Transcutaneous immunization by a solid-in-oil nanodispersion

Abstract

We have successfully achieved transcutaneous immunization without the use of any skin pre-treatment or immune-stimulant adjuvant by applying a solid-in-oil (S/O) nanodispersion: an oil-based nanodispersion of antigens coated with hydrophobic surfactant molecules. This finding indicates that the S/O nanodispersion has great promise for effective transcutaneous vaccination.

Transcutaneous Peptide Immunotherapy of Japanese Cedar Pollinosis Using Solid-in-Oil Nanodispersion Technology

“…Consequently, 7crpR had a higher affinity for sucrose laurate which hydrophile-lipophile balance value is 1. Our previous investigations demonstrated a mechanism of protein permeation through the S/O nanodispersion system: surfactant molecules were removed in the outermost SC layer and hydrophilic molecules only permeate into the hydrophilic layers in skin (20). Thus, inefficient removal of surfactant depressed the permeation of 7crpR into epidermis.…”

“…A S/O nanodispersion was prepared as previously reported (20). A 2.0-mL 7crpR aqueous solution (0.5 mg/mL) and a 4.0-mL L-195 cyclohexane solution (12.5 mg/mL) were homogenized with a polytron homogenizer (Kinematica AG, Luzern, Switzerland) at 26,000 rpm for 2 min to obtain a water-in-oil (W/O) emulsion.…”

“…S1). The S/O nanodispersion containing 7crpR was prepared following to our standard protocol (20,22), and was subjected to DLS analysis. As a result, the nanoparticle had a mean diameter of 230 nm with a polydispersity index of 0.250-0.281 in IPM (Fig.…”

“…Previously, our group proposed an original transcutaneous vaccine delivery technique based on a solid-in-oil (S/O) nanodispersion system. Using an antigen model protein, ovalbumin (OVA), the S/O nanodispersion system efficiently induced antigen-specific antibodies in murine models using a simple patch (20,21). The S/O nanodispersion is an oil-based dispersion of nano-sized particles containing protein/peptide molecules coated with surfactant molecules, which is formed by lyophilization of a water-in-oil emulsion and re-dispersion in an oil-vehicle.…”

Solid-in-oil dispersion: A novel core technology for drug delivery systems

“…The complex was easily dispersed into the oil to obtain a homogeneous and transparent oil-based protein dispersion, without loss of yield. According to previous studies, proteins cannot be dispersed as physical mixtures in oil, even in the presence of surfactant, and generally proteins precipitate in oil (Tahara et al, 2010, described this as a physical mixture). This indicated that the W/O emulsionmediated process played an important role in coating proteins with surfactants so that proteins would not precipitate.…”