Transfer of superovulated sheep embryos obtained with different FSH-P

Torrès, Suzanne; Cognie, Y.; Colas, G.

doi:10.1016/0093-691x(87)90229-9

Cited by 59 publications

(30 citation statements)

References 9 publications

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“…After washing in fresh flushing medium, all ova and embryos were examined under an inverted microscope at magnification of 20-40 × (Leitz Labovert, Wetzlar, Germany) and classified according to their stage of development and morphology [33].…”

Section: Introductionmentioning

confidence: 99%

Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment

et al. 2001

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-In the non breeding period, the effect of two superovulatory treatments (eCG/FSH in single dose or FSH alone in four decreasing doses) on the production of embryo quality following in vitro viability after vitrification procedures was investigated using forty-four adult Sarda breed ewes. In sheep treated with eCG/FSH, the mean number of corpora lutea was significantly (P < 0.05) higher (11.8 ± 4.0 vs. 8.05 ± 3.8), although the recovery rate was significantly (P < 0.01) lower (74.6 vs. 59.9) than with FSH alone. After vitrification (ethylene glycol and glycerol) was repeated three times, the rates of re-expansion at first and second warming were significantly (P < 0.01) higher in embryos derived from FSH alone than in those with both gonadotrophins (94.9 and 41.9 vs. 72.8 and 18.6) and after the last vitrification the hatched blastocyst rates were 22.5 and 7.6. After differential stain, blastocysts derived from FSH alone showed a mean number of cells significantly higher than blastocysts from eCG/FSH (184.2 vs. 157.7). It was concluded that superovulatory treatment with eCG/FSH may increase the ovarian responses compared with FSH alone, but these embryos showed a reduction in viability rates after repeated vitrification. superovulation / embryo quality / ovine / vitrification / viabilityRésumé -Les embryons ovins obtenus après un traitement de superovulation associant FSH et eCG ont une viabilité in vitro plus faible après vitrification que les embryons obtenus avec un traitement séquentiel de FSH. Pendant la saison non reproductive, on a évalué, chez 44 brebis adultes de race Sarde, l'effet de deux traitements de superovulation (le premier avec les eCG/FSH en une seule administration, le deuxième avec la FSH administrée en quatre doses décroissantes) sur la qualité des embryons produits et sur la viabilité in vitro après vitrification. Dans le groupe traité avec les eCG/FSH, le nombre moyen d'ovulations était plus élevé (11,8 ± 4,0 vs. 8,05 ± 3,8, P < 0,05) tandis que le taux de récupération était plus faible (74,6 vs. 59,9, P < 0,01) par rapport au groupe traité avec la FSH seule. Les embryons ont suivi 3 vitrifications successives (éthylène glycol et glycérol). Reprod. Nutr. Dev. 41 (2001) [239][240][241][242][243][244][245][246] 239

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Section: Introductionmentioning

confidence: 99%

Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment

et al. 2001

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“…Furthermore, a high dose of eCG induces many anovulatory follicles [7]. Multiple injections of a high dose of FSH combined with a low dose of eCG is now conventionally used in ewes to induce superovulation [2,4,8], as it can induce a higher ovulatory response than the multiple injections of FSH alone [9]. However, the use of multiple injections, such as 6 times during 2 or 3 days, increases the workload as well as causes stress in animals.…”

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“…Dev. 44: [169][170][171][172][173][174][175][176] 1998) is necessary to inject it repeatedly [1], and the administration of decreasing doses gave more successful results than that administrating of a constant dose [2]. Multiple injections of FSH in decreasing doses resulted in increasing the number of corpora lutea, the number of recovered SH or eCG are gonadotropins widely utilized for induction of superovulation in ewes.…”

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confidence: 99%

A Simple Superovulation Method of A Single Injection of Follicle-Stimulating Hormone Combined with Equine Chorionic Gonadotropin for Superovulation of Suffolk Ewes During the Breeding Season: I. Effects of Different Treatments on the Endocrine Profiles.

Watanabe¹,

Miyamoto²,

Okada³

et al. 1998

Journal of Reproduction and Development

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Abstract. Two trials were conducted to investigate a simple superovulation method for Suffolk ewes during the breeding season. The method involves a single injection of porcine follicle-stimulating hormone (pFSH) combined with equine chorionic gonadotropin (eCG) and was evaluated by measuring the concentrations of plasma hormones (estradiol-17β (E 2 ), progesterone (P 4 ), luteinizing hormone (LH) and follicle-stimulating hormone (FSH)). The ewes were treated with a vaginal sponge containing synthetic steroid with progestative action (P sponge) for 12 days in both trials. In Trial 1 (conducted in 1995), 18 ewes received a single injection (F1) or multiple injections (F6) of a total of 20 mg pFSH, and in combination with an injection of 500 IU eCG (F1+P, F6+P). The injections were conducted on Day 2 (Day 0 was the day of P sponge removal). In trial 2 (conducted in 1996), 24 ewes were given single injections of pFSH and eCG at different times; (i) both hormones on Day 2 (F-2/P-2), (ii) pFSH on Day -2 and eCG on Day 1 (F-2/P-1), and (iii) both hormones on Day 1 (F-1/P-1). One hundred micrograms of an analogue of gonadotropin-releasing hormone (GnRH) was administered on Day 1 in Trial 1 and at the detection of estrus in Trial 2. Frozen-thawed semen was inseminated in utero using a laparoscope at 30 h and 38 h after P sponge removal in Trial 1 and at 18 hours after the detection of estrus in Trial 2. Blood samples were collected from the jugular vein. E 2 , P 4 , LH and FSH were assayed by second-antibody enzyme immunoassays. F1+P showed similar endocrine profiles to those of F6 and F6+P with a preovulatory E 2 peak after P sponge removal, but F1 did not show a clear preovulatory E 2 peak. The E 2 concentration in F-2/P-1 rose biphasically; there was a rise just after injection of pFSH and then another rise after P sponge removal. Simultaneous injections of 20 mg pFSH and 500 IU eCG may reduce the E 2 elevation. We concluded that a good ovarian response comparable to the multiple injection method can be achieved by the present simple superovulation method. Key words: pFSH, eCG, Superovulation, Ewes, Endocrine profiles.(J. Reprod. Dev. 44: [169][170][171][172][173][174][175][176] 1998) is necessary to inject it repeatedly [1], and the administration of decreasing doses gave more successful results than that administrating of a constant dose [2]. Multiple injections of FSH in decreasing doses resulted in increasing the number of corpora lutea, the number of recovered SH or eCG are gonadotropins widely utilized for induction of superovulation in ewes. Be-

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“…In a previous study (Torres et al, 1987) (PMSG) show a substantial decline in ovarian response at a second treatment given 7 to 9 weeks after the first (Saumande and Chupin, 1977 …”

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Repeated superovulation and surgical recovery of embryos in the ewe

Torrès

Sevellec²

1987

Reprod. Nutr. Dévelop.

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Summary. Repeated superovulation using three treatments of pFSH at intervals of 45 to 55 days during the same breeding season was carried out in 18 Prdalpes ewes.The embryos were recovered by surgery after the first two treatments and by slaughter after the last one at Day 6. Three lots of control ewes In = 5 or 6) receiving the same superovulation treatment were slaughtered respectively at the same time.The first and third repeated superovulation treatments gave averages of 5.2 + 0.6 and 4.5 ± 0.6 corpora lutea. The second one gave 3.4 t 0.4 corpora lutea, which was significantly lower than with treatments 1 and 3, but adhesions did not permit a perfect view of the whole surface of the ovaries. Introduction.

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Transfer of superovulated sheep embryos obtained with different FSH-P

Cited by 59 publications

References 9 publications

Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment

Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment

A Simple Superovulation Method of A Single Injection of Follicle-Stimulating Hormone Combined with Equine Chorionic Gonadotropin for Superovulation of Suffolk Ewes During the Breeding Season: I. Effects of Different Treatments on the Endocrine Profiles.

Repeated superovulation and surgical recovery of embryos in the ewe

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