Transfer to Processing Tomato and Characterization of Late Blight Resistance Derived from Solanum pimpinellifolium L. L3708

Kim, Min-Jea; Mutschler, Martha A.

doi:10.21273/jashs.130.6.877

Cited by 39 publications

(21 citation statements)

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“…Phytophthora infestans is a biotrophic pathogen that is the causal agent of late blight (LB) in tomato. LB occurs worldwide and has long been prevalent in some countries, causing serious economic loss for field-grown tomatoes (Kim and Mutschler, 2005;Miranda et al, 2010); it is therefore regarded as a major threat to tomato production (Park et al, 2013). During the growing season of tomato, LB can occur at any time, which leads to brown-black, pathogen sporulation and water-soaked lesions on the foliage, stems and fruits of tomato (Fry et al, 1992;Park et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis between resistant and susceptible tomato allows the identification of lncRNA16397 conferring resistance to Phytophthora infestans by co‐expressing glutaredoxin

et al. 2017

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The rapid development of omics sequencing technology has facilitated the identification of thousands of long non-coding (lnc)RNAs in plant species, but the role of lncRNAs in plant-pathogen interactions remains largely unexplored. We used comparative transcriptome analysis of Phytophthora infestans-resistant and -susceptible tomatoes to identify differentially expressed genes (DEGs) and lncRNAs (DELs), and examine lncRNA-mRNA networks. A total of 1037 DEGs and 688 DELs were identified between P. infestans-resistant and -susceptible tomatoes. The co-localization networks, including 128 DEGs and 127 DELs, were performed. We found that lncRNA16397 acted as an antisense transcript of SlGRX22 to regulate its expression, and also induced SlGRX21 expression when lncRNA16397 was overexpressed. In addition, disease symptoms and reactive oxygen species (ROS) accumulation in tomatoes overexpressing lncRNA16397 and SpGRX were fewer and lower than those in wild-type after P. infestans infection. This result suggests that tomato lncRNA16397 induces SlGRX expression to reduce ROS accumulation and alleviate cell membrane injury, resulting in enhanced resistance to P. infestans. Our results provide insight into lncRNAs involved in the response of tomato to P. infestans infection, demonstrate that the lncRNA16397-GRXs network is an important component of the P. infestans network in tomato, and provide candidates for breeding to enhance biotic stress-resistance in tomato.

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Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis between resistant and susceptible tomato allows the identification of lncRNA16397 conferring resistance to Phytophthora infestans by co‐expressing glutaredoxin

et al. 2017

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“…The resistance of L3708 was transferred into a series of processing tomato lines by the Cornell Univ. breeding program (Kim, 2003;Kim and Mutschler, 2005), using, as the initial starting material, two late blight-resistant BC 1 F 2 plants ('97A46' and '97A48', respectively) provided by R. Gardner. Lines '97A46' and '97A48' are BC 1 F 2 plants from the pedigree 'NC215E' x ('NC215E' x S. pimpinellifolium L 3708) selected as late blight resistant under natural infection (R. Gardner, personal communication).…”

Section: Tomato Lines Testedmentioning

confidence: 99%

“…of Agriculture (USDA) accessions NSL116890 and PI365957] as having strong resistance to late blight (AVRDC, 1994;Chunwongse et al, 2002). They generously provided L3708 to other breeding programs, which proceeded to transfer the resistance to tomato lines (AVRDC, 1999;Kim, 2003;Kim and Mutschler, 2005). When the resulting lines were grown together under natural infection, their degree of late blight resistance differed among the lines bred by different programs (R. Gardner, personal communication).…”

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confidence: 99%

“…When the resulting lines were grown together under natural infection, their degree of late blight resistance differed among the lines bred by different programs (R. Gardner, personal communication). Segregation data obtained while breeding with this source of late blight resistance also suggested the possibility of differential response of homozygous vs. heterozygous plant genotypes to several pathogen isolates (Kim, 2003;Kim and Mutschler, 2005). A more complete examination of these differences in disease response could help design optimal breeding strategies for transfer of the broadest resistance possible using this source of resistance, and determine the most reliable and stable means to deploy this resistance in commercial cultivars.…”

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confidence: 99%

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Characterization of Late Blight Resistance Derived from Solanum pimpinellifolium L3708 against Multiple Isolates of the Pathogen Phytophthora infestans

Kim¹,

Mutschler²

2006

jashs

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Sixteen tomato [Solanum lycopersicum L. (syn. Lycopersicon esculentum Mill.)] genotypes (inbred lines or hybrids) were tested against five Phytophthora infestans (Mont.) deBary isolates to characterize race specificity of late blight resistance transferred to tomato from Solanum pimpinellifolium L. [syn. Lycopersicon pimpinellifolium (L.) Mill.] accession L3708. The effects of plant genotype, isolate, genotype × isolate, and isolate × replication interactions were highly significant (P = 0.001). Set of four sister lines fixed for late blight resistance (CU-R lines) exhibited full and equal resistance to the five pathogen isolates tested. In contrast, the heterozygous F1 hybrids, created by crossing the resistant CU-R lines with a susceptible parent, were resistant to US-11; partially resistant to US-17, NC-1, and DR4B; and susceptible to US-7. Differential responses were also observed across pathogen isolates on a set of resistant sister lines (CLN-R lines), which also were bred from L3708. The CLN-R lines were resistant to the DR4B, NC-1, and US-11 isolates, but showed significant disease-affected areas and sporangium numbers following inoculation with either US-7 or US-17. Restriction fragment length polymorphism (RFLP) analysis confirms that both CU-R and CLN-R are homozygous for the Ph-3 gene derived from L3708. Since progeny tests also confirmed that the CLN-R lines are fixed for their level of resistance, these results suggest that late blight resistance in the CU-R lines is not controlled by Ph-3 alone, and that at least one additional gene conferring late blight resistance is missing from the CLN-R lines. Results of genetic tests of the (CU-R × CLN-R) F1 and a (CU-R × CLN-R) F2 population with the pathogen isolate US-17 strongly support a model in which resistance of the CU-R lines requires genes in addition to Ph-3. The implications of this information in breeding for late blight resistance and using of the resulting resistant lines or hybrids are discussed.

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“…The work of Chen et al (2008) indicated that Ph-2 and Ph-3 complement each other and together offer resistance to a broader range of pathogen isolates compared to either gene alone. Evidence from Cornell University supported the presence of a second late blight resistance gene in L3708 Mutschler 2005, 2006) which interacted epistatically with Ph-3 (Kim and Mutschler 2005). Recently, Chen et al (2014) mapped qPh2.1 on chromosome 2 of L3708, which also contributed to late blight resistance.…”

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confidence: 98%

Evaluation of the Ph‐3 gene‐specific marker developed for marker‐assisted selection of late blight‐resistant tomato

et al. 2016

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Late blight caused by Phytophthora infestans is one of the most destructive diseases of tomato (Solanum lycopersicum L.) that mainly occurs in cool and wet environments. With the spread of the A2 mating type and new clonal lineages, fewer fungicides provide effective control of the disease, which has increased its worldwide threat. Host resistance could contribute significantly to sustainable disease control. Ph-3 is a race-specific late blight resistance gene commonly used in commercial tomato breeding. Availability of precise and easy to use gene-based markers would facilitate selection. In this study, a Ph-3 on-gene cleaved amplified polymorphic sequence (CAPS) marker, Ph3.gsm/HincII, was developed based on the published gene sequence of Ph-3. The effectiveness of the marker was evaluated along with other published Ph-3 markers using an F 9 recombinant inbred line (RIL) population derived from NC 23E-2 (93) 9 L3708. Markers Ph3.gsm/HincII and TG328/BstNI accurately genotyped the RIL population for Ph-3. In addition, Ph3.gsm/HincII was able to differentiate variable susceptible alleles. This reliable codominant DNA marker would be very useful in marker-assisted selection, particularly for resistance gene pyramiding.

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Transfer to Processing Tomato and Characterization of Late Blight Resistance Derived from Solanum pimpinellifolium L. L3708

Cited by 39 publications

References 12 publications

Comparative transcriptome analysis between resistant and susceptible tomato allows the identification of lncRNA16397 conferring resistance to Phytophthora infestans by co‐expressing glutaredoxin

Comparative transcriptome analysis between resistant and susceptible tomato allows the identification of lncRNA16397 conferring resistance to Phytophthora infestans by co‐expressing glutaredoxin

Characterization of Late Blight Resistance Derived from Solanum pimpinellifolium L3708 against Multiple Isolates of the Pathogen Phytophthora infestans

Evaluation of the Ph‐3 gene‐specific marker developed for marker‐assisted selection of late blight‐resistant tomato

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