Transfer of Antibiotic Resistance Plasmid RP1 into Pseudomonas glycinea and Pseudomonas phaseolicola in vitro and in planta

Lacy, George H.; Leary, J. V.

doi:10.1099/00221287-88-1-49

Cited by 36 publications

(17 citation statements)

References 18 publications

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“…gbcinea to P. yringae pv. pbaseolicola in the phylloplane of bean was reported during the first 4 h after inoculation with an excess of donors (Lacy & Leary, 1975). Likewise, during the first hours after inoculation, P. syringae pv.…”

Section: Conjugal Transfer To Indigenous Bacteria In Plantamentioning

confidence: 90%

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High frequency of conjugation versus plasmid segregation of RP1 in epiphytic Pseudomonas syringae populations

et al. 1995

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The maintenance and transfer of the broad host-range plasmid RP1 in epiphytically growing populations of Pseudomnas syringae was monitored in the phyllosphere of bush bean (Phaseolus vulgaris). When foliage was inoculated with plasmid-containing bacteria, the plasmid was lost from the majority of the cells within 2 d but was stably maintained in 0 8 % of the population. A high frequency of conjugation between added donors and recipients was observed under high humidity conditions. In 1 d, the number of transconjugants rose to 10-1 of the donors and the proportional level of transconjugants continued to increase until 3 d after inoculation. Under these conditions the proportion of plasmid-containing bacteria stabilized at about 0 8 % of the total population. The conjugation rate appeared to be in equilibrium with plasmid loss and the slower growth of the plasmid-carrying cells. A factor that influenced the high conjugation frequency observed was the available nutrients provided by the leaf and also, to a lesser extent, the leaf surface itself. Transfer of the plasmid from added donors to indigenous bacteria was also studied, using a donor-specific bacteriophage for counterselection of the donor. Transfer was observed to 10 different species of Gram-negative epiphytically growing bacteria. The bean leaf surface appears to function as a hotspot at least for intraspecific transfer of plasmids in high humidity. The frequency of transfer was higher than in soil or in rhizosphere habitats. This is likely to be the result of an environment that is nutritionally rich in combination with a limited colonizable surface area which permits close contact between the bacterial cells.

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Section: Conjugal Transfer To Indigenous Bacteria In Plantamentioning

confidence: 90%

“…tomato contain conjugative plasmids (Bender & Cooksey, 1986 ;Powell e t al., 1993). High frequencies of conjugation between added donors and recipients on the leaf surface and in the leaf tissue have been reported (Lacy & Leary, 1975;Lacy e t al., 1984;Manceau et al, 1986).…”

Section: Introductionmentioning

confidence: 97%

High frequency of conjugation versus plasmid segregation of RP1 in epiphytic Pseudomonas syringae populations

et al. 1995

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“…versity of Michigan, Ann Arbor, MI 48104), has Further, in in vitro experiments, plasmids carrybeen used in previous studies (12,14). ing genes mediating resistance to tetracyclines, as Spontaneous mutants to OTC resistance.…”

Section: High Levels Of Streptomycin Resistance In Ementioning

confidence: 99%

Erwinia amylovoramutants and inplanta-derived transconjugants resistant to oxytetracycline

Lacy

Stromberg

Cannon

1984

Canadian Journal of Plant Pathology

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“…The rapid increase of these drug-resistant bacteria in agricultural fields including orchards and nurseries could be caused by plasmid-mediated transfer of drug-resistance genes among bacterial strains because most of these genes are encoded by native plasmids of plant pathogenic bacteria (Burr et al 1988;Cazorla et al 2002;Chiou and Jones 1991;Cooksey et al 1990;Minsavage et al 1990;Nakajima et al 2002;Sundin and Bender 1993). However, there is no information about in planta transfer of these native plasmids between bacterial strains on the phylloplane, although that of the nonnative plasmid, including RP1 (Lacy and Leary 1975), RSF1010 (Sato et al 1982), and transposon-inserted plasmid (pBPW1::Tn7), (Sato 1988;Sato et al 1985) has been reported.…”

Section: Introductionmentioning

confidence: 97%

Multidrug-resistance plasmid of Enterobacter cloacae : transfer to Erwinia herbicola on the phylloplane of mulberry and weeds

Sato

Wei

Watanabe

2003

Journal of General Plant Pathology

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A mulberry epiphytic Enterobacter cloacae MUL1 harbors plasmid pMUL1 encoding five drugresistance genes. This plasmid was examined upon its conjugal transfer into epiphytic Erwinia herbicola on the phylloplane of mulberry and 12 species of weeds. The plasmid was transferred into Er. herbicola at a frequency of 10 Ϫ5 -10 Ϫ3 /recipient in mulberry and Lolium multiflorum LAM. 1-8 days after wound inoculation with 10 6 -10 8 /ml suspensions. In Chenopodium album L. and C. album L. var. centrorubrum, however, it was transferred only after wound inoculation with a 10 8 /ml suspension, but not with 10 7 /ml or 10 6 /ml suspensions, owing to the weak epiphytic fitness of Ent. cloacae on these weeds. Transconjugants were also obtained for seven other species of weeds in the case of inoculation with a 10 8 /ml suspension. In contrast, when bacterial suspensions were sprayed on mulberry leaves with or without fresh wounds, transconjugants were obtained only in wounded leaves, which were considered suitable for bacterial conjugation. These findings suggest that epiphytic bacteria, including Ent. cloacae and Er. herbicola, may be carriers of drug-resistance genes distributed among plant pathogenic bacteria in nature.

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Transfer of Antibiotic Resistance Plasmid RP1 into Pseudomonas glycinea and Pseudomonas phaseolicola in vitro and in planta

Cited by 36 publications

References 18 publications

High frequency of conjugation versus plasmid segregation of RP1 in epiphytic Pseudomonas syringae populations

High frequency of conjugation versus plasmid segregation of RP1 in epiphytic Pseudomonas syringae populations

Erwinia amylovoramutants and inplanta-derived transconjugants resistant to oxytetracycline

Multidrug-resistance plasmid of Enterobacter cloacae : transfer to Erwinia herbicola on the phylloplane of mulberry and weeds

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