Transfection of
            <i>Staphylococcus aureus</i>
            with Bacteriophage Deoxyribonucleic Acid

Sjöström, Jan-Eric; Lindberg, Martin; Philipson, Lennart

doi:10.1128/jb.109.1.285-291.1972

Cited by 36 publications

(32 citation statements)

References 21 publications

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“…Strain 8325-4 can develop competence for transformation and transfection when harboring phage 441 either as a prophage or in a vegetative state (26). When bulk DNA from strain 8325(PI2) was used for transformation of strains 8325-4(414)thy and 8325-4(441) with erythromycin resistance as selective marker, both recipients were transformed, but the frequency of transformation was one order of magnitude higher with strain 8325-4(41l) as recipient.…”

Section: Resultsmentioning

confidence: 99%

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Thymineless bacteriophage induction in Staphylococcus aureus. I. High-frequency transduction with lysates containing a bacteriophage related to bacteriophage phi 11

Rudin¹,

Lindberg²

1975

J Virol

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A thymine-requiring mutant of Staphylococcus aureus, strain 8325(PIM)thy, undergoes prophage induction and lysis after thymine starvation. Four different phages were isolated from the lysate in low titers, among which was a phage designated 414, which differs from phage 441 in its immunity locus. The thymineless induced lysates of strain 8325(PIn8)thy transduce the penicillinase plasmid at high frequency (10-1), whereas transduction of chromosomal markers is inefficient. A plasmid-cured derivative of strain 8325(PI,.m)thy is also lysed by thymine starvation and can be used for high-frequency transduction of other plasmids. Reconstitution of a strain of S. aureus that responds to thymine starvation was only partially successful, but this system can effectively be used to transduce plasmids or plasmid derivatives. on July 7, 2020 by guest

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Section: Resultsmentioning

confidence: 99%

“…Transformation and transfection procedures. The methods for transformation (12) and transfection (26) have been described.…”

Section: Methodsmentioning

confidence: 99%

Thymineless bacteriophage induction in Staphylococcus aureus. I. High-frequency transduction with lysates containing a bacteriophage related to bacteriophage phi 11

Rudin¹,

Lindberg²

1975

J Virol

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“…Results on transfection of Staphylococcus aureus are quite different from those obtained with E. coli: there is no requirement for a 00C incubation step and the infective centers are sensitive to DNase for at least 20 min (363). However, competence for transfection (364) and genetic transformation (310) depend on one gene function in the chromosome of 411 prophage.…”

Section: Stepwise Analysis Of Transfectionmentioning

confidence: 94%

Transfection of Enterobacteriaceae and its applications.

Benzinger¹

1978

Microbiological Reviews

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“…Transformation in Staphylococcus has been described (7,8), and transfection has been used to determine the requirements for competence in this species (19,20). Competence is generally defined as the ability of a bacterial strain to take up biologically active deoxyribonucleic acid (DNA) and undergo transformation.…”

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confidence: 99%

Factors Affecting Competence for Transformation in Staphylococcus aureus

et al. 1974

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A chemically defined medium has been developed for isolation of amino acid-requiring mutants of Staphylococcus aureus strain 8325, and for use as a selective medium in transformation assays. Variables affecting transformation of both plasmid and chromosomal markers have been studied. The optimal pH and temperature for transformation are 6.75 to 7.0 and 30 C, respectively. Ca ions are required for transformation, and only cells lysogenic for the phage 011 can be transformed. Superinfection of competent cells with ,11 does not increase the transformation frequency. Maximal number of transformants is obtained after 20 min of contact between cells and deoxyribonucleic acid. The transformation frequencies for the plasmid marker erythromycin resistance (ero) and the chromosomal markers trp, thy, and cyt are of the same order of magnitude, whereas the frequency for the chromosomal marker tyr is approximately one order of magnitude lower. cedure for transformation, the recipient cells were grown on TSA plates and pyrimidine-requiring mutants were grown on TSA plates with added pyrimidine. Both cultures grew at 37 C overnight and then were suspended in TSB medium to an optical density at 524 nm (OD524) of 0.100, which equals 5 x 107 colony-forming units (CFU) per milliliter. The cell suspension was then diluted 10 times in TSB medium and incubated on a rotary shaker at 37 C. Maximal competence is reached after 1.5 to 2 h of growth (somewhat dependent on the strain used; see Fig. 1 and 2). The competent cells were washed once in 0.15 M NaCl and then suspended in 0.1 M tris(hydroxymethyl)aminomethane (Tris)-maleate buffer (pH 7.0) containing 0.1 M CaCl2 at a cell density of approximately 109 CFU/ml. In the transformation experiments, 0.9 ml of this cell suspension was mixed with 0.1 ml of DNA solution to give saturating concentration of DNA, i.e., 10 ug/ml (8). After incubation at 30 C for 20 min, the cells were centrifuged and suspended in 1 ml of TSB 155 on July 31, 2020 by guest

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Transfection of Staphylococcus aureus with Bacteriophage Deoxyribonucleic Acid

Cited by 36 publications

References 21 publications

Thymineless bacteriophage induction in Staphylococcus aureus. I. High-frequency transduction with lysates containing a bacteriophage related to bacteriophage phi 11

Thymineless bacteriophage induction in Staphylococcus aureus. I. High-frequency transduction with lysates containing a bacteriophage related to bacteriophage phi 11

Transfection of Enterobacteriaceae and its applications.

Factors Affecting Competence for Transformation in Staphylococcus aureus

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