impacts the formation of high-quality tobacco seedlings. It is urgent to take appropriate measures to improve the low-temperature tolerance of tobacco seedlings. A hydroponics experiment was conducted with a tobacco cv. Y2001 under 25 °C (control temperature) and 10 °C (low-temperature stress). Three phosphorus (P) levels including the traditional P concentration (2 mM PO43−) and higher P levels (3 mM PO43− and 4 mM PO43−) were applied to investigate their effects on antioxidant metabolism and carbohydrate metabolism in low-temperature-stressed tobacco seedlings. The results showed that the low temperature decreased plant height, stem diameter, and biomass of shoots and roots, while the higher P levels promoted plant height and shoot biomass of low-temperature-stressed tobacco seedlings compared to the traditional P level. The leaf net photosynthetic rate (AN) was decreased by the low temperature, while the AN of low-temperature-stressed tobacco leaves was increased by 38.6–61.3% for the higher P levels than the traditional P level. Higher O2− and H2O2 were observed in tobacco leaves exposed to low-temperature stress, damaging the AN, although the low temperature upregulated the expression of encoding superoxide dismutase (NtSOD), peroxidase (NtPOD), and catalase (NtCAT). However, compared with the traditional P level, the higher P levels further upregulated the expression of NtSOD and NtCAT in low-temperature-stressed tobacco leaves to accelerate O2− and H2O2 removal. Higher leaf sucrose content was detected since the low temperature significantly downregulated the expression of NtSuSy, NtCWINV, and NtNINV encoding sucrose synthase, the cell wall, and alkaline invertases, respectively, inhibiting sucrose hydrolysis. Compared with the traditional P level, higher P levels downregulated the expression of NtCWINV in low-temperature-stressed tobacco leaves, further promoting leaf sucrose content. The low temperature downregulated the expression of NtAGP encoding ADP-glucose pyrophosphorylase, NtSSS encoding soluble starch synthase, and NtGBSS encoding granule-bound starch synthase, thereby restricting starch biosynthesis. Additionally, the low temperature upregulated the expression of α-amylase and β-amylase, accelerating starch hydrolysis. These led to a lower starch content in low-temperature-stressed tobacco leaves. The higher P levels further upregulated the expression of α-amylase in low-temperature-stressed tobacco leaves than the traditional P level, further lowering the starch content. Moreover, the leaf soluble sugar content was higher under the low temperature than the control temperature, which helped the tobacco plants resist low-temperature stress. And higher P levels further promoted the soluble sugar content in low-temperature-stressed tobacco leaves compared with the traditional P level, further improving tobacco seedlings’ low-temperature tolerance. Therefore, these results indicated that increasing the P application level can alleviate the adverse impacts of cold stress on antioxidant metabolism and carbohydrate metabolism in tobacco seedlings.

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Transcriptomics and metabolomics revealed that phosphate improves the cold tolerance of alfalfa

Cited by 4 publications

References 90 publications

Callus Induction and Bioactive Compounds Production from Various Cultivars of Medicago sativa L. (alfalfa)

Callus Induction and Bioactive Compounds Production from Various Cultivars of Medicago sativa L. (alfalfa)

Phosphorus fertilization enhanced overwintering, root system and forage yield of late-seeded alfalfa in sodic soils

Increasing Phosphorus Application Level Alleviated Adverse Effects of Low-Temperature Stress on Antioxidant Metabolism and Carbohydrate Metabolism in Tobacco Seedlings

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