Transfer of nodulation ability in Rhizobium using R68.45 derived plasmids

Kowalczuk, E.; Skorupska, Anna; Lorkiewicz, Z

doi:10.1007/bf00270645

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…Neither strain was able to self-replicate RP4, but the frequencies of transfer were 105 times higher in DZ1. Also, plasmid R68.45 bearing two IS21 sequences, which is known to integrate in the chromosome of several gram-negative species at rather high frequencies (18,23,38), did not transfer to strain DZ1 more efficiently than did RP4, whereas R68.45 transferred 102 times more efficiently in strain DK101 than did RP4. These differences may come from physiological differences; strain DZ1 produces less slime, less lytic enzymes (that may kill the donor strain), and fewer or no restriction enzymes.…”

Section: Discussionmentioning

confidence: 94%

“…Although numerous phages of M. xanthus are known (21), no endogenous plasmid has been found, and few attempts to transfer exogenous plasmids have been reported (24,30).The plasmids of the P1-Inc group have a broad host range (7), being transferable to a wide variety of gram-negative bacteria. Some of them have been reported to mediate chromosome transfer (18, 37) or R-prime plasmid formation (17,19,23,26). The most frequently used plasmid RP4 (32) promotes chromosomal transfer in a limited number of cases (2, 36).…”

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confidence: 99%

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Transfer of plasmid RP4 to Myxococcus xanthus and evidence for its integration into the chromosome

Breton¹,

Jaoua²,

Guespin‐Michel³

1985

J Bacteriol

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The broad-host-range plasmid RP4 and its derivative R68.45 were transferred to Myxococcus xanthus DK101 and DZ1; RP4 was maintained integrated in the chromosome. Loss of plasmid markers occurred during the growth of the transconjugants, which could be prevented by selective pressure with oxytetracycline. The integrated plasmid was transferred back to Escherichia coli often as RP4-prime plasmids carrying various segments of the M. xanthus chromosome. It also mediated chromosomal transfer between M. xanthus strains.The bacterium Myxococcus xanthus has several remarkable traits. It is able to aggregate and differentiate into multicellular fruiting bodies (21) and produces antibiotics (29, 39) and proteins (26a) which are excreted into the surrounding medium. Currently, genetic manipulations use coliphage P1 to transfer Myxococcus xanthus genes previously cloned via pBR322 in Escherichia coli back to M. xanthus (28,34). Although numerous phages of M. xanthus are known (21), no endogenous plasmid has been found, and few attempts to transfer exogenous plasmids have been reported (24,30).The plasmids of the P1-Inc group have a broad host range (7), being transferable to a wide variety of gram-negative bacteria. Some of them have been reported to mediate chromosome transfer (18, 37) or R-prime plasmid formation (17,19,23,26). The most frequently used plasmid RP4 (32) promotes chromosomal transfer in a limited number of cases (2, 36). Therefore, different strategies have been designed to enhance chromosomal integration of RP4: (i) in vitro construction of an RP4-prime plasmid to allow integration by genetic recombination (3, 20); (ii) selection of a temperaturesensitive RP4 for maintenance (14), and (iii) use of transposable elements that mediate RP4 integration in the chromosome by cointegrate formation (19,38). R68.45, which is an RP4 plasmid exhibiting a duplicated IS21 (12,13), originally unique in RP4, falls into this class.In this paper, we show that RP4 can be transferred into M. xanthus where it is maintained integrated into the chromosome. It promoted the transfer of chromosomal markers between M. xanthus strains and R-prime plasmids when transferred back to E. coli. MATERIALS AND METHODSBacterial strains and culture conditions. The bacterial strains and plasmids used are listed in Table 1. M. xanthus was grown in CYE medium (Casitone [Difco Laboratories], 1%; yeast extract, [Biomerieux], 0.5%; MgSO4, 0.1%; pH 7.6) at 30°C. E. coli was grown in L broth (25) at 37°C. These media were solidified with 1.2% agar (Biomerieux).For M. xanthus, the antibiotics used and final concentrations (in milligrams per milliliter) were: kanamycin sulfate, 75 (Bristol Laboratories); oxytetracycline chloride, 12.5 (Roussel-Uclaf); carbenicillin, 500 (Beecham-Sevigne); nal-* Corresponding author. idixic acid, 100 (Sigma Chemical Co.), rifampin, 25 (Lepetit); streptomycin sulfate concentration as given below (Diamant). For E. coli, the antibiotics and their concentrations (in milligrams per milliliter) were: kanamycin sulfate, 25; tetr...

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Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

Transfer of plasmid RP4 to Myxococcus xanthus and evidence for its integration into the chromosome

Breton¹,

Jaoua²,

Guespin‐Michel³

1985

J Bacteriol

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“…lipoferum (12), Escherichia coli (45), Rhizobium leguminosarum (4), Rhizobium meliloti (30,34), Rhizobium trifolii (36), Zymomonas mobilis (22), and Methylophilus methylotrophus (22). R68.45 also promotes the formation of R-prime plasmids in Pseudomonas aeruginosa (20,42), Esch-erichia coli (23), R. leguminosarum (26), R. meliloti (29), R. trifolii (31), M. methylotrophus (B. W. Holloway, personal communication), Klebsiella pneumoniae (13), and Acinetobacter calcoaceticus (41).…”

mentioning

confidence: 99%

Chromosome transfer and R-prime plasmid formation mediated by plasmid pULB113 (RP4::mini-Mu) in Alcaligenes eutrophus CH34 and Pseudomonas fluorescens 6.2

Lejeune¹,

Mergeay²,

Gijsegem³

et al. 1983

J Bacteriol

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Plasmid pULB113 (RP4::mini-Mu), which contains the mini-Mu transposon, promoted both homologous and heterologous gene transfer from Pseudomonas fluorescens 6.2 and Alcaligenes eutrophus CH34. Homologous gene transfer in P. fluorescens 6.2 and A. eutrophus CH34 occurred at a frequency of 10-4 to 10-5, and recombinants inherited unselected recessive markers, suggesting a process of chromosome mobilization. Loci involved in autotrophic growth were among those transferred in A. eutrophus. In heterospecific matings, markers were transferred from P. fluorescens to A. eutrophus, Salmonella typhimurium LT2, and Escherichia coli, from A. eutrophus to P. fluorescens, and from Erwinia carotovora subsp. chrysanthemi to A. eutrophus. Heterospecific matings resulted in the formation of R-prime plasmids at frequencies of 10-7 to 10-4 per transferred plasmid. When S. typhimurium was the recipient, we observed R-prime plasmids with both restriction-proficient and restriction-deficient strains, although restriction markedly affected the frequency of transfer of pULB113. R-prime plasmids were quite stable, but lost the transposed marker more easily in a rec' back

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Structural analyses of lipid A from lipopolysaccharides of nodulating and non-nodulating Rhizobium trifolii

Russa

Lüderitz

Rietschel³

1985

Arch. Microbiol.

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Transfer of nodulation ability in Rhizobium using R68.45 derived plasmids

Cited by 7 publications

References 10 publications

Transfer of plasmid RP4 to Myxococcus xanthus and evidence for its integration into the chromosome

Transfer of plasmid RP4 to Myxococcus xanthus and evidence for its integration into the chromosome

Chromosome transfer and R-prime plasmid formation mediated by plasmid pULB113 (RP4::mini-Mu) in Alcaligenes eutrophus CH34 and Pseudomonas fluorescens 6.2

Structural analyses of lipid A from lipopolysaccharides of nodulating and non-nodulating Rhizobium trifolii

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