Transfection of PNA–NLS Conjugates into Human Cells Using Partially Complementary Oligonucleotides

Aiba, Yuichiro; Ohyama, Junpei; Komiyama, Makoto

doi:10.1246/cl.150733

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…Upon the treatment with Ce(IV)/EDTA, the target site is selectively hydrolyzed under the conditions where unmodified pcPNAs hardly work. pcPNA-NLS conjugates can be spontaneously incorporated to various cells even without transfection agents, but the transfection is further promoted when they are combined with both a complementary oligonucleotide carrier and a lipofection agent [ 42 ]. The conjugates were efficiently localized in nuclei as expected.…”

Section: Second-generation Arcut For Still More Versatile Applicatmentioning

confidence: 99%

Applications of PNA-Based Artificial Restriction DNA Cutters

2017

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More than ten years ago, artificial restriction DNA cutters were developed by combining two pseudo-complementary peptide nucleic acid (pcPNA) strands with either Ce(IV)/EDTA or S1 nuclease. They have remarkably high site-specificity and can cut only one predetermined site in the human genome. In this article, recent progress of these man-made tools have been reviewed. By cutting the human genome site-selectively, desired fragments can be clipped from either the termini of chromosomes (telomeres) or from the middle of genome. These fragments should provide important information on the biological functions of complicated genome system. DNA/RNA hybrid duplexes, which are formed in living cells, are also site-selectively hydrolyzed by these cutters. In order to further facilitate the applications of the artificial DNA cutters, various chemical modifications have been attempted. One of the most important successes is preparation of PNA derivatives which can form double-duplex invasion complex even under high salt conditions. This is important for in vivo applications, since the inside of living cells is abundant of metal ions. Furthermore, site-selective DNA cutters which require only one PNA strand, in place of a pair of pcPNA strands, are developed. This progress has opened a way to new fields of PNA-based biochemistry and biotechnology.

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Section: Second-generation Arcut For Still More Versatile Applicatmentioning

confidence: 99%

Applications of PNA-Based Artificial Restriction DNA Cutters

2017

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“…High salt concentrations increase the stability of the DNA/DNA duplexes whilst slightly destabilising the PNA/DNA duplexes [36], which results in an overall decrease in invasion efficiency [37]. Several approaches have been attempted to enhance invasion efficiency in physiological salt concentrations, such as chemical modification of PNA and conjugation with functional molecules [37][38][39][40][41]. Our group developed conjugates of PNA with Ru-complexes and PNAs modified with a nuclear localisation signal (NLS) peptide to increase their applicability for DNA recognition at high salt concentrations (Figure 1b).…”

Section: Methodsmentioning

confidence: 99%

Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency

et al. 2020

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Peptide nucleic acid can recognise sequences in double-stranded DNA (dsDNA) through the formation of a double-duplex invasion complex. This double-duplex invasion is a promising method for the recognition of dsDNA in cellula because peptide nucleic acid (PNA) invasion does not require the prior denaturation of dsDNA. To increase its applicability, we developed PNAs modified with a nuclear localisation signal (NLS) peptide. In this study, the characteristics of NLS-modified PNAs were investigated for the future design of novel peptide-modified PNAs.

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Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics

Komiyama

Yoshimoto

Sisido

et al. 2017

Bulletin of the Chemical Society of Japan

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281

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In this review, we introduce two kinds of bio-related nanoarchitectonics, DNA nanoarchitectonics and cellmacromolecular nanoarchitectonics, both of which are basically controlled by chemical strategies. The former DNA-based approach would represent the precise nature of the nanoarchitectonics based on the strict or "digital" molecular recognition between nucleic bases. This part includes functionalization of single DNAs by chemical means, modification of the main-chain or side-chain bases to achieve stronger DNA binding, DNA aptamers and DNAzymes. It also includes programmable assemblies of DNAs (DNA Origami) and their applications for delivery of drugs to target sites in vivo, sensing in vivo, and selective labeling of biomaterials in cells and in animals. In contrast to the digital molecular recognition between nucleic bases, cell membrane assemblies and their interaction with macromolecules are achieved through rather generic and "analog" interactions such as hydrophobic effects and electrostatic forces. This cell-macromolecular nanoarchitectonics is discussed in the latter part of this review. This part includes bottom-up and top-down approaches for constructing highly organized cell-architectures with macromolecules, for regulating cell adhesion pattern and their functions in twodimension, for generating three-dimensional cell architectures on micro-patterned surfaces, and for building synthetic/natural macromolecular modified hybrid biointerfaces.

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Transfection of PNA–NLS Conjugates into Human Cells Using Partially Complementary Oligonucleotides

Cited by 5 publications

References 19 publications

Applications of PNA-Based Artificial Restriction DNA Cutters

Applications of PNA-Based Artificial Restriction DNA Cutters

Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency

Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics

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