Transfer RNA genes ofZea mays chloroplast DNA

Selden, Richard F.; Steinmetz, André; McIntosh, Lee; Bogorad, Lawrence; Burkard, G.; Mubumbila, Mfika; Kuntz, Marcel; Crouse, Edwin J.; Weil, Jacques-Henry

doi:10.1007/bf01578514

Cited by 27 publications

(27 citation statements)

References 34 publications

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“…A total of 30 tRNA genes were identified using these individual recombinant clones of ctDNA. This number is very close to that reported for Pisum sativum ctDNA (14) and to those found in other organisms (5,9,19,20).…”

Section: N U M B E R O F T R N a Genes In Pisum Sativum C T D N Asupporting

confidence: 86%

“…tRNA genes also have been localized in the spacer region of Spinacea oleracea and Zea mays ctDNA (1,5). In addition, tRNAs have been found near the 5' end of the 16S rRNA gene and near the 3' end of the 5S rRNA gene in Zea rnays (20). In Spinacea oleracea, it has been possible to identify tRNA ue in the spacer region (5).…”

Section: Discussionmentioning

confidence: 96%

“…The tRNAs for aminoacids cysteine, methionine and glutamine were not detected. The inability to localize the genes for three distinct aminoacyl tRNAs in ctDNA of Zea mays and Pisum sativum probably result from inadequate charging of the tRNAs rather than the lack of their genes in ctDNA (14,20). It was shown that there are about 25 tRNA genes in Euglena gracilis (19).…”

Section: Introductionmentioning

confidence: 96%

“…Spinach tRNAs were fractionated into about 35 spots by two dimensional gel electrophoresis, and 21 tRNA genes were located (5). Recently, 37 tRNA genes have been located in Zea mays chloroplasts (20).…”

Section: Introductionmentioning

confidence: 99%

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Distribution of transfer RNA genes in thePisum sativum chloroplast DNA

et al. 1985

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Purified chloroplast tRNAs were isolated fromPisum sativum leaves and radioactively labeled at their 3' end using tRNA nucleotidyl transferase and α(32)P-labeled CTP. Pea ctDNA was fragmented using a number of restriction endonucleases and hybridized with thein vitro labeled chloroplast tRNAs by DNA transfer method. Genes for tRNAs have been found to be dispersed throughout the chloroplast genome. A closer analysis of the several hybrid regions using recombinant DNA plasmids have shown that tRNA genes are localized in the chloroplast genome in both single and multiple arrangements. Two dimensional gel electrophoresis of total ct tRNA have identified 36 spots. All of them have been found to hybridize withPisum sativum ctDNA. Using recombinant clones, 30 of the tRNA spots have been mapped inPisum sativum ctDNA.

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Section: N U M B E R O F T R N a Genes In Pisum Sativum C T D N Asupporting

confidence: 86%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Distribution of transfer RNA genes in thePisum sativum chloroplast DNA

et al. 1985

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“…Hybridisation of individually identified tRNA species to plastid DNA has been performed in a number of plants (43). For comparison with barley, the most relevant species is maize (37).…”

Section: Expression Of the Trna Genesmentioning

confidence: 99%

Structure of a heavily transcribed region of barley chloroplast DNA. Transfer RNA genes for serine (UGA), glycine (GCC, UCC), formyl-methionine and threonine (GGU)

Oliver

Poulsen

1984

Carlsberg Res. Commun.

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The barley chloroplast genome is transcribed into at least 80 RNA molecules (29). Some regions of the genome hybridise to more transcripts than others. One 4.3 kbp HindlII fragment was found to hybridise to a disproportionately high number of transcripts, some of which were light-induced. The sizes of the transcripts ranged from 0.3 to 5.5 kb. In order to discover from which genes these transcripts are derived, the DNA sequence of the fragment has been determined and the transcripts have been mapped. Five tRNA genes have been located including genes for formyl-methionine tRNA and a glycine tRNA with an intron in the D-stem. A scheme to account for the transcription and processing of a precursor-transcript for these tRNAs is proposed. A region containing two open-reading frames hybridises to 9 transcripts. The largest of these, which is 5.5 kb, may be intereistronically processed. A number of long repeated sequences which may be involved in recombinational activity were found.

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