Transfection of cells attached to selected cell based biosensor surfaces

O’Connell, Daniel J.; Molinar, Arlette J.; Tavares, André Luiz Pasqua; Mathine, D.; Runyan, Raymond B.; Bahl, Joseph J.

doi:10.1016/j.lfs.2006.12.020

Cited by 2 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As one of the main cellular components for human tissues, fibroblasts are widely used to evaluate the cellular response of artificial biomaterials. , Here, typical fibroblast cell line COS-7 cells (derived from kidney cells of the African green monkey) were employed first to investigate the cell behaviors on all the chiral polymer brush films, considering that COS-7 has a symbolic branched cytoplasm and individual COS-7 cells can also migrate slowly over substrate, which would provide plentiful knowledge about the cell/substrate interactions. As we pay more attention to studying the cell/surface interactions, the fluorescent microscopy is preferred because of its intuitional manner, which focuses on the surface for studying cell behaviors in situ .…”

Section: Resultsmentioning

confidence: 99%

Modulating Cell Behaviors on Chiral Polymer Brush Films with Different Hydrophobic Side Groups

et al. 2012

View full text Add to dashboard Cite

Chirality is one of the significant biochemical signatures of life. Nearly all biological polymers are homochiral as they usually show high preference toward one specific enantiomer. This phenomenon inspires us to design biomaterials with chiral units and study their interactions with cells and other biological entities. In this article, through adopting three pairs of aliphatic amino acids with different hydrophobic side groups as chiral species, and using two adhesive cell lines as examples, we show that the chirality of polymer brushes can trigger differential cell behaviors on the enantiomorphous surfaces, and more interestingly, such chiral effect on cellular behaviors can be modulated in a certain extent by varying the hydrophobic side groups of the chiral moieties composing the polymers. This work not only proves the versatility of the chiral effect at the cell level but also demonstrates a method to bridge the gap between organic signal molecules and biomaterials. It thus points out a promising approach for designing novel biomaterials based on the chiral effect, which will be an important complement for conventional strategies in the study of biomaterials.

show abstract

Section: Resultsmentioning

confidence: 99%

Modulating Cell Behaviors on Chiral Polymer Brush Films with Different Hydrophobic Side Groups

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Due to the large dimension of cells compared to the short penetration depth of a biosensor, only the bottom portion of cells will be sensed. Mammalian cell attachment studies were conducted on a variety of common microchip surfaces for potential use in cell-based biosensors [17]. Data shows that there are differences in overall cell attachment between cell types but that most of the substrates will support attachment without the addition of any precoating protein and that transfection parameters are not significantly different between the surfaces that might be encountered in a biosensor.…”

Section: Cell-surface Interactionsmentioning

confidence: 99%

Optical biosensors for probing at the cellular level: A review of recent progress and future prospects

Velasco-Garcia

2009

Seminars in Cell & Developmental Biology

138

View full text Add to dashboard Cite

New approaches are required to understand the complex processes taking place in the smallest unit of life. Recent years have seen an increasing activity in the use of optical devices and techniques for the investigation of the properties of single cells and also populations of cells including cell to cell communication. This article reviews relevant optical technologies to date as well as new advances in biosensor development, and goes on to explore reported applications in detection of biotargets and cellular signalling pathways inside individual living cells.

show abstract

Transfection of cells attached to selected cell based biosensor surfaces

Cited by 2 publications

References 12 publications

Modulating Cell Behaviors on Chiral Polymer Brush Films with Different Hydrophobic Side Groups

Modulating Cell Behaviors on Chiral Polymer Brush Films with Different Hydrophobic Side Groups

Optical biosensors for probing at the cellular level: A review of recent progress and future prospects

Contact Info

Product

Resources

About