Transduced Tat-Annexin protein suppresses inflammation-associated gene expression in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells

Abstract

Annexin-1 (ANX1) is an anti-inflammatory protein as well as

Suppression of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation in mice by transduced Tat-Annexin protein

“…Construction, purification and transduction of the Tat-ANX1 protein was previously described (20). In this study, we investigated how transduced Tat-ANX1 protein inhibited TPA-induced skin inflammation.…”

“…Expression and purification of the ANX1 fusion proteins were carried out as described previously (20). To produce the Tat-ANX1 proteins, the plasmid was transformed into E. coli BL21 cells (Novagen, Sandiego, CA, USA).…”

“…Among the cell-permeable peptides, Tat peptide is well known for its ability to deliver exogenous proteins http://bmbreports.org BMB Reports into cells (15). In previous studies, we have shown that a number of transduced fusion proteins including ANX1 efficiently protected against cell death in vitro and in vivo (16)(17)(18)(19)(20).…”

PEP-1-p18 prevents neuronal cell death by inhibiting oxidative stress and Bax expression

“…Although the transduction mechanism remains unclear, PTD fusion proteins have been used to deliver therapeutic proteins in vitro and in vivo. We have shown previously that various PTD fusion proteins transduced into neuronal cells cross the blood-brain barrier and efficiently protect against cell death (14)(15)(16)(17)(18)(19)(20). In this study, we investigated the protective effects of PEP-1-p18 against MPP + -induced dopaminergic neuronal cell death and MPTP-induced PD mouse models.…”

Mycobacterium tuberculosis 19-kDa lipoprotein induces Toll-like receptor 2-dependent peroxisome proliferator-activated receptor γ expression and promotes inflammatory responses in human macrophages

“…IL-6-deficient (IL-6 -/-) mice are resistant to the induction of various experimental inflammatory diseases (14). The mitogen-activated protein kinase (MAPK) pathway is important for immune response and mycobacterial pathogenesis (15)(16)(17), and MAPK family members include extracellular signal-regulated kinase, p38 MAPK (p38), and stress-activated protein kinase/c-Jun N-terminal kinase (18). It has been reported that p38 was activated in monocytes following M.tb infection (19).…”