Transfer across the perfused human placenta of antipyrine, sodium, and leucine

Schneider, H.; Panigel, M; Dancis, Joseph

doi:10.1016/0002-9378(72)90909-x

Cited by 459 publications

(221 citation statements)

References 7 publications

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“…Above R : 1.8, the increase in flow ratio produced little or no change in transfer in either foetal or maternal circulation. (Schneider et al, 1972). Further, it shows flow-dependent transfer of antipyrine from foetus to mother which was of smaller magnitude than that seen from mother to foetus.…”

mentioning

confidence: 85%

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Flow-dependent transfer of antipyrine in the human placenta in vitro

Challier¹,

d'Athis²,

Guerre-Millo³

et al. 1983

Reprod. Nutr. Dévelop.

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mentioning

confidence: 85%

“…This observation however does not implicate a concurrent arrangement in vivo where hemodynamic studies (Ramsey, 1968) (Faber, 1977). Schneider et al (1972) (Faber, 1969 (Stembera et al, 1965) for a foetus of 3.3 kg. The foeto-maternal flow ratio expected from these values is 0.50.…”

mentioning

confidence: 99%

Flow-dependent transfer of antipyrine in the human placenta in vitro

Challier¹,

d'Athis²,

Guerre-Millo³

et al. 1983

Reprod. Nutr. Dévelop.

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“…Consequently, various alternative models have been developed 6,7 . The most promising and probably most clinical relevant model is the ex vivo human placental perfusion model developed by Panigel and coworkers 2,3 .…”

Section: Introductionmentioning

confidence: 99%

“…The ex vivo dual recirculating human placental perfusion model provides a controlled and reliable system for studying the placental transport of various endogenous and exogenous compounds 3,11,12,18,19 and a wide range of other functions of the placenta like mechanisms responsible for the development of pathological states like preeclampsia [20][21][22] . In this protocol we focus mainly on the set up, handling and method that allow the study of accumulation, effects and translocation rates of a broad set of xenobiotics or nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Determination of the Transport Rate of Xenobiotics and Nanomaterials Across the Placenta using the <em>ex vivo</em> Human Placental Perfusion Model

Grafmüller

Manser

Krug

et al. 2013

JoVE

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Decades ago the human placenta was thought to be an impenetrable barrier between mother and unborn child. However, the discovery of thalidomide-induced birth defects and many later studies afterwards proved the opposite. Today several harmful xenobiotics like nicotine, heroin, methadone or drugs as well as environmental pollutants were described to overcome this barrier. With the growing use of nanotechnology, the placenta is likely to come into contact with novel nanoparticles either accidentally through exposure or intentionally in the case of potential nanomedical applications. Data from animal experiments cannot be extrapolated to humans because the placenta is the most species-specific mammalian organ 1 . Therefore, the ex vivo dual recirculating human placental perfusion, developed by Panigel et al. in 1967 2 and continuously modified by Schneider et al. in 1972 3 , can serve as an excellent model to study the transfer of xenobiotics or particles.Here, we focus on the ex vivo dual recirculating human placental perfusion protocol and its further development to acquire reproducible results.The placentae were obtained after informed consent of the mothers from uncomplicated term pregnancies undergoing caesarean delivery. The fetal and maternal vessels of an intact cotyledon were cannulated and perfused at least for five hours. As a model particle fluorescently labelled polystyrene particles with sizes of 80 and 500 nm in diameter were added to the maternal circuit. The 80 nm particles were able to cross the placental barrier and provide a perfect example for a substance which is transferred across the placenta to the fetus while the 500 nm particles were retained in the placental tissue or maternal circuit. The ex vivo human placental perfusion model is one of few models providing reliable information about the transport behavior of xenobiotics at an important tissue barrier which delivers predictive and clinical relevant data. Video LinkThe video component of this article can be found at

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“…En utilisant cette technique, Schneider, Panigel et Dancis (1972) ont établi que chez l'homme, le transfert placentaire de l'antipyrine était limité par les débits de perfusion foetal et maternel. Par ailleurs, il a été démontré chez l'animal, que l'antipyrine traverse facilement la membrane placentaire.…”

Section: Introductionunclassified