Transepithelial<scp>d</scp>-glucose and<scp>d</scp>-fructose transport across the American lobster,<i>Homarus americanus</i>, intestine

Ijeoma, Eboagwu; Sterling, Kenneth; Ahearn, Gregory A.

doi:10.1242/jeb.055095

Cited by 24 publications

(20 citation statements)

References 37 publications

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“…Although the lobster diet is mainly protein, carbohydrates are encountered through marine plants (Conklin, 1995) and complex carbohydrate storage molecules such as glycogen in prey orangisms. Obi et al, (2011) reported transport of glucose and fructose across lobster intestine is qualitatively similar to sugar uptake in mammalian intestine, suggesting evolutionarily conserved absorption processes.…”

Section: Figurementioning

confidence: 76%

“…Also, the presence of the GLUT5-like protein from previous research indicated the brush border localization of this fructose transport protein to provide support for evolutionary transporter conservation of epithelial D-glucose and D-fructose transport by digestive organ systems (Obi et al, 2011). Additional experiments are needed to clarify this situation.…”

Section: Mucosal Serosalmentioning

confidence: 87%

“…The intestine is comprised of a single epithelial cell type with various transport proteins in the membranes of these cells and is considered to be a scavenger organ since it is secondary in nutrient absorption to the hepatopancreas (Wright and Ahearn, 1997). The intestine plays a significant role in the absorption of D-glucose and D-fructose (Verri et al, 2001 andObi et al, 2011). Carbohydrate digestion for these animals begins in the gastric chamber by secretion of a digestive enzyme mixture including α-amylase, glycosidases, maltase, and α-glucosidase (Johnson, 2003).…”

Section: Figurementioning

confidence: 99%

“…The apparent binding affinities for sugars in lobster and prawn intestines are reported to be in the low M range (Ahearn and Maginniss, 1977;Obi et al, 2011). It was noted that the crustacean intestine (posterior) is considered a scavenger organ that is resonsible for absorbing excess dietary sugars that were not otherwise absorbed by the hepatopancreas (anterior).…”

Section: Comparative Sugar Transportmentioning

confidence: 99%

“…includes SCRT-like, SGLT1-like, and GLUT5-like carrier systems on the brush border membrane and a proposed SCRT-like transporter, Na/K ATPase, and GLUT2-like carrier complex on the basolateral membrane. A GLUT-2 like transporter was found on the basolateral membrane to assist glucose and fructose transport from inside the epithelial cell to the blood (Obi et al, 2011). Although TLC did not indicate transport of 14 C-glucose and 14 C-fructose originating from 14 C-sucrose, the monomers could be formed at a concentration needed to fuel the cells themselves, producing the 15% volatile fraction of activity initially associated with the disaccharide (Figure 15).…”

Section: D-glucose Phloridzinmentioning

confidence: 99%

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Functional characterization of a putative disaccharide membrane transporter in crustacean intestine

2014

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Mechanisms of transepithelial absorption of dietary sucrose in the American lobster, Homarus americanus, were investigated to determine if disaccharide sugars can be transported across an animal gut intact. Intestines were mounted in a perfusion chamber to measure mucosal to serosal (MS) 14C‐sucrose transport. MS sucrose transport was a hyperbolic function of luminal sugar concentration, suggesting the presence of carrier‐mediated transport. Sodium‐dependent glucose transport inhibitor, phloridzin, partially decreased MS 14C‐sucrose transport, suggesting hydrolysis of 14C‐sucrose to glucose during transport. Phloretin, an inhibitor of Na+‐independent basolateral glucose transport, partially decreased MS 14C‐sucrose transport, suggesting that some 14C‐sucrose radioactivity may be transported to the blood as 14C‐glucose. Decreased MS 14C‐sucrose transport also occurred in the presence of the disaccharide trehalose. Thin‐layer chromatography (TLC) was used to identify the chemical nature of radioactively‐labeled sugars in the bath following transport and revealed that 14C‐sucrose was transported across the intestine largely as an intact molecule. Results suggest that disaccharide sugars may be transported intact across crustacean intestine and provide support for the occurrence of a disaccharide membrane transporter that has not previously been functionally characterized. Grant Funding Source: This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2010‐65

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Section: Figurementioning

confidence: 76%

Section: Mucosal Serosalmentioning

confidence: 87%

Section: Figurementioning

confidence: 99%

Section: Comparative Sugar Transportmentioning

confidence: 99%

Section: D-glucose Phloridzinmentioning

confidence: 99%

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Functional characterization of a putative disaccharide membrane transporter in crustacean intestine

2014

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Carbohydrate Transport—‘Life’s Useful Luxury Distributed’

Steinberg

2022

Aquatic Animal Nutrition

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Phenotypic flexibility in response to environmental salinity in the euryhaline crab Neohelice granulata from the mudflat and the saltmarsh of a SW coastal lagoon

2013

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This study constitutes a first attempt to investigate intraspecific differences in osmoregulatory capacity and digestive and metabolic responses at the biochemical level in relation to hyper-and hypo-regulation in a single species of estuarine crab inhabiting contrasting habitats within a same intertidal area. We compared hemolymph osmolality, key digestive enzymes, glycemia and energy reserves in Neohelice granulata (Dana in Proc Acad Nat Sci Philadelphia 5:247-254, 1851) from the mudflat and saltmarsh of Mar Chiquita coastal lagoon (37°32 0 /37°45 0 S-57°19 0 /57°26 0 W) under a wide range of salinities (6-60 psu). Individuals from both sites exhibited high and similar osmoregulatory capacity, but while in individuals from mudflat low and high salinities affected lipase activity in hepatopancreas and triglycerides in muscle, in crabs from saltmarsh, high salinities affected glycogen in anterior gills. Low salinity differentially affected free glucose in anterior gills. The results suggest the occurrence of intraspecific distinct digestive and metabolic adjustments in relation to osmoregulatory responses and habitat.

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Transepitheliald-glucose andd-fructose transport across the American lobster,Homarus americanus, intestine

Abstract: SUMMARY

Cited by 24 publications

References 37 publications

Functional characterization of a putative disaccharide membrane transporter in crustacean intestine

Functional characterization of a putative disaccharide membrane transporter in crustacean intestine

Carbohydrate Transport—‘Life’s Useful Luxury Distributed’

Phenotypic flexibility in response to environmental salinity in the euryhaline crab Neohelice granulata from the mudflat and the saltmarsh of a SW coastal lagoon

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